cDNA Clone IRF7

• Plasmid: pEBG6P-IRF7
• Parent Plasmid: pEBG6P
• Expressed: GST-IRF7
• Genbank: NP_001563
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCTTGGCTCCTGAGAGGGCAGCCCCACGCGTGCTGTTCGG
AGAGTGGCTCCTTGGAGAGATCAGCAGCGGCTGCTATGAGGGGCTGCAGT
GGCTGGACGAGGCCCGCACCTGTTTCCGCGTGCCCTGGAAGCACTTCGCG
CGCAAGGACCTGAGCGAGGCCGACGCGCGCATCTTCAAGGCCTGGGCTGT
GGCCCGCGGCAGGTGGCCGCCTAGCAGCAGGGGAGGTGGCCCGCCCCCCG
AGGCTGAGACTGCGGAGCGCGCCGGCTGGAAAACCAACTTCCGCTGCGCA
CTGCGCAGCACGCGTCGCTTCGTGATGCTGCGAGATAACTCGGGGGACCC
GGCCGACCCGCACAAGGTGTACGCGCTCAGCCGGGAGCTGTGCTGGCGAG
AAGGCCCAGGCACGGACCAGACTGAGGCAGAGGCCCCCGCAGCTGTCCCA
CCACCACAGGGTGGGCCCCCAGGGCCATTCCTGGCACACACACATGCTGG
ACTCCAAGCCCCAGGCCCCCTCCCTGCCCCAGCTGGTGACAAGGGGGACC
TCCTGCTCCAGGCAGTGCAACAGAGCTGCCTGGCAGACCATCTGCTGACA
GCGTCATGGGGGGCAGATCCAGTCCCAACCAAGGCTCCTGGAGAGGGACA
AGAAGGGCTTCCCCTGACTGGGGCCTGTGCTGGAGGCCCAGGGCTCCCTG
CTGGGGAGCTGTACGGGTGGGCAGTAGAGACGACCCCCAGCCCCGGGCCC
CAGCCCGCGGCACTAACGACAGGCGAGGCCGCGGCCCCAGAGTCCCCGCA
CCAGGCAGAGCCGTACCTGTCACCCTCCCCAAGCGCCTGCACCGCGGTGC
AAGAGCCCAGCCCAGGGGCGCTGGACGTGACCATCATGTACAAGGGCCGC
ACGGTGCTGCAGAAGGTGGTGGGACACCCGAGCTGCACGTTCCTATACGG
CCCCCCAGACCCAGCTGTCCGGGCCACAGACCCCCAGCAGGTAGCATTCC
CCAGCCCTGCCGAGCTCCCGGACCAGAAGCAGCTGCGCTACACGGAGGAA
CTGCTGCGGCACGTGGCCCCTGGGTTGCACCTGGAGCTTCGGGGGCCACA
GCTGTGGGCCCGGCGCATGGGCAAGTGCAAGGTGTACTGGGAGGTGGGCG
GACCCCCAGGCTCCGCCAGCCCCTCCACCCCAGCCTGCCTGCTGCCTCGG
AACTGTGACACCCCCATCTTCGACTTCAGAGTCTTCTTCCAAGAGCTGGT
GGAATTCCGGGCACGGCAGCGCCGTGGCTCCCCACGCTATACCATCTACC
TGGGCTTCGGGCAGGACCTGTCAGCTGGGAGGCCCAAGGAGAAGAGCCTG
GTCCTGGTGAAGCTGGAACCCTGGCTGTGCCGAGTGCACCTAGAGGGCAC
GCAGCGTGAGGGTGTGTCTTCCCTGGATAGCAGCAGCCTCAGCCTCTGCC
TGTCCAGCGCCAACAGCCTCTATGACGACATCGAGTGCTTCCTTATGGAG
CTGGAGCAGCCCGCCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMALAPERAAPRVL
FGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAW
AVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSG
DPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTH
AGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGE
GQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPES
PHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFL
YGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRG
PQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQE
LVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLE
GTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 81643 Daltons