cDNA Clone NEK2

• Plasmid: pEBG6P-NEK2 D159A
• Parent Plasmid: pEBG6P
• Expressed: GST NEK2 D159A
• Genbank: Z29066
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTTCCCGGGCTGAGGACTATGAAGTGTTGTACACCATTGG
CACAGGCTCCTACGGCCGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCA
AGATATTAGTTTGGAAAGAACTTGACTATGGCTCCATGACAGAAGCTGAG
AAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGTGAACTGAAACATCC
AAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACAACAC
TGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATT
ACAAAGGGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCG
AGTGATGACTCAGTTGACTCTGGCCCTGAAGGAATGCCACAGACGAAGTG
ATGGTGGTCATACCGTATTGCATCGGGATCTGAAACCAGCCAATGTTTTC
CTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTTGGGCTAGCTAGAAT
ATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCTTATT
ACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGAT
ATCTGGTCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCC
ATTTACAGCTTTTAGCCAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCA
AATTCAGGCGAATTCCATACCGTTACTCTGATGAATTGAATGAAATTATT
ACGAGGATGTTAAACTTAAAGGATTACCATCGACCTTCTGTTGAAGAAAT
TCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGAAGAA
ATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGAT
TCCAGCCCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGA
GCGAGAGCGAGCTCTCAAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAAC
AGGAGCTTTGTGTTCGTGAGAGACTAGCAGAGGACAAACTGGCTAGAGCA
GAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAACGGAAGTTCCTGTC
TCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATTAAGA
AGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAG
AATTCTGAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAA
AAGGCTTCACGCTGCCCAGCTGCGGGCTCAAGCCCTGTCAGATATTGAGA
AAAATTACCAACTGAAAAGCAGACAGATCCTGGGCATGCGCTAGGCGGCC
GC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPSRAEDYEVLYT
IGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELK
HPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFV
LRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGAFGLA
RILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALM
PPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVE
EILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQL
QERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKF
LSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDL
KKRLHAAQLRAQALSDIEKNYQLKSRQILGMR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15821
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion for mammalian expression.; ; Estimated MW of expressed protein : 79135 Daltons