cDNA Clone GFAT1

• Plasmid: pFBDual GFAT 1-300 6His 301-681
• Parent Plasmid: pFastbacDual
• Expressed: GFAT 1-300 6His 301-681
• Genbank: BC045641
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGAAACCATGTGTGGTATATTTGCTTACTTAAACTACCATGTTCC
TCGAACGAGACGAGAAATCCTGGAGACCCTAATCAAAGGCCTTCAGAGAC
TGGAGTACAGAGGATATGATTCTGCTGGTGTGGGATTTGATGGAGGCAAT
GATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATTAAGAAGAA
AGGAAAAGTTAAGGCACTGGATGAAGAAGTTCACAAGCAACAAGATATGG
ATTTGGATATAGAATTTGATGTACACCTTGGAATAGCTCATACCCGTTGG
GCAACACATGGAGAACCCAGTCCTGTCAATAGCCACCCCCAGCGCTCTGA
TAAAAATAATGAATTTATCGTTATTCACAATGGAATCATCACCAACTACA
AAGACTTGAAAAAGTTTTTGGAAAGCAAAGGCTATGACTTCGAATCTGAA
ACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTATGACAATCG
GGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAAC
AATTGGAAGGTGCTTTTGCACTTGTGTTTAAAAGTGTTCATTTTCCCGGG
CAAGCAGTTGGCACAAGGCGAGGTAGCCCTCTGTTGATTGGTGTACGGAG
TGAACATAAACTTTCTACTGATCACATTCCTATACTCTACAGAACAGGCA
AAGACAAGAAAGGAAGCTGCAATCTCTCTCGTGTGGACAGCACAACCTGC
CTTTTCCCGGTGGAAGAAAAAGCAGTGGAGTATTACTTTGCTTCTGATGC
AAGTGCTGTCATAGAACACACCAATCGCGTCATCTTTCTGGAAGATGATG
ATGTTGCAGCAGTAGTGGATGGACGTCTTTCTATCCATCGAATTAAACGA
ACTGCAGGAGATCATCATCACCATCACCATCACCCCGGACGAGCTGTGCA
AACACTCCAGATGGAACTCCAGCAGATCATGAAGGGCAACTTCAGTTCAT
TTATGCAGAAGGAAATATTTGAGCAGCCAGAGTCTGTCGTGAACACAATG
AGAGGAAGAGTCAACTTTGATGACTATACTGTGAATTTGGGTGGTTTGAA
GGATCACATAAAGGAGATCCAGAGATGCCGGCGTTTGATTCTTATTGCTT
GTGGAACAAGTTACCATGCTGGTGTAGCAACACGTCAAGTTCTTGAGGAG
CTGACTGAGTTGCCTGTGATGGTGGAACTAGCAAGTGACTTCCTGGACAG
AAACACACCAGTCTTTCGAGATGATGTTTGCTTTTTCCTTAGTCAATCAG
GTGAGACAGCAGATACTTTGATGGGTCTTCGTTACTGTAAGGAGAGAGGA
GCTTTAACTGTGGGGATCACAAACACAGTTGGCAGTTCCATATCACGGGA
GACAGATTGTGGAGTTCATATTAATGCTGGTCCTGAGATTGGTGTGGCCA
GTACAAAGGCTTATACCAGCCAGTTTGTATCCCTTGTGATGTTTGCCCTT
ATGATGTGTGATGATCGGATCTCCATGCAAGAAAGACGCAAAGAGATCAT
GCTTGGATTGAAACGGCTGCCTGATTTGATTAAGGAAGTACTGAGCATGG
ATGACGAAATTCAGAAACTAGCAACAGAACTTTATCATCAGAAGTCAGTT
CTGATAATGGGACGAGGCTATCATTATGCTACTTGTCTTGAAGGGGCACT
GAAAATCAAAGAAATTACTTATATGCACTCTGAAGGCATCCTTGCTGGTG
AATTGAAACATGGCCCTCTGGCTTTGGTGGATAAATTGATGCCTGTGATC
ATGATCATCATGAGAGATCACACTTATGCCAAGTGTCAGAATGCTCTTCA
GCAAGTGGTTGCTCGGCAGGGGCGGCCTGTGGTAATTTGTGATAAGGAGG
ATACTGAGACCATTAAGAACACAAAAAGAACGATCAAGGTGCCCCACTCA
GTGGACTGCTTGCAGGGCATTCTCAGCGTGATCCCTTTACAGTTGCTGGC
TTTCCACCTTGCTGTGCTGAGAGGCTATGATGTTGATTTCCCACGGAATC
TTGCCAAATCTGTGACTGTAGAGTGACATTTGCGGCCGC

Amino Acid Sequence

MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDW
EANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHG
EPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTE
TIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVG
TRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPV
EEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGD
HHHHHHHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRV
NFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTEL
PVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTV
GITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCD
DRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMG
RGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIM
RDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCL
QGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15827
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Internal 6His for baculovirus expression.; ; Estimated MW of expressed protein : 77579 Daltons