cDNA Clone Notch1 (mouse)

• Plasmid: pCIG (mouse) NICD
• Parent Plasmid: pCIG
• Genbank: NP_032740.2
• Species: Mouse

Sequence of Insert

GAATTCTAGCTAGCTAGCCGGCCCAATTCGGTACCATGGACTACAAAGAC
GATGACGATAAAGCCCGGCGGCAGCATGGCCAGCTCTGGTTCCCTGAGGG
TTTCAAAGTGTCAGAGGCCAGCAAGAAGAAGCGGAGAGAGCCCCTCGGCG
AGGACTCAGTCGGCCTCAAGCCCCTGAAGAATGCCTCAGATGGTGCTCTG
ATGGACGACAATCAGAACGAGTGGGGAGACGAAGACCTGGAGACCAAGAA
GTTCCGGTTTGAGGAGCCAGTAGTTCTCCCTGACCTGAGTGATCAGACTG
ACCACAGACAGTGGACCCAGCAGCACCTGGACGCTGCTGACCTGCGCATG
TCTGCCATGGCCCCAACACCGCCTCAGGGGGAGGTGGATGCTGACTGCAT
GGATGTCAATGTTCGAGGACCAGATGGCTTCACACCCCTCATGATTGCCT
CCTGCAGTGGAGGGGGCCTTGAGACAGGCAACAGTGAAGAAGAAGAAGAT
GCACCTGCTGTCATCTCTGACTTCATCTACCAGGGCGCCAGCTTGCACAA
CCAGACAGACCGCACCGGGGAGACCGCCTTGCACTTGGCTGCCCGATACT
CTCGTTCAGATGCTGCAAAGCGCTTGCTGGAGGCCAGTGCAGATGCCAAC
ATCCAGGACAACATGGGCCGTACTCCGTTACATGCAGCAGTTTCTGCAGA
TGCTCAGGGTGTCTTCCAGATCCTGCTCCGGAACAGGGCCACAGATCTGG
ATGCCCGAATGCATGATGGCACAACTCCACTGATCCTGGCTGCGCGCCTG
GCCCTGGAGGGCATGCTGGAGGACCTCATCAACTCACATGCTGACGTCAA
TGCCGTGGATGACCTAGGCAAGTCGGCTTTGCATTGGGCGGCCGCGGTGA
ACAATGTGGATGCTGCTGTTGTGCTCCTGAAGAACGGAGCCAACAAGGAC
ATGCAGAACAACAAGGAGGAGACTCCCCTGTTCCTGGCCGCCCGTGAGGG
CAGCTATGAGACTGCCAAAGTGTTGCTGGACCACTTTGCCAACCGGGACA
TCACGGATCACATGGACCGATTGCCGCGGGACATCGCACAGGAGCGTATG
CACCACGATATCGTGCGGCTTTTGGATGAGTACAACCTGGTGCGCAGCCC
ACAGCTGCATGGCACTGCCCTGGGTGGCACACCCACTCTGTCTCCCACAC
TCTGCTCGCCCAATGGCTACCTGGGCAATCTCAAGTCTGCCACACAGGGC
AAGAAGGCCCGCAAGCCCAGCACCAAAGGGCTGGCTTGTGGTAGCAAGGA
AGCTAAGGACCTCAAGGCACGGAGGAAGAAGTCCCAGGATGGCAAGGGCT
GCCTGTTGGACAGCTCGAGCATGCTGTCGCCTGTGGACTCCCTCGAGTCA
CCCCATGGCTACTTGTCAGATGTGGCCTCGCCACCCCTCCTCCCCTCCCC
ATTCCAGCAGTCTCCATCCATGCCTCTCAGCCACCTGCCTGGTATGCCTG
ACACTCACCTGGGCATCAGCCACTTGAATGTGGCAGCCAAGCCTGAGATG
GCAGCACTGGCTGGAGGTAGCCGGTTGGCCTTTGAGCCACCCCCGCCACG
CCTCTCCCACCTGCCTGTAGCCTCCAGTGCCAGCACAGTGCTGAGTACCA
ATGGCACGGGGGCTATGAATTTCACCGTGGGTGCACCGGCAAGCTTGAAT
GGCCAGTGTGAGTGGCTTCCCCGGCTCCAGAATGGCATGGTGCCCAGCCA
GTACAACCCACTACGGCCGGGTGTGACGCCGGGCACACTGAGCACACAGG
CAGCTGGCCTCCAGCATAGCATGATGGGGCCACTACACAGCAGCCTCTCC
ACCAATACCTTGTCCCCGATTATTTACCAGGGCCTGCCCAACACACGGCT
GGCAACACAGCCTCACCTGGTGCAGACCCAGCAGGTGCAGCCACAGAACT
TACAGCTCCAGCCTCAGAACCTGCAGCCACCATCACAGCCACACCTCAGT
GTGAGCTCGGCAGCCAATGGGCACCTGGGCCGGAGCTTCTTGAGTGGGGA
GCCCAGTCAGGCAGATGTACAACCGCTGGGCCCCAGCAGTCTGCCTGTGC
ACACCATTCTGCCCCAGGAAAGCCAGGCCCTGCCCACATCACTGCCATCC
TCCATGGTCCCACCCATGACCACTACCCAGTTCCTGACCCCTCCTTCCCA
GCACAGTTACTCCTCCTCCCCTGTGGACAACACCCCCAGCCACCAGCTGC
AGGTGCCAGAGCACCCCTTCCTCACCCCATCCCCTGAGTCCCCTGACCAG
TGGTCCAGCTCCTCCCCGCATTCCAACATCTCTGATTGGTCCGAGGGCAT
CTCCAGCCCGCCCACCACCATGCCGTCCCAGATCACCCACATTCCAGAGG
CATTTAAATAAACAGAGATGTGGGATGCAGGACCCCAGCTTCCGTTCCCA
AGCCCTGTTGGGAGTCCTTTCCAGTGCTTCAGGATGCTGGGGCGACCAAA
GGAGCCTTTTAAAAAATGTTTTTATACAAAATAAGAGGACAAGAATTTCC
ATTTTTTTTTTTAGTATTTATTTATGTACTTTTATTTTCCACAGAAACAC
TGCCTTTTTATTTATATGTATTGTTTTCTATGGCACTAGGGAAAAACATA
TCTGTTCCAAGAAAATAAACTAGGGGCCGGCTAGCTAGCTAGAATTC

Amino Acid Sequence

MDYKDDDDKARRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNA
SDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLSDQTDHRQWTQQHLDA
ADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNS
EEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEA
SADANIQDNMGRTPLHAAVSADAQGVFQILLRNRATDLDARMHDGTTPLI
LAARLALEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKN
GANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDI
AQERMHHDIVRLLDEYNLVRSPQLHGTALGGTPTLSPTLCSPNGYLGNLK
SATQGKKARKPSTKGLACGSKEAKDLKARRKKSQDGKGCLLDSSSMLSPV
DSLESPHGYLSDVASPPLLPSPFQQSPSMPLSHLPGMPDTHLGISHLNVA
AKPEMAALAGGSRLAFEPPPPRLSHLPVASSASTVLSTNGTGAMNFTVGA
PASLNGQCEWLPRLQNGMVPSQYNPLRPGVTPGTLSTQAAGLQHSMMGPL
HSSLSTNTLSPIIYQGLPNTRLATQPHLVQTQQVQPQNLQLQPQNLQPPS
QPHLSVSSAANGHLGRSFLSGEPSQADVQPLGPSSLPVHTILPQESQALP
TSLPSSMVPPMTTTQFLTPPSQHSYSSSPVDNTPSHQLQVPEHPFLTPSP
ESPDQWSSSSPHSNISDWSEGISSPPTTMPSQITHIPEAFK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU12486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Expression vector for Notch1 domain. Please note that the construct has only been sequenced from either end and the internal region is presumed based on the above noted genbank reference.; ; Estimated MW of expressed protein : 85160 Daltons