cDNA Clone CERK

• Plasmid: pGEX6P-1-CERK
• Parent Plasmid: pGEX6P1
• Expressed: GST-CERK
• Genbank: AAH67255
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCAGCGAGGTGCTGCACGGTCTGATTGGGAGGACGCAGAG
GAGCGCCGGGGTCGACCAGAACCACCCCCGGGCTGTGCTGGTCCCCAGTA
GCCTCCGGATTGGAATCATTCCCGCAGGGTCAACGGACTGCGTGTGTTAC
TCCACCGTGGGCACCAGCGACGCAGAAACCTCGGCGCTGCATATCGTTGT
TGGGGACTCGCTGGCCATGGATGTGTCCTCAGTCCACCACAACAGCACAC
TCCTTCGCTACTCCGTGTCCCTGCTGGGCTACGGCTTCTACGGGGACATC
ATCAAGGACAGTGAGAAGAAACGGTGGTTGGGTCTTGCCAGATACGACTT
TTCAGGTTTAAAGACCTTCCTCTCCCACCACTGCTATGAAGGGACAGTGT
CCTTCCTCCCTGCACAACACACGGTGGGATCTCCAAGGGATAGGAAGCCC
TGCCGGGCAGGATGCTTTGTTTGCAGGCAAAGCAAGCAGCAGCTGGAGGA
GGAGCAGAAGAAAGCACTGTATGGTTTGGAAGCTGCGGAGGACGTGGAGG
AGTGGCAAGTCGTCTGTGGGAAGTTTCTGGCCATCAATGCCACAAACATG
TCCTGTGCTTGTCGCCGGAGCCCCAGGGGCCTCTCCCCGGCTGCCCACTT
GGGAGACGGGTCTTCTGACCTCATCCTCATCCGGAAATGCTCCAGGTTCA
ATTTTCTGAGATTTCTCATCAGGCACACCAACCAGCAGGACCAGTTTGAC
TTCACTTTTGTTGAAGTTTATCGCGTCAAGAAATTCCAGTTTACGTCGAA
GCACATGGAGGATGAGGACAGCGACCTCAAGGAGGGGGGGAAGAAGCGCT
TTGGGCACATTTGCAGCAGCCACCCCTCCTGCTGCTGCACCGTCTCCAAC
AGCTCCTGGAACTGCGACGGGGAGGTCCTGCACAGCCCTGCCATCGAGGT
CAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCACGAGGAATTGAAGAGA
ATCCGAAGCCAGACTCACACAGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFSEVLHGLIGRTQRSAGV
DQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSL
AMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLK
TFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKK
ALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGS
SDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMED
EDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHC
QLVRLFARGIEENPKPDSHS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12308
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 64603 Daltons