cDNA Clone NEMO

• Plasmid: pACT2.6 NEMO
• Parent Plasmid: pACT2.6
• Expressed: GAL4 DNA AD nemo
• Genbank: AAH50612
• Tag: GAL4 DNA-AD
• Species: Human

Sequence of Insert

GGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCC
CAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTC
TGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAG
ACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCG
GCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCC
AAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAG
GCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAG
GCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGC
AGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTG
CTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGA
ATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGC
AGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTG
GACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGA
GCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGG
CCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTG
GTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCA
GCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATA
AGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCG
GTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAG
GCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGC
TGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAG
TCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGC
CCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCA
GCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAG
TGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGA
GTGCATTGAGTAGGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTCGAG

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYAS
LGGHMAMGGSNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHL
PSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKE
FLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKAS
VKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREAL
QQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEY
DNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQH
KIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYS
KLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEE
PPDFCCPKCQYQAPDMDTLQIHVMECIE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU12317
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Y2H construct.; ; Estimated MW of expressed protein : 65391 Daltons