cDNA Clone OPTINEURIN

• Plasmid: pEBG6P-Optineurin M98K
• Parent Plasmid: pEBG6P
• Expressed: GST-Optineurin M98K
• Genbank: NP_001008212
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAAAG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTC
CCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTG
AGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCA
GCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTA
GGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGT
CCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAG
GAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAA
CTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAG
AGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGA
AAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCA
CAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAA
GTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGA
GGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAG
AAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTG
AATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGT
GGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATG
AAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAA
GAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTC
AGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAAC
TGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAG
CAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAG
GGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGA
GAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTT
CTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGAT
GGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGG
CTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGG
AATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACAT
AGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSHQPLSCLTEKE
DSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQ
AMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLKALSHENEKLKEELGK
LKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSE
LQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSK
YRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSD
FEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKEL
QEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLEL
QVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRK
ESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTI
LRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQS
LMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLP
DIDTLQIHVMDCII*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 93334 Daltons