cDNA Clone KSR2

• Plasmid: pCMV-HA-2 KSR2
• Parent Plasmid: pCMV5 HA2
• Expressed: HA KSR2
• Genbank: AAQ24226.1
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGACGGATGAACAGGTGTGCGAGACTGTGGAGAAATACGG
AGCCAACCGGGAGGAGTGTGCCCGCCTCAACGCCTCCCTCTCCTGCCTCA
GGAATGTCCACATGTCAGGAGGCAACCTTTCCAAACAAGACTGGACCATC
CAGTGGCCCACCACAGAGACGGGGAAGGAGAACAATCCCGTGTGCCCCCC
GGAGCCCACCCCGTGGATCCGCACCCATCTCTCCCAGAGCCCCAGGGTCC
CGTCCAAGTGCGTCCAGCACTATTGTCACACCAGCCCCACTCCCGGGGCC
CCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCTACCCGGGCTT
GTGCCCGCCCCCGCCACTGGAGTCGGGCCACCGTTCCCTGCCCCCATCGC
CCCGGCAGCGGCACGCGGTCCGCACCCCGCCGCGCACCCCCAACATCGTC
ACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGAAGAACAAGCT
GAAGCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTGATACACTTGA
TCCCGGGATTCACCGCGCTGCATCGGAGCAAATCCCACGAGTTCCAGCTG
GGGCACCGCGTGGACGAGGCCCACACGCCCAAAGCCAAGAAGAAGAGCAA
ACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGCGAGAACATCC
CCTCTCAGCAGCGCTCCCCGCTGCTGTCCGAGCGCTCCCTCCGCTCCTTC
TTTGTGGGACACGCACCTTTCCTGCCTTCCACCCCTCCTGTTCACACTGA
GGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGCTGGTCCCCGCAGA
TCCCTCGCAGAGATCTTGGCAACTCCATCAAGCACAGGTTTTCCACCAAG
TACTGGATGTCTCAGACGTGCACAGTCTGTGGGAAAGGGATGCTTTTTGG
CCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCACAACAAATGCACCAAAG
AAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGATCCAGCAAGG
TTAGTCCGGACAGAGTCCGTTCCGTGTGACATCAACAACCCTCTACGGAA
GCCACCTCGCTATTCAGACCTGCACATCAGTCAGACGCTCCCCAAAACCA
ACAAAATCAACAAGGACCACATCCCTGTCCCTTACCAGCCAGACTCCAGC
AGCAACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGCCAGCACCCCC
CCTCCCTCCTAGTGCCACGCCGCCTTCTCCCCTACACCCTTCCCCACAGT
GCACACGGCAGCAGAAGAACTTCAACCTGCCAGCATCCCACTACTACAAA
TACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTGCCGGAGACGCC
GACCCGGGCGCCCCAGGTCATCCTGCATCCGGTGACCTCGAATCCAATCT
TGGAAGGAAATCCATTACTTCAAATTGAAGTGGAGCCAACGTCGGAGAAT
GAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGGATGACTTCGAGGAGAT
GAACCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAGGCCAGCCAGA
CCAGCATCTTCCTTCAGGAGTGGGACATCCCCTTTGAGCAGCTGGAGATC
GGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAGTGTACCACGGCCGCTG
GCATGGCGAGGTGGCCATCCGGCTGATTGACATTGAGAGGGACAACGAGG
ACCAGCTCAAGGCCTTCAAGCGGGAGGTGATGGCCTACAGGCAGACACGG
CATGAGAACGTGGTGCTTTTCATGGGTGCCTGCATGAGCCCGCCTCACCT
GGCCATCATCACCAGCCTCTGTAAGGGACGGACGCTCTATTCCGTTGTGA
GGGATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCAGATTGCTCAA
GAAATTGTGAAGGGCATGGGCTACCTCCACGCCAAGGGAATCCTACACAA
GGACCTCAAGTCAAAGAACGTCTTCTATGACAACGGCAAAGTGGTCATCA
CGGACTTTGGACTCTTCAGCATTTCTGGGGTGCTGCAGGCTGGCAGGCGG
GAGGACAAACTGCGCATCCAGAATGGCTGGCTATGCCACCTGGCACCAGA
GATCATCCGCCAGCTGTCCCCCGACACAGAGGAGGATAAGCTCCCCTTCT
CCAAGCACTCTGACGTCTTTGCCCTTGGCACAATCTGGTATGAACTCCAC
GCCAGGGAATGGCCTTTCAAGACCCAACCAGCAGAGGCAATAATCTGGCA
AATGGGCACAGGCATGAAACCCAACCTCAGCCAGATTGGCATGGGAAAAG
AAATCTCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACAAGAAGAGAGA
CCTACCTTCACCAAGCTCATGGACATGCTGGAGAAACTGCCAAAGCGAAA
CCGTCGCCTGTCTCACCCTGGACATTTCTGGAAGTCTGCAGAGCTGTGAG
CGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMTDEQVCETVEKYGANREECARLNASL
SCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQS
PRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSL
PPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKL
IHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSC
ENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRW
SPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNK
CTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTL
PKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHP
SPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTS
NPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRK
ASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIER
DNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLY
SVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGK
VVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDK
LPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIG
MGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSA
EL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15594
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: aa122-950 with respect to updated NCBI ref Q6VAB6.2; ; N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 95947 Daltons