cDNA Clone LIMK1

• Plasmid: pGEX6P-3-LIMK1
• Parent Plasmid: pGEX6P3
• Expressed: GST-LIMK1
• Genbank: P53667
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTAT
GGGAGAGGAAGGAAGCGAGTTGCCCGTGTGTGCAAGCTGCGGCCAGAGGA
TCTATGATGGCCAGTACCTCCAGGCCCTGAACGCGGACTGGCACGCAGAC
TGCTTCAGGTGTTGTGACTGCAGTGCCTCCCTGTCGCACCAGTACTATGA
GAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTATGGCG
AGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTG
GCTGGGGAGCTGAAGTACCACCCCGAGTGTTTCATCTGCCTCACGTGTGG
GACCTTTATCGGTGACGGGGACACCTACACGCTGGTGGAGCACTCCAAGC
TGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCCCCGTCATCGAG
CAGATCCTGCCTGACTCCCCTGGCTCCCACCTGCCCCACACCGTCACCCT
GGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTCAGTCTCCA
TTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCACTCACACACC
GTCCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAA
TTCCATCCACGTCGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCC
GAAATGTGCCCCTGGACGAGATTGACCTGCTGATTCAGGAAACCAGCCGC
CTGCTCCAGCTGACCCTCGAGCATGACCCTCACGATACACTGGGCCACGG
GCTGGGGCCTGAGACCAGCCCCCTGAGCTCTCCGGCTTATACTCCCAGCG
GGGAGGCGGGCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGC
ATCGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAGCG
CAAGGACCTGGGTCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACC
GCATCTTCCGGCCGTCGGACCTCATCCACGGGGAGGTGCTGGGCAAGGGC
TGCTTCGGCCAGGCTATCAAGGTGACACACCGTGAGACAGGTGAGGTGAT
GGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAGGACGTTCC
TCAAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAG
TTCATCGGGGTGCTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTA
CATCAAGGGCGGCACGCTCCGGGGCATCATCAAGAGCATGGACAGCCAGT
ACCCATGGAGCCAGAGAGTGAGCTTTGCCAAGGACATCGCATCAGGGATG
GCCTACCTCCACTCCATGAACATCATCCACCGAGACCTCAACTCCCACAA
CTGCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTGACTTCGGGCTGG
CGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTC
AAGAAGCCAGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTG
GATGGCACCTGAGATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATG
TGTTCTCCTTTGGGATCGTCCTGTGCGAGATCATCGGGCGGGTGAACGCA
GACCCTGACTACCTGCCCCGCACCATGGACTTTGGCCTCAACGTGCGAGG
ATTCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTTCTTCCCCA
TCACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTG
AAGCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCT
GCCACTGGGCCCACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCT
ACCGGCGCGGCGAGAGCGGACTGCCTGCCCACCCTGAGGTCCCCGACTGA
GCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMRLTLLCCTWREE
RMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQY
YEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLT
CGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTV
TLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDV
KNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLG
HGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPAS
QRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGE
VMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFIT
EYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNS
HNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNP
YWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNV
RGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAG
HLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12338
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 100076 Daltons