cDNA Clone IKK epsilon

• Plasmid: pFastbac GST IKK epsilon K38A
• Parent Plasmid: pFastbac GST
• Expressed: GST IKK epsilon K38A
• Genbank: NP_054721
• Tag: GST

Sequence of Insert

GGATCCATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCT
GGGGCAGGGGGCCACTGCCAGTGTGTACAAGGCCCGCAACAAGAAATCCG
GAGAGCTGGTTGCTGTGGCGGTCTTCAACACTACCAGCTACCTGCGGCCC
CGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCA
GAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGA
AGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTG
GAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGT
GCTGCGCTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTG
TGCATCGCGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAG
GGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGCTGGA
TGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATC
CCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTC
GGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGC
CACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGG
AGATCATGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGT
GCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCAT
CACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATCCTGG
CCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTC
TTTGCGGAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTC
CCTGTCCCAGGCAGTCCTGCACCACATCTATATCCATGCCCACAACACGA
TAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGA
CACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGT
CTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCT
TCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGAC
GTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGC
CAAGGGCGTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCC
TGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAG
GTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCT
CCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTG
GAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGG
CTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGA
GACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGG
ATCAGGTACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGAC
AAGATGAACTTCATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGG
GCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCA
GTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAG
AAGTATCAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCA
CGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTA
ACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAG
CTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCC
GCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCC
ACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTG
GACAACAACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGA
TGTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTANYLWHTDDLLGQGA
TASVYKARNKKSGELVAVAVFNTTSYLRPREVQVREFEVLRKLNHQNIVK
LFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCV
VAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEK
FVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSL
PFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQL
SLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQA
VLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQH
IAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVL
GAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLS
SSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQES
LSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYN
EEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRN
HLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIA
PYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12780
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression. Kinase dead mutation.; ; Estimated MW of expressed protein : 107225 Daltons