cDNA Clone NEMO

• Plasmid: pCMV-TAG3b NEMO E315A
• Parent Plasmid: pCMV tag3b
• Expressed: Myc-NEMO E315A
• Genbank: AAH50612
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCC
CAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTC
TGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAG
ACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCG
GCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCC
AAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAG
GCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAG
GCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGC
AGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTG
CTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGA
ATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGC
AGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTG
GACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGA
GCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGG
CCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTG
GTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCA
GCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATA
AGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCG
GTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGCGAG
GCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGC
TGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAG
TCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGC
CCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCA
GCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAG
TGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGA
GTGCATTGAGTAGGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTCGAG

Amino Acid Sequence

MEQKLISEEDLSPGGSNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGK
PAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQAS
QREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQM
AEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLE
SEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYH
QLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLK
EEAEQHKIVMETVPVLKAQADIYKADFQAARQAREKLAEKKELLQEQLEQ
LQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQR
RSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8771
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal Myc tag for mammalian expression.; ; Estimated MW of expressed protein : 49707 Daltons