cDNA Clone IRAK1

• Plasmid: pcDNA3.1 FLAG IRAK1 K239A
• Parent Plasmid: pcDNA3.1
• Expressed: FLAG IRAK1 K239A
• Genbank: AAC41949
• Tag: FLAG
• Species: Human

Sequence of Insert

ACTAGTGCCACCATGGACTACAAGGACGATGACGATAAGATGGCCGGGGG
GCCGGGCCCGGGGGAGCCCGCAGCCCCCGGCGCCCAGCACTTCTTGTACG
AGGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTG
GAGCCCGCCGACTGGTGCCAGTTCGCCGCCCTGATCGTGCGCGACCAGAC
CGAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGT
GGCCCTGGATCAACCGCAACGCCCGTGTGGCCGACCTCGTGCACATCCTC
ACGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCC
TCCCGCCCCGCTTCCGTCCCCAGGCACCACTGCCCCGAGGCCCAGCAGCA
TCCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCC
TCAGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTC
AGGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCAC
CGCCATCTCCAGCCCCTTCTTCTACCAAGCCAGGCCCAGAGAGCTCAGTG
TCCCTCCTGCAGGGAGCCCGCCCCTCTCCGTTTTGCTGGCCCCTCTGTGA
GATTTCCCGGGGCACCCACAACTTCTCGGAGGAGCTCAAGATCGGGGAGG
GTGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCT
GTGGCGAGGCTGAAGGAGAACGCTGACCTGGAGTGGACTGCAGTGAAGCA
GAGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACA
TTGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTG
TACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGAC
CCAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGG
GTACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATC
CATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACC
CAAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCA
GCCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACC
CTGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGA
CACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTC
AGAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTG
GTGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAG
CACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCA
TGCAGATCTACAAGAAGCACCTGGACCCCAGGCCCGGGCCCTGCCCACCT
GAGCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCG
GGCCAAAAGGAGGCCTCCTATGACCCAGGTGTACGAGAGGCTAGAGAAGC
TGCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTTGGAGGCCGCCAGCTGC
ATCCCCCCTTCCCCGCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGC
CCACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCA
GTGCCCAGGCAGCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAG
AGTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTT
GACTCCAAGCTGCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTC
CTCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCC
CGGCCCACAGCCGTGGAAGGACTGGCCCTTGGCAGCTCTGCATCATCGTC
GTCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCC
AGAAGCTGGCCCTGTACGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTG
TCGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCC
CGAAGAAAGTGATGAATTTCAGAGCTGACATATG

Amino Acid Sequence

MDYKDDDDKMAGGPGPGEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPAD
WCQFAALIVRDQTELRLCERSGQRTASVLWPWINRNARVADLVHILTHLQ
LLRARDIITAWHPPAPLPSPGTTAPRPSSIPAPAEAEAWSPRKLPSSAST
FLSPAFPGSQTHSGPELGLVPSPASLWPPPPSPAPSSTKPGPESSVSLLQ
GARPSPFCWPLCEISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVARL
KENADLEWTAVKQSFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFL
PNGSLEDRLHCQTQACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDI
KSSNVLLDERLTPKLGDFGLARFSRFAGSSPSQSSMVARTQTVRGTLAYL
PEEYIKTGRLAVDTDTFSFGVVVLETLAGQRAVKTHGARTKYLKDLVEEE
AEEAGVALRSTQSTLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPELGL
GLGQLACCCLHRRAKRRPPMTQVYERLEKLQAVVAGVPGHLEAASCIPPS
PQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDES
LGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTA
VEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSS
LPGLGLEQDRQGPEESDEFQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12331
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for selectable expression (G418) in mammalian cell lines.; ; Estimated MW of expressed protein : 77567 Daltons