cDNA Clone Flagellin

• Plasmid: pET FLAG FLAGellin
• Parent Plasmid: pET28a original
• Genbank: AAU27422.1
• Species: Legionella pneumophila

Sequence of Insert

CCATGGACTACAAGGACGACGATGACAAGATGGCTCAAGTAATCAACACT
AATGTGGCGTCGCTCACAGCCCAACGTAATTTGGGTGTTTCGGGCAACAT
GATGCAAACATCGATCCAGCGTTTATCATCGGGATTAAGGATTAACAGTG
CCAAAGATGATGCAGCAGGACTCGCCATTTCTCAACGAATGACTGCACAA
ATTCGCGGGATGAACCAAGCCGTTAGAAATGCCAACGATGGTATTTCCCT
GGCTCAGGTTGCTGAAGGAGCAATGCAGGAAACAACCAATATTTTACAAC
GCATGCGTGAGCTTTCCGTTCAAGCTGCTAACTCAACCAATAACTCATCT
GACCGGTCTTCAATTCAAAGTGAAATTTCACAATTAAAAAGTGAATTGGA
GCGTATTGCTCAAAATACTGAATTCAATGGTCAAAGAATTCTCGATGGTT
CTTTCTCTGGCGCAAGCTTCCAGGTTGGGGCGAATTCAAACCAAACCATC
AATTTCAGTATCGGCAGCACAAAAGCTTCTTCTCTTGGTGGTATTGCCAC
GGCAACAGGAACAGAAGTAGCAGGTGCAGCAGCGGCAGATATTACTATCG
CAATTGGAGGAGGAGCAGCAACCAGTATTAACTCTTCTGCCAATTTTACC
GGGGCACTAAACGGACAAGATGCTACGTCAGCCTATGCCAAAGCAGCGGC
TATCAACGATGCCGGGATAGGAGGACTATCGGTTACAGCGTCTACCAGCG
GCACACAAGCAGTTGGCGCAATAGGTGGAACTGCAGGTGATACCTACAAC
TTAACGATTAATGGTGTTGCAATATACACTAACCTTAACGTAGCAACGGC
ATTAACCAACTCTGATCTTAGAGATGCCATCAATGGCGTCAGTAACCAAA
CTGGAGTTGTTGCATCACTTAACGGTGGTAATATGACACTCACTGCTGCT
GATGGCAGAAACATTACGGTAACAGAAAGCGGTACAGGTTTTACTGCAGG
TACTGACGGTTTAACTGTAACTGGTGGCGCATTTGATGGCGCGCTACGCG
GTACTTTATCAATCAGCGCCGTTGATACTATCGCGATTGGTGGTACTGTT
GCTAACATTGGTTTAAGTGCCAATATCTCTAAAGACACAGTAGGGATAGA
TTCACTGGATGTGAGCACAGCGTCTGGCGCTCAAACAGCGATTAAGAGAA
TTGATGCAGCACTCAATAGCGTAAACTCTAATCGGGCTAATATGGGTGCT
CTGCAAAATCGCTTTGAATCAACGATAGCCAACCTGCAAAACGTATCAGA
CAACTTATCAGCTGCTAGAAGTCGTATTCAAGATGCTGATTACGCTGCTG
AAATGGCTAGTTTGACCAAAAACCAAATTCTGCAACAAGCAGGTACAGCG
ATGTTGGCACAAGCTAATAGCCTACCGCAATCTGTATTATCTTTGTTAGG
TCGATAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKMAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSA
KDDAAGLAISQRMTAQIRGMNQAVRNANDGISLAQVAEGAMQETTNILQR
MRELSVQAANSTNNSSDRSSIQSEISQLKSELERIAQNTEFNGQRILDGS
FSGASFQVGANSNQTINFSIGSTKASSLGGIATATGTEVAGAAAADITIA
IGGGAATSINSSANFTGALNGQDATSAYAKAAAINDAGIGGLSVTASTSG
TQAVGAIGGTAGDTYNLTINGVAIYTNLNVATALTNSDLRDAINGVSNQT
GVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGAFDGALRG
TLSISAVDTIAIGGTVANIGLSANISKDTVGIDSLDVSTASGAQTAIKRI
DAALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAE
MASLTKNQILQQAGTAMLAQANSLPQSVLSLLGR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU12367
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for bacterial expression.; ; Estimated MW of expressed protein : 48966 Daltons