cDNA Clone Nemo

• Plasmid: pCMV5 Nemo FLAG
• Parent Plasmid: pCMV5
• Expressed: Nemo FLAG
• Genbank: AAH50612
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCCTTGCGGATCCGCCACCATGAATAGGCACCTCTGGAAGAGC
CAACTGTGTGAGATGGTGCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGA
CGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGC
CTTCAGAACAGGGCGCTCCTGAGACCCTCCAGCGCTGCCTGGAGGAGAAT
CAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCG
CTGCGAGGAGCTTCTGCATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGT
TCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGC
CTGGAGAAGCTCGATCTGAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGT
GGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTG
TGAAAGCCCAGGTGACGTCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGT
CGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCG
GGCGGCCAGCGAGCAGGCGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGC
AGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGC
GTGGAGGCCGCGCTCCGCATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAG
GAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACG
ACAACCACATCAAGAGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATG
CAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGT
GGCCAAACAGGAGGTGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACA
AGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTAC
AAGGCGGACTTCCAGGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAA
GAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCA
AACTGAAGGCCAGCTGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAG
CGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCT
CTCCTCTCCCCTGGCCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGC
CACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGAC
ACCCTGCAGATACATGTCATGGAGTGCATTGAGGACTACAAGGACGATGA
CGATAAGTAAGCGGCCGCCCAAGGGCGAATTC

Amino Acid Sequence

MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPET
LQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEA
RKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLL
GELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVD
QLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVV
GSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPV
LKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQES
ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKC
QYQAPDMDTLQIHVMECIEDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12932
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 49190 Daltons