cDNA Clone CERK

• Plasmid: pFBHTb CERK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His CERK
• Genbank: AAH67255
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTTCAGCGAGGTGCTGCACGGTCTGATTGGGAGGACGCAGAG
GAGCGCCGGGGTCGACCAGAACCACCCCCGGGCTGTGCTGGTCCCCAGTA
GCCTCCGGATTGGAATCATTCCCGCAGGGTCAACGGACTGCGTGTGTTAC
TCCACCGTGGGCACCAGCGACGCAGAAACCTCGGCGCTGCATATCGTTGT
TGGGGACTCGCTGGCCATGGATGTGTCCTCAGTCCACCACAACAGCACAC
TCCTTCGCTACTCCGTGTCCCTGCTGGGCTACGGCTTCTACGGGGACATC
ATCAAGGACAGTGAGAAGAAACGGTGGTTGGGTCTTGCCAGATACGACTT
TTCAGGTTTAAAGACCTTCCTCTCCCACCACTGCTATGAAGGGACAGTGT
CCTTCCTCCCTGCACAACACACGGTGGGATCTCCAAGGGATAGGAAGCCC
TGCCGGGCAGGATGCTTTGTTTGCAGGCAAAGCAAGCAGCAGCTGGAGGA
GGAGCAGAAGAAAGCACTGTATGGTTTGGAAGCTGCGGAGGACGTGGAGG
AGTGGCAAGTCGTCTGTGGGAAGTTTCTGGCCATCAATGCCACAAACATG
TCCTGTGCTTGTCGCCGGAGCCCCAGGGGCCTCTCCCCGGCTGCCCACTT
GGGAGACGGGTCTTCTGACCTCATCCTCATCCGGAAATGCTCCAGGTTCA
ATTTTCTGAGATTTCTCATCAGGCACACCAACCAGCAGGACCAGTTTGAC
TTCACTTTTGTTGAAGTTTATCGCGTCAAGAAATTCCAGTTTACGTCGAA
GCACATGGAGGATGAGGACAGCGACCTCAAGGAGGGGGGGAAGAAGCGCT
TTGGGCACATTTGCAGCAGCCACCCCTCCTGCTGCTGCACCGTCTCCAAC
AGCTCCTGGAACTGCGACGGGGAGGTCCTGCACAGCCCTGCCATCGAGGT
CAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCACGAGGAATTGAAGAGA
ATCCGAAGCCAGACTCACACAGCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMFSEVLHGLIGRTQRSAGVDQN
HPRAVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMD
VSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFL
SHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALY
GLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGSSDL
ILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMEDEDS
DLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHCQLV
RLFARGIEENPKPDSHS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12309
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 41151 Daltons