cDNA Clone BTK

• Plasmid: pFBHTb BTK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-BTK
• Genbank: NP_000052.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACA
GAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGA
CCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGAGAAGA
GGCAGTAAGAAGGGTTCAATAGATGTTGAGAAGATCACTTGTGTTGAAAC
AGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCGAGAAGAG
GTGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCT
TATCCCTTCCAGGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCC
AACTGAAGAACTAAGGAAGCGGTGGATTCACCAGCTCAAAAACGTAATCC
GGTACAACAGTGATCTGGTTCAGAAATATCACCCTTGCTTCTGGATCGAT
GGGCAGTATCTCTGCTGCTCTCAGACAGCCAAAAATGCTATGGGCTGCCA
AATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCTCACCGGA
AGACAAAAAAGCCTCTTCCCCCAACGCCTGAGGAGGACCAGATCTTGAAA
AAGCCACTACCGCCTGAGCCAGCAGCAGCACCAGTCTCCACAAGTGAGCT
GAAAAAGGTTGTGGCCCTTTATGATTACATGCCAATGAATGCAAATGATC
TACAGCTGCGGAAGGGTGATGAATATTTTATCTTGGAGGAAAGCAACTTA
CCATGGTGGAGAGCACGAGATAAAAATGGGCAGGAAGGCTACATTCCTAG
TAACTATGTCACTGAAGCAGAAGACTCCATAGAAATGTATGAGTGGTATT
CCAAACACATGACTCGGAGTCAGGCTGAGCAACTGCTAAAGCAAGAGGGG
AAAGAAGGAGGTTTCATTGTCAGAGACTCCAGCAAAGCTGGCAAATATAC
AGTGTCTGTGTTTGCTAAATCCACAGGGGACCCTCAAGGGGTGATACGTC
ATTATGTTGTGTGTTCCACACCTCAGAGCCAGTATTACCTGGCTGAGAAG
CACCTTTTCAGCACCATCCCTGAGCTCATTAACTACCATCAGCACAACTC
TGCAGGACTCATATCCAGGCTCAAATATCCAGTGTCTCAACAAAACAAGA
ATGCACCTTCCACTGCAGGCCTGGGATACGGATCATGGGAAATTGATCCA
AAGGACCTGACCTTCTTGAAGGAGCTGGGGACTGGACAATTTGGGGTAGT
GAAGTATGGGAAATGGAGAGGCCAGTACGACGTGGCCATCAAGATGATCA
AAGAAGGCTCCATGTCTGAAGATGAATTCATTGAAGAAGCCAAAGTCATG
ATGAATCTTTCCCATGAGAAGCTGGTGCAGTTGTATGGCGTCTGCACCAA
GCAGCGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCC
TGAACTACCTGAGGGAGATGCGCCACCGCTTCCAGACTCAGCAGCTGCTA
GAGATGTGCAAGGATGTCTGTGAAGCCATGGAATACCTGGAGTCAAAGCA
GTTCCTTCACCGAGACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAG
GAGTTGTTAAAGTATCTGATTTCGGCCTGTCCAGGTATGTCCTGGATGAT
GAATACACAAGCTCAGTAGGCTCCAAATTTCCAGTCCGGTGGTCCCCACC
GGAAGTCCTGATGTATAGCAAGTTCAGCAGCAAATCTGACATTTGGGCTT
TTGGGGTTTTGATGTGGGAAATTTACTCCCTGGGGAAGATGCCATATGAG
AGATTTACTAACAGTGAGACTGCTGAACACATTGCCCAAGGCCTACGTCT
CTACAGGCCTCATCTGGCTTCAGAGAAGGTATATACCATCATGTACAGTT
GCTGGCATGAGAAAGCAGATGAGCGTCCCACTTTCAAAATTCTTCTGAGC
AATATTCTAGATGTCATGGATGAAGAATCCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSAAVILESIFLKRSQQKKKTSPL
NFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNP
PPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKR
WIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNG
SLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALY
DYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAE
DSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKS
TGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRL
KYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRG
QYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFII
TEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLA
ARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSK
FSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLAS
EKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12110
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; ; Estimated MW of expressed protein : 79519 Daltons