cDNA Clone IKK epsilon; ABIN2

• Plasmid: pFB Dual GST IKK epsilon, ABIN2
• Parent Plasmid: pFastbacDual
• Expressed: GST IKK epsilon untagged, ABIN2
• Genbank: NP_054721 and NP_077285.2
• Tag: untagged
• Species: Human

Sequence of Insert

DNA sequence of IKK epsilon insert :
AGATCTATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCA
ACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATT
TGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTG
GGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATT
AACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGT
TGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCG
GTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTT
TGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAA
TGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTA
ACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACAT
GGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAAC
GTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTAT
ATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCA
TCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCA
TGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAG
GGGGCCACTGCCAGTGTGTACAAGGCCCGCAACAAGAAATCCGGAGAGCT
GGTTGCTGTGAAGGTCTTCAACACTACCAGCTACCTGCGGCCCCGCGAGG
TGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCAGAACATC
GTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGAAGGTACT
GGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGCC
CTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCGC
TGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCG
CGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAGGGGCAGA
GCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGCTGGATGATGAT
GAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATCCCGACAT
GTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTGA
CTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGCCACTGGC
AGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCAT
GTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGTGCCCAGA
GGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCATCACCTGC
CAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATCCTGGCCAACAT
CCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCGG
AGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCC
CAGGCAGTCCTGCACCACATCTATATCCATGCCCACAACACGATAGCCAT
TTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGACACCAGG
AGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGTCTCAGCA
CAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCTTCAGCAC
AGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGACGTCCCCA
AGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGC
GTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGA
TGGGCAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAGGTGCTCC
AGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCTCCTCTAC
CTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTGGAACGCC
TGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGGCTGCGGA
CTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGAGACCCAG
GAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGT
ACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGACAAGATGA
ACTTCATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGGGCTTGGC
TACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCAGTCATTT
AGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTATC
AAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCACGAGACC
AGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTAACACAGA
AGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAGCTATCTC
ACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCCGCCTCCC
ATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCCACATGCA
AGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAACA
ACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGATGTCTGA
GCGGCCGC

DNA Sequence of Abin2 insert :
CCATGGGAAACCATGTCCCGGGACCCGGGGTCGGGCGGCTGGGAGGAGGC
CCCGCGCGCAGCTGCCGCGCTCTGCACCCTGTACCACGAGGCCGGACAGC
GGCTGCGCCGCCTGCAGGACCAGCTCGCTGCCCGCGACGCCCTCATCGCT
CGCCTCCGCGCCCGCCTGGCCGCGCTGGAGGGGGACGCCGCGCCGTCCCT
AGTGGACGCGCTGCTGGAGCAGGTTGCGCGCTTCCGGGAGCAGCTGCGAA
GGCAGGAGGGCGGCGCCGCCGAGGCCCAGATGCGCCAGGAAATTGAGAGG
CTGACTGAGCGACTAGAAGAAAAAGAGAGGGAGATGCAGCAGCTGCTGAG
CCAGCCCCAACACGAGCGAGAGAAGGAAGTCGTCCTGCTACGGAGGAGCA
TGGCAGAAGGGGAGCGCGCCCGGGCCGCCAGTGACGTCCTGTGCCGCTCC
TTGGCCAACGAGACCCATCAGCTGCGGAGGACGCTGACCGCCACCGCCCA
CATGTGTCAGCATCTGGCCAAGTGTCTGGATGAACGACAGCATGCACAAA
GGAATGTGGGGGAGAGAAGTCCTGACCAGTCGGAACACACAGATGGGCAC
ACCTCTGTCCAGAGTGTTATTGAGAAGTTGCAGGAAGAAAATCGACTGTT
AAAACAGAAGGTGACTCACGTTGAAGACCTCAATGCCAAGTGGCAGCGCT
ACAACGCCAGCAGGGACGAATACGTGAGGGGGCTCCATGCGCAGCTCAGG
GGGCTGCAGATCCCCCACGAGCCCGAGCTGATGAGGAAGGAGATCTCCCG
GCTCAACAGACAGTTGGAAGAGAAAATAAATGACTGTGCCGAAGTGAAGC
AGGAGCTGGCGGCCTCCAGGACGGCCCGGGATGCTGCGTTGGAGCGGGTG
CAGATGCTGGAACAGCAGATTCTCGCTTACAAGGATGACTTCATGTCAGA
AAGGGCCGATCGGGAACGGGCTCAAAGTAGGATTCAAGAACTGGAGGAAA
AGGTCGCCTCTTTGCTGCACCAGGTGTCCTGGAGACAGGATTCTCGAGAG
CCAGACGCCGGCCGGATTCACGCTGGGAGCAAAACTGCCAAGTATTTGGC
CGCCGACGCATTAGAGCTTATGGTGCCTGGTGGCTGGAGGCCTGGGACTG
GGTCCCAGCAGCCAGAACCCCCTGCAGAGGGCGGGCATCCTGGCGCGGTC
CAGAGAGGCCAGGGGGACCTTCAGTGCCCTCACTGCCTGCAGTGCTTCAG
TGACGAGCAAGGGGAAGAGCTCCTCAGGCATGTGGCCGAGTGCTGCCAGT
GAGCTAGC

Amino Acid Sequence

Protein sequence of IKK epsilon :
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTANYLWHTDDLLGQGA
TASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVK
LFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCV
VAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEK
FVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSL
PFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQL
SLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQA
VLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQH
IAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVL
GAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLS
SSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQES
LSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYN
EEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRN
HLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIA
PYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV*

Protein sequence of Abin2 :
MSRDPGSGGWEEAPRAAAALCTLYHEAGQRLRRLQDQLAARDALIARLRA
RLAALEGDAAPSLVDALLEQVARFREQLRRQEGGAAEAQMRQEIERLTER
LEEKEREMQQLLSQPQHEREKEVVLLRRSMAEGERARAASDVLCRSLANE
THQLRRTLTATAHMCQHLAKCLDERQHAQRNVGERSPDQSEHTDGHTSVQ
SVIEKLQEENRLLKQKVTHVEDLNAKWQRYNASRDEYVRGLHAQLRGLQI
PHEPELMRKEISRLNRQLEEKINDCAEVKQELAASRTARDAALERVQMLE
QQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREPDAG
RIHAGSKTAKYLAADALELMVPGGWRPGTGSQQPEPPAEGGHPGAVQRGQ
GDLQCPHCLQCFSDEQGEELLRHVAECCQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15026
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST tagged IKK epsilon and untagged Abin2 for baculovirus expression.; ; Estimated MW of expressed protein : 48724 Daltons