cDNA Clone JNK1

• Synonyms: MAPK8, SAPK1c
• Plasmid: pEBG6P-JNK1 L168A
• Parent Plasmid: pEBG6P
• Expressed: GST-JNK1 L168A D109E
• Genbank: ABK42246.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAGCAGAAGCAAGCGTGACAACAATTTTTATAGTGTAGAGAT
TGGAGATTCTACATTCACAGTCCTGAAACGATATCAGAATTTAAAACCTA
TAGGCTCAGGAGCTCAAGGAATAGTATGCGCAGCTTATGATGCCATTCTT
GAAAGAAATGTTGCAATCAAGAAGCTAAGCCGACCATTTCAGAATCAGAC
TCATGCCAAGCGGGCCTACAGAGAGCTAGTTCTTATGAAATGTGTTAATC
ACAAAAATATAATTGGCCTTTTGAATGTTTTCACACCACAGAAATCCCTA
GAAGAATTTCAAGATGTTTACATAGTCATGGAGCTCATGGATGCAAATCT
TTGCCAAGTGATTCAGATGGAGCTAGATCATGAAAGAATGTCCTACCTTC
TCTATCAGATGCTGTGTGGAATCAAGCACCTTCATTCTGCTGGAATTATT
CATCGGGACTTAAAGCCCAGTAATATAGTAGTAAAATCTGATTGCACTTT
GAAGATTGCTGACTTCGGTCTGGCCAGGACTGCAGGAACGAGTTTTATGA
TGACGCCTTATGTAGTGACTCGCTACTACAGAGCACCCGAGGTCATCCTT
GGCATGGGCTACAAGGAAAACGTGGATTTATGGTCCGTGGGGTGCATTAT
GGGAGAAATGGTTTGCCACAAAATCCTCTTTCCAGGAAGGGACTATATTG
ATCAGTGGAATAAAGTTATTGAACAGCTTGGAACACCATGTCCTGAATTC
ATGAAGAAACTGCAACCAACAGTAAGGACTTACGTTGAAAACAGACCTAA
ATATGCTGGATATAGCTTTGAGAAACTCTTCCCTGATGTCCTTTTCCCAG
CTGACTCAGAACACAACAAACTTAAAGCCAGTCAGGCAAGGGATTTGTTA
TCCAAAATGCTGGTAATAGATGCATCTAAAAGGATCTCTGTAGATGAAGC
TCTCCAACACCCGTACATCAATGTCTGGTATGATCCTTCTGAAGCAGAAG
CTCCACCACCAAAGATCCCTGACAAGCAGTTAGATGAAAGGGAACACACA
ATAGAAGAGTGGAAAGAATTGATATATAAGGAAGTTATGGACTTGGAGGA
GAGAACCAAGAATGGAGTTATACGGGGGCAGCCCTCTCCTTTAGCACAGG
TGCAGCAGTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSRSKRDNNFYSV
EIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQN
QTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDA
NLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDC
TLKIADFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGC
IMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENR
PKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVD
EALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDL
EERTKNGVIRGQPSPLAQVQQ

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 71603 Daltons