cDNA Clone OBP

• Plasmid: pGEX6P-1-OBP human
• Parent Plasmid: pGEX6P1
• Expressed: GST-OBP
• Genbank: NP_002547.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGACGGAGCTGAGAGGAGTGGTGGGGCCAGGCCCGGC
AGCCATTGCAGCACTTGGCGGCGGCGGCGCCGGTCCCCCAGTGGTGGGAG
GAGGCGGCGGCCGCGGAGATGCGGGGCCAGGCTCCGGGGCCGCGTCAGGG
ACGGTGGTCGCGGCGGCGGCGGGAGGCCCGGGCCCGGGGGCCGGGGGAGT
GGCGGCGGCTGGCCCGGCCCCTGCGCCGCCGACTGGGGGCTCGGGCGGCT
CGGGCGCTGGGGGTTCGGGCTCGGCTCGAGAGGGCTGGCTCTTCAAATGG
ACCAATTATATCAAAGGCTACCAGCGGCGATGGTTCGTGCTGAGCAACGG
GCTCCTGAGCTACTACAGATCAAAGGCAGAAATGAGACATACCTGCCGTG
GTACCATCAACCTCGCCACAGCCAACATCACCGTGGAGGACTCCTGCAAC
TTCATCATTTCCAATGGGGGTGCTCAGACCTACCATCTGAAAGCTAGTTC
AGAAGTTGAGCGGCAGCGCTGGGTGACGGCCCTGGAACTGGCCAAGGCCA
AAGCTGTGAAGATGCTGGCAGAGTCAGATGAATCAGGAGATGAAGAGTCT
GTCTCACAAACTGACAAGACTGAGCTGCAGAATACCCTTCGGACCCTCTC
TAGCAAAGTAGAGGACTTGAGCACGTGCAATGACTTGATAGCTAAGCATG
GCACAGCTCTGCAGCGTTCTCTCAGTGAGCTGGAGTCCCTGAAGTTGCCT
GCTGAGAGCAATGAAAAGATCAAACAGGTCAACGAACGAGCCACACTCTT
TAGGATAACATCCAATGCCATGATCAACGCCTGCAGAGATTTCCTCATGT
TAGCCCAGACCCATAGTAAAAAATGGCAAAAGTCACTACAGTATGAAAGA
GACCAGCGTATCCGACTGGAAGAAACCCTCGAGCAGCTGGCGAAGCAGCA
TAATCACCTGGAGAGGGCCTTCCGAGGAGCCACGGTGCTGCCGGCAAACA
CTCCTGGCAATGTGGGTTCTGGTAAAGATCAGTGCTGCTCTGGCAAAGGG
GACATGAGCGATGAAGATGATGAGAATGAATTTTTTGATGCACCTGAGAT
CATCACCATGCCTGAAAATTTGGGCCACAAACGTACTGGCAGCAATATCA
GTGGAGCCAGCAGTGACATCAGCCTTGATGAACAGTACAAGCATCAGCTG
GAGGAGACCAAAAAGGAAAAGAGAACCAGAATACCATACAAGCCAAACTA
TAGCCTCAATTTATGGAGCATCATGAAGAACTGCATTGGAAAAGAACTCT
CTAAGATCCCCATGCCGGTAAACTTTAATGAGCCCTTGTCCATGCTTCAG
CGCCTTACTGAAGATCTGGAATACCATGAGCTGTTAGACCGAGCTGCAAA
ATGTGAGAATTCTCTAGAACAGCTCTGTTATGTTGCAGCTTTCACCGTGT
CCTCCTACTCCACTACTGTCTTCCGCACCAGTAAGCCATTCAACCCACTG
CTTGGGGAGACCTTTGAGCTGGACCGATTAGAGGAGAATGGGTACCGATC
CCTCTGTGAACAGGTGAGTCATCATCCCCCTGCTGCTGCGCACCATGCTG
AGTCCAAAAATGGCTGGACATTGCGTCAGGAAATCAAAATCACCAGCAAG
TTTCGAGGCAAATACCTCTCCATTATGCCCCTCGGTACCATTCATTGTAT
TTTCCATGCAACTGGGCACCACTACACTTGGAAGAAAGTTACCACAACTG
TACACAACATTATTGTGGGCAAGTTGTGGATAGATCAGTCTGGCGAAATT
GATATTGTGAATCACAAGACAGGAGACAAGTGTAATCTTAAATTTGTTCC
TTATAGCTACTTCTCTCGGGATGTAGCAAGAAAGGTGACGGGGGAAGTGA
CAGATCCATCAGGAAAAGTCCACTTTGCTCTTCTGGGGACGTGGGATGAG
AAAATGGAATGTTTCAAAGTACAGCCAGTCATTGGGGAAAATGGGGGTGA
TGCTCGACAGAGAGGCCATGAAGCAGAGGAAAGCAGGGTCATGCTGTGGA
AAAGGAATCCTTTACCGAAGAATGCAGAAAACATGTACTACTTCTCAGAG
CTTGCTCTGACTCTCAATGCTTGGGAAAGTGGCACTGCCCCCACAGACAG
CCGGTTACGACCTGACCAGAGACTGATGGAAAATGGACGCTGGGATGAAG
CAAATGCGGAGAAGCAGCGCCTGGAGGAAAAACAAAGACTTTCCAGAAAG
AAGAGAGAAGCGGAAGCTATGAAAGCCACAGAGGATGGCACACCATATGA
TCCCTATAAGGCACTGTGGTTTGAGCGGAAGAAGGACCCTGTTACCAAGG
AGTTAACCCATATTTATAGGGGAGAATACTGGGAGTGTAAAGAAAAACAG
GACTGGAGCTCATGCCCGGACATTTTCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAATELRGVVGPGPAAIAA
LGGGGAGPPVVGGGGGRGDAGPGSGAASGTVVAAAAGGPGPGAGGVAAAG
PAPAPPTGGSGGSGAGGSGSAREGWLFKWTNYIKGYQRRWFVLSNGLLSY
YRSKAEMRHTCRGTINLATANITVEDSCNFIISNGGAQTYHLKASSEVER
QRWVTALELAKAKAVKMLAESDESGDEESVSQTDKTELQNTLRTLSSKVE
DLSTCNDLIAKHGTALQRSLSELESLKLPAESNEKIKQVNERATLFRITS
NAMINACRDFLMLAQTHSKKWQKSLQYERDQRIRLEETLEQLAKQHNHLE
RAFRGATVLPANTPGNVGSGKDQCCSGKGDMSDEDDENEFFDAPEIITMP
ENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRTRIPYKPNYSLNL
WSIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYHELLDRAAKCENS
LEQLCYVAAFTVSSYSTTVFRTSKPFNPLLGETFELDRLEENGYRSLCEQ
VSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLGTIHCIFHAT
GHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKFVPYSYF
SRDVARKVTGEVTDPSGKVHFALLGTWDEKMECFKVQPVIGENGGDARQR
GHEAEESRVMLWKRNPLPKNAENMYYFSELALTLNAWESGTAPTDSRLRP
DQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKA
LWFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12457
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 116240 Daltons