cDNA Clone BAP1

• Plasmid: pGEX6P-1-BAP1
• Parent Plasmid: pGEX6P1
• Expressed: GST-BAP1
• Genbank: AAH01596.1
• Tag: GST

Sequence of Insert

GGATCCATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTT
CACCCTGCTCGTGGAAGATTTCGGTGTCAAGGGGGTGCAAGTGGAGGAGA
TCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTATCTTC
CTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTT
GGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCT
TTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGC
GTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCAT
GAAGGACTTCACCAAGGGTTTTAGCCCTGAGAGCAAAGGATATGCGATTG
GCAATGCCCCGGAGTTGGCCAAGGCCCATAATAGCCATGCCAGGCCCGAG
CCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGACCAT
GGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTG
AGCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAG
GACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGG
CCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGG
CAGTGGTGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTG
AAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCTGATAAGAGT
AACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGC
CTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCA
AACAGGGCCCCTGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGA
GGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAGCCCTCCCAACAAAC
CCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCC
AACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAA
TTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGG
GCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAG
GATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGC
CCTTAGGTATAAGGGGAAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTT
CTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACACCATCAACGTCTTG
GCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCAT
CAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACT
CGCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAG
ATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAA
CCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTT
TTGGAGAGGATGACAGCCTGCTGCGTGTTGACTGCATACGCTACAACCGT
GCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGC
TGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTT
CCTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCA
GTGGAGAAGGAGGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGAT
GGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAGGAGC
TGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCG
TGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGATTGATGACCA
GAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGC
TGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTG
CGGCGGCGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCC
TGACCGGCGGAAACGCTCTCGCCCCTACAAGGCCAAGCGCCAGTGAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNKGWLELESDPGLFTLLV
EDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDT
SVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFT
KGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFH
FVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATA
GEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPE
LIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGS
CAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKS
PMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYK
GKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSS
GAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRP
SSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGV
LSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPG
EPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTH
NYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRK
RSRPYKAKRQ

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12809
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 107182 Daltons