cDNA Clone DDB1

• Plasmid: pEBG6P-DDB1
• Parent Plasmid: pEBG6P
• Expressed: GST-DDB1
• Genbank: NP_001914.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCGTACAACTACGTGGTAACGGCCCAGAAGCCCACCGC
CGTGAACGGCTGCGTGACCGGACACTTTACTTCGGCCGAAGACTTAAACC
TGTTGATTGCCAAAAACACGAGATTAGAGATCTATGTGGTCACCGCCGAG
GGGCTTCGGCCCGTCAAAGAGGTGGGCATGTATGGGAAGATTGCGGTCAT
GGAGCTTTTCAGGCCCAAGGGGGAGAGCAAGGACCTGCTGTTTATCTTGA
CAGCGAAGTACAATGCCTGCATCCTGGAGTATAAACAGAGTGGCGAGAGC
ATTGACATCATTACGCGAGCCCATGGCAATGTCCAGGACCGCATTGGCCG
CCCCTCAGAGACCGGCATTATTGGCATCATTGACCCTGAGTGCCGGATGA
TTGGCCTGCGTCTCTATGATGGCCTTTTCAAGGTTATTCCACTAGATCGC
GATAATAAAGAACTCAAGGCCTTCAACATCCGCCTGGAGGAGCTGCATGT
CATTGATGTCAAGTTCCTATATGGTTGCCAAGCACCTACTATTTGCTTTG
TCTACCAGGACCCTCAGGGGCGGCACGTAAAAACCTATGAGGTGTCTCTC
CGAGAAAAGGAATTCAATAAGGGCCCTTGGAAACAGGAAAATGTCGAAGC
TGAAGCTTCCATGGTGATCGCAGTCCCAGAGCCCTTTGGGGGGGCCATCA
TCATTGGACAGGAGTCAATCACCTATCACAATGGTGACAAATACCTGGCT
ATTGCCCCTCCTATCATCAAGCAAAGCACGATTGTGTGCCACAATCGAGT
GGACCCTAATGGCTCAAGATACCTGCTGGGAGACATGGAAGGCCGGCTCT
TCATGCTGCTTTTGGAGAAGGAGGAACAGATGGATGGCACCGTCACTCTC
AAGGATCTCCGTGTAGAACTCCTTGGAGAGACCTCTATTGCTGAGTGCTT
GACATACCTTGATAATGGTGTTGTGTTTGTCGGGTCTCGCCTGGGTGACT
CCCAGCTTGTGAAGCTCAACGTTGACAGTAATGAACAAGGCTCCTATGTA
GTGGCCATGGAAACCTTTACCAACTTAGGACCCATTGTCGATATGTGCGT
GGTGGACCTGGAGAGGCAGGGGCAGGGGCAGCTGGTCACTTGCTCTGGGG
CTTTCAAGGAAGGTTCTTTGCGGATCATCCGGAATGGAATTGGAATCCAC
GAGCATGCCAGCATTGACTTACCAGGCATCAAAGGATTATGGCCACTGCG
GTCTGACCCTAATCGTGAGACTTATGACACTTTGGTGCTCTCTTTTGTGG
GCCAGACAAGAGTTCTCATGTTAAATGGAGAGGAGGTAGAAGAAACCGAA
CTGATGGGTTTCGTGGATGATCAGCAGACTTTCTTCTGTGGCAACGTGGC
TCATCAGCAGCTTATCCAGATCACTTCAGCATCGGTGAGGTTGGTCTCTC
AAGAACCCAAAGCTCTGGTCAGTGAATGGAAGGAGCCTCAGGCCAAGAAC
ATCAGTGTGGCCTCCTGCAATAGCAGCCAGGTGGTGGTGGCTGTAGGCAG
GGCCCTCTACTATCTGCAGATCCATCCTCAGGAGCTCCGGCAGATCAGCC
ACACAGAGATGGAACATGAAGTGGCTTGCTTGGACATCACCCCATTAGGA
GACAGCAATGGACTGTCCCCTCTTTGTGCCATTGGCCTCTGGACGGACAT
CTCGGCTCGTATCTTGAAGTTGCCCTCTTTTGAACTACTGCACAAGGAGA
TGCTGGGTGGAGAGATCATTCCTCGCTCCATCCTGATGACCACCTTTGAG
AGTAGCCATTACCTCCTTTGTGCCTTGGGAGATGGAGCGCTTTTCTACTT
TGGGCTCAACATTGAGACAGGTCTGTTGAGCGACCGTAAGAAGGTGACTT
TGGGCACCCAGCCCACCGTATTGAGGACTTTTCGTTCTCTTTCTACCACC
AACGTCTTTGCTTGTTCTGACCGCCCCACTGTCATCTATAGCAGCAACCA
CAAATTGGTCTTCTCAAATGTCAACCTCAAGGAAGTGAACTACATGTGTC
CCCTCAATTCAGATGGCTATCCTGACAGCCTGGCGCTGGCCAACAATAGC
ACCCTCACCATTGGCACCATCGATGAGATCCAGAAGCTGCACATTCGCAC
AGTTCCCCTCTATGAGTCTCCAAGGAAGATCTGCTACCAGGAAGTGTCCC
AGTGTTTCGGGGTCCTCTCCAGCCGCATTGAAGTCCAAGACACGAGTGGG
GGCACGACAGCCTTGAGGCCCAGCGCTAGCACCCAGGCTCTGTCCAGCAG
TGTAAGCTCCAGCAAGCTGTTCTCCAGCAGCACTGCTCCTCATGAGACCT
CCTTTGGAGAAGAGGTGGAGGTGCATAACCTACTTATCATTGACCAACAC
ACCTTTGAAGTGCTTCATGCCCACCAGTTTCTGCAGAATGAATATGCCCT
CAGTCTGGTTTCCTGCAAGCTGGGCAAAGACCCCAACACTTACTTCATTG
TGGGCACAGCAATGGTGTATCCTGAAGAGGCAGAGCCCAAGCAGGGTCGC
ATTGTGGTCTTTCAGTATTCGGATGGAAAACTACAGACTGTGGCTGAAAA
GGAAGTGAAAGGGGCCGTGTACTCTATGGTGGAATTTAACGGGAAGCTGT
TAGCCAGCATCAATAGCACGGTGCGGCTCTATGAGTGGACAACAGAGAAG
GACGTGCGCACTGAGTGCAACCACTACAACAACATCATGGCCCTCTACCT
GAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTTATGCGCTCAGTGC
TGCTGCTTGCCTACAAGCCCATGGAAGGAAACTTTGAAGAGATTGCTCGA
GACTTTAATCCCAACTGGATGAGTGCTGTGGAAATCTTGGATGATGACAA
TTTTCTGGGGGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAAAAGGATA
GCGCTGCCACCACTGACGAGGAGCGGCAGCACCTCCAGGAGGTTGGTCTT
TTCCACCTGGGCGAGTTTGTCAATGTCTTTTGCCACGGCTCTCTGGTAAT
GCAGAATCTGGGTGAGACTTCCACCCCCACACAAGGCTCGGTGCTCTTCG
GCACGGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCAGAGAGCTGG
TACAACCTCCTGCTGGACATGCAGAATCGACTCAATAAAGTCATCAAAAG
TGTGGGGAAGATCGAGCACTCCTTCTGGAGATCCTTTCACACCGAGCGGA
AGACAGAACCAGCCACAGGTTTCATCGACGGTGACTTGATTGAGAGTTTC
CTGGATATTAGCCGCCCCAAGATGCAGGAGGTGGTGGCAAACCTACAGTA
TGACGATGGCAGCGGTATGAAGCGAGAGGCCACTGCAGACGACCTCATCA
AGGTTGTGGAGGAGCTAACTCGGATCCATTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMSYN
YVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKE
VGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRA
HGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKA
FNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNK
GPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIK
QSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVEL
LGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFT
NLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDL
PGIKGLWPLRSDPNRETYDTLVLSFVGQTRVLMLNGEEVEETELMGFVDD
QQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCN
SSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSP
LCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLC
ALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSD
RPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTI
DEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRP
SASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHA
HQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYS
DGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKDVRTECN
HYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWM
SAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFV
NVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDM
QNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPK
MQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pEBG6P-DDB161.19 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU8754
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 155189 Daltons
• Low Temp: No