cDNA Clone GYS1

• Plasmid: pPIC9 Glycogen Synth ase
• Parent Plasmid: pPIC9
• Expressed: alpha factor-Glycogen Synthase
• Genbank: AAH07688.1
• Tag: HA
• Species: Human

Sequence of Insert

CTCGAGAAAAGAGAGGCTGAAGCTATGCCTTTAAACCGCACTTTGTCCAT
GTCCTCACTGCCAGGACTGGAGGACTGGGAGGATGAATTCGACCTGGAGA
ACGCAGTGCTCTTCGAAGTGGCCTGGGAGGTGGCTAACAAGGTGGGTGGC
ATCTACACGGTGCTGCAGACGAAGGCGAAGGTGACAGGGGACGAATGGGG
CGACAACTACTTCCTGGTGGGGCCGTACACGGAGCAGGGCGTGAGGACCC
AGGTGGAACTGCTGGAGGCCCCCACCCCGGCCCTGAAGAGGACACTGGAT
TCCATGAACAGCAAGGGCTGCAAGGTGTATTTCGGGCGCTGGCTGATCGA
GGGAGGCCCTCTGGTGGTGCTCCTGGACGTGGGTGCCTCAGCTTGGGCCC
TGGAGCGCTGGAAGGGAGAGCTCTGGGATACCTGCAACATCGGAGTGCCG
TGGTACGACCGCGAGGCCAACGACGCTGTCCTCTTTGGCTTTCTGACCAC
CTGGTTCCTGGGTGAGTTCCTGGCACAGAGTGAGGAGAAGCCACATGTGG
TTGCTCACTTCCATGAGTGGTTGGCAGGCGTTGGACTCTGCCTGTGTCGT
GCCCGGCGACTGCCTGTAGCAACCATCTTCACCACCCATGCCACGCTGCT
GGGGCGCTACCTGTGTGCCGGTGCCGTGGACTTCTACAACAACCTGGAGA
ACTTCAACGTGGACAAGGAAGCAGGGGAGAGGCAGATCTACCACCGATAC
TGCATGGAAAGGGCGGCAGCCCACTGCGCTCACGTCTTCACTACTGTGTC
CCAGATCACCGCCATCGAGGCACAGCACTTGCTCAAGAGGAAACCAGATA
TTGTGACCCCCAATGGGCTGAATGTGAAGAAGTTTTCTGCCATGCATGAG
TTCCAGAACCTCCATGCTCAGAGCAAGGCTCGAATCCAGGAGTTTGTGCG
GGGCCATTTTTATGGGCATCTGGACTTCAACTTGGACAAGACCTTATACT
TCTTTATCGCCGGCCGCTATGAGTTCTCCAACAAGGGTGCTGACGTCTTC
CTGGAGGCATTGGCTCGGCTCAACTATCTGCTCAGAGTGAACGGCAGCGA
GCAGACAGTGGTTGCCTTCTTCATCATGCCAGCGCGGACCAACAATTTCA
ACGTGGAAACCCTCAAAGGCCAAGCTGTGCGCAAACAGCTTTGGGACACG
GCCAACACGGTGAAGGAAAAGTTCGGGAGGAAGCTTTATGAATCCTTACT
GGTTGGGAGCCTTCCCGACATGAACAAGATGCTGGATAAGGAAGACTTCA
CTATGATGAAGAGAGCCATCTTTGCAACGCAGCGGCAGTCTTTCCCCCCT
GTGTGCACCCACAATATGCTGGATGACTCCTCAGACCCCATCCTGACCAC
CATCCGCCGAATCGGCCTCTTCAATAGCAGTGCCGACAGGGTGAAGGTGA
TTTTCCACCCGGAGTTCCTCTCCTCCACAAGCCCCCTGCTCCCTGTGGAC
TATGAGGAGTTTGTCCGTGGCTGTCACCTTGGAGTCTTCCCCTCCTACTA
TGAGCCTTGGGGCTACACACCGGCTGAGTGCACGGTTATGGGAATCCCCA
GTATCTCCACCAACCTCTCCGGCTTCGGCTGCTTCATGGAGGAACACATC
GCAGACCCCTCAGCTTACGGTATCTACATTCTTGACCGGCGGTTCCGCAG
CCTGGATGATTCCTGCTCGCAGCTCACCTCCTTCCTCTACAGTTTCTGTC
AGCAGAGCCGGCGGCAGCGTATCATCCAGCGGAACCGCACGGAGCGCCTC
TCCGACCTTCTGGACTGGAAATACCTAGGCCGGTACTATATGTCTGCGCG
CCACATGGCGCTGTCCAAGGCCTTTCCAGAGCACTTCACCTACGAGCCCA
ACGAGGCGGATGCGGCCCAGGGGTACCGCTACCCACGGCCAGCCTCGGTG
CCACCGTCGCCCTCGCTGTCACGACACTCCAGCCCGCACCAGAGTGAGGA
TGAGGAGGATCCCCGGAACGGGCCGCTGGAGGAAGACGGCGAGCGCTACG
ATGAGGACGAGGAGGCCGCCAAGGACCGGCGCAACATCCGTGCACCAGAG
TGGCCGCGCCGAGCGTCCTGCACCTCCTCCACCAGCGGCAGCAAGCGCAA
CTCTGTGGACACGGCCACCTCCAGCTCACTCAGCACCCCGAGCGAGCCCC
TCAGCCCCACCAGCTCCCTGGGCGAGGAGCGTAACTAAGTCGAC

Amino Acid Sequence

MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDV
AVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEAMPLNRTLSMSS
LPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDN
YFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVYFGRWLIEGG
PLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTWF
LGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGR
YLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQI
TAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGH
FYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQT
VVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVG
SLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIR
RIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEP
WGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLD
DSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHM
ALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEE
DPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSV
DTATSSSLSTPSEPLSPTSSLGEERN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU12174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Pichia expression plasmid.