cDNA Clone IKK beta

• Plasmid: pEBG6P-IKK beta
• Parent Plasmid: pEBG6P
• Expressed: GST-IKK beta
• Genbank: AF080158 (gi 14738764)
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGC
CTGGGAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCC
GATGGCACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGG
CAGGAGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGAT
CATGAGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTG
AGGGGATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAG
TACTGCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTG
CTGTGGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCT
CTGCGCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAG
CCAGAAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAAT
TATTGACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACAT
CATTCGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAG
AAGTACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGA
GTGCATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGT
GGCATTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAA
GACTTGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAA
TCTTAACAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTGATGC
TGATGTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAAT
GGCTGCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATAT
CTTGAACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATG
AGAGTCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCA
GAGGAGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGA
TAAGCCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACA
CATTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTAT
GAGACTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCT
TCAAGAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGG
GCCAGGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTG
CAGCAGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTG
CCTCTCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGG
CCAAGTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTAC
AGCGAGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTG
GAGGGAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGA
AACTCCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTA
CAGAGGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGA
GGAGCAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACC
AGCGAACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCA
ATTCAGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAA
AACTGTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAG
AGGTGGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAG
GAGAAGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAA
GGTCCGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGAC
TTAGCCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGC
TTACCTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAA
CCTCTGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAG
ACCAGAGTTTCACGGCCCTAGACTGGAGCTGGTTACAGACGGAAGAAGAA
GAGCACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGACCCCC
TGACATGGGGCAGCCCATAGCAGGCCTTGTGCGGTGGGGGGACTCGACCC
CCTGACATGGGGCTGCCTGGAGCAGGCCTCATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMSWS
PSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRN
RERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDL
RKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQ
QGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVD
YWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVK
FSSSLPYPNNLNSVLSERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALD
DILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELL
QEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPR
PQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAA
MMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFG
ITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGR
KQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKK
VRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKEL
WNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKK
SEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQ
AS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression. Protein differs at A270S from genbank (gi 14738764 revised) ; ; Estimated MW of expressed protein : 117233 Daltons