cDNA Clone KSR2

• Plasmid: pCMV-FLAG-2 KSR2
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG KSR2
• Genbank: AAQ24226.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGACGGATGAACAGGTGTGCGAGACTGTGGAGAAATACGG
AGCCAACCGGGAGGAGTGTGCCCGCCTCAACGCCTCCCTCTCCTGCCTCA
GGAATGTCCACATGTCAGGAGGCAACCTTTCCAAACAAGACTGGACCATC
CAGTGGCCCACCACAGAGACGGGGAAGGAGAACAATCCCGTGTGCCCCCC
GGAGCCCACCCCGTGGATCCGCACCCATCTCTCCCAGAGCCCCAGGGTCC
CGTCCAAGTGCGTCCAGCACTATTGTCACACCAGCCCCACTCCCGGGGCC
CCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCTACCCGGGCTT
GTGCCCGCCCCCGCCACTGGAGTCGGGCCACCGTTCCCTGCCCCCATCGC
CCCGGCAGCGGCACGCGGTCCGCACCCCGCCGCGCACCCCCAACATCGTC
ACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGAAGAACAAGCT
GAAGCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTGATACACTTGA
TCCCGGGATTCACCGCGCTGCATCGGAGCAAATCCCACGAGTTCCAGCTG
GGGCACCGCGTGGACGAGGCCCACACGCCCAAAGCCAAGAAGAAGAGCAA
ACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGCGAGAACATCC
CCTCTCAGCAGCGCTCCCCGCTGCTGTCCGAGCGCTCCCTCCGCTCCTTC
TTTGTGGGACACGCACCTTTCCTGCCTTCCACCCCTCCTGTTCACACTGA
GGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGCTGGTCCCCGCAGA
TCCCTCGCAGAGATCTTGGCAACTCCATCAAGCACAGGTTTTCCACCAAG
TACTGGATGTCTCAGACGTGCACAGTCTGTGGGAAAGGGATGCTTTTTGG
CCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCACAACAAATGCACCAAAG
AAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGATCCAGCAAGG
TTAGTCCGGACAGAGTCCGTTCCGTGTGACATCAACAACCCTCTACGGAA
GCCACCTCGCTATTCAGACCTGCACATCAGTCAGACGCTCCCCAAAACCA
ACAAAATCAACAAGGACCACATCCCTGTCCCTTACCAGCCAGACTCCAGC
AGCAACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGCCAGCACCCCC
CCTCCCTCCTAGTGCCACGCCGCCTTCTCCCCTACACCCTTCCCCACAGT
GCACACGGCAGCAGAAGAACTTCAACCTGCCAGCATCCCACTACTACAAA
TACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTGCCGGAGACGCC
GACCCGGGCGCCCCAGGTCATCCTGCATCCGGTGACCTCGAATCCAATCT
TGGAAGGAAATCCATTACTTCAAATTGAAGTGGAGCCAACGTCGGAGAAT
GAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGGATGACTTCGAGGAGAT
GAACCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAGGCCAGCCAGA
CCAGCATCTTCCTTCAGGAGTGGGACATCCCCTTTGAGCAGCTGGAGATC
GGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAGTGTACCACGGCCGCTG
GCATGGCGAGGTGGCCATCCGGCTGATTGACATTGAGAGGGACAACGAGG
ACCAGCTCAAGGCCTTCAAGCGGGAGGTGATGGCCTACAGGCAGACACGG
CATGAGAACGTGGTGCTTTTCATGGGTGCCTGCATGAGCCCGCCTCACCT
GGCCATCATCACCAGCCTCTGTAAGGGACGGACGCTCTATTCCGTTGTGA
GGGATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCAGATTGCTCAA
GAAATTGTGAAGGGCATGGGCTACCTCCACGCCAAGGGAATCCTACACAA
GGACCTCAAGTCAAAGAACGTCTTCTATGACAACGGCAAAGTGGTCATCA
CGGACTTTGGACTCTTCAGCATTTCTGGGGTGCTGCAGGCTGGCAGGCGG
GAGGACAAACTGCGCATCCAGAATGGCTGGCTATGCCACCTGGCACCAGA
GATCATCCGCCAGCTGTCCCCCGACACAGAGGAGGATAAGCTCCCCTTCT
CCAAGCACTCTGACGTCTTTGCCCTTGGCACAATCTGGTATGAACTCCAC
GCCAGGGAATGGCCTTTCAAGACCCAACCAGCAGAGGCAATAATCTGGCA
AATGGGCACAGGCATGAAACCCAACCTCAGCCAGATTGGCATGGGAAAAG
AAATCTCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACAAGAAGAGAGA
CCTACCTTCACCAAGCTCATGGACATGCTGGAGAAACTGCCAAAGCGAAA
CCGTCGCCTGTCTCACCCTGGACATTTCTGGAAGTCTGCAGAGCTGTGAG
CGGCCGCG

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMTDEQVCETVEKYGANREECARLNASLS
CLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSP
RVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLP
PSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLI
HLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCE
NIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWS
PQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKC
TKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLP
KTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPS
PQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSN
PILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKA
SQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERD
NEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYS
VVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKV
VITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKL
PFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGM
GKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAE
L*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15593
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: aa122-950 with respect to updated NCBI ref Q6VAB6.2; ; N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 95858 Daltons