cDNA Clone NIK

• Plasmid: pCMV-HA-1 NIK D534A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA NIK D534A
• Genbank: Q99558
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGT
GGGGCAGCAGAAGGAACTCCCCAAGCCAAAGGAGAAGACGCCGCCACTGG
GGAAGAAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCT
GTGTTCTGCGGAAAGTGGGAGATCCTGAATGACGTGATTACCAAGGGCAC
AGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGCCATCTCTATCATCGCCC
AGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTCAGAACGC
ATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCA
GATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGT
GTTGGAAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAG
AGCTCAAAGTCCCTGGCTCATGCAGGAGTGGCCTTGGCCAAACCCCTCCC
CAGGACCCCTGAGCAGGAGAGCTGCACCATCCCAGTGCAGGAGGATGAGT
CTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCACCAAGCCT
CTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGG
CCTACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCT
TGCAATGTCTGAACCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGC
CCCCTGCCCCTGCCCACGCACCCCTTCCCCTATAGCAGACTGCCTCATCC
CTTCCCATTCCACCCTCTCCAGCCCTGGAAACCTCACCCTCTGGAGTCCT
TCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCCTGACCCA
CACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCC
ACACCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTG
TGGAGGAATACCTAGTGCATGCTCTGCAAGGCAGCGTGAGCTCAAGCCAG
GCCCACAGCCTGACCAGCCTGGCCAAGACCTGGGCAGCAAGGGGCTCCAG
ATCCCGGGAGCCCAGCCCCAAAACTGAGGACAACGAGGGTGTCCTGCTCA
CTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGTCCACTGG
GCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAG
GATGGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGC
TGGAAGTATTTCGGGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCA
CCCAGAATTGTCCCTTTGTATGGAGCTGTGAGAGAAGGGCCTTGGGTCAA
CATCTTCATGGAGCTGCTGGAAGGTGGCTCCCTGGGCCAGCTGGTCAAGG
AGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGGCCAGGCC
CTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGT
CAAAGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCT
GTGCCTTTGGCCATGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCC
TTGCTCACAGGGGACTACATCCCTGGCACAGAGACCCACATGGCTCCGGA
GGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGTGGATGTCTGGAGCAGCT
GCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGACTCAGTTC
TTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAG
GGAGATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGG
GGCTGAGGAAAGAGCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGG
AAGGTGAACCGGGCACTACAGCAAGTGGGAGGTCTGAAGAGCCCTTGGAG
GGGAGAATATAAAGAACCAAGACATCCACCGCCAAATCAAGCCAATTACC
ACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGCCCCAGGG
CCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCC
TCTCCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGA
GCAAGGAGGAGTCTGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAG
CCAGCCCCTGCCAGAAACCCCAGCTCACCAGAGCGGAAAGCAACCGTCCC
GGAGCAGGAACTGCAGCAGCTGGAAATAGAATTATTCCTCAACAGCCTGT
CCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTGCCTCAGC
ATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGC
CTCTCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCA
GCCAGGCCGAGGCTCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGG
CGGCCCACCGACACCCCAAGCTATTTCAATGGTGTGAAAGTCCAAATACA
GTCTCTTAATGGTGAACACCTGCACATCCGGGAGTTCCACCGGGTCAAAG
TGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGCAGCCTTC
AGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCC
AGACTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCG
CCTGGAGCTGGAGGGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAAGAA
TTC

Amino Acid Sequence

MYPYDVPDYAGSAVMEMACPGAPGSAVGQQKELPKPKEKTPPLGKKQSSV
YKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENS
QEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRR
SKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPY
VRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHV
WKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLAC
VDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVH
ALQGSVSSSQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPV
DYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAE
ELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPE
DRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCAFGHAV
CLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHM
LNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPI
HRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQ
PRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGM
WEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLE
EQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARS
SSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATG
ISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVK
HGQLENRP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15872
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; ; Estimated MW of expressed protein : 105277 Daltons