cDNA Clone IKK beta

• Plasmid: pCMV-HA IKK beta
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-IKK beta
• Genbank: AF080158 (gi 14738764)
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGCCTGG
GAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCCGATG
GCACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGGCAGG
AGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCCAGATCATG
AGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCTGAGGG
GATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAGTACT
GCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTGCTGT
GGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATTGCCTCTGC
GCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCTAAAGCCAG
AAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACAAAATTATT
GACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACATCATT
CGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAGAAGT
ACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGAGTGC
ATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTGCAGTGGCA
TTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAGCGAAGACT
TGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCAATAATCTT
AACAGTGTCCTGGCTGAGCGACTGGAGAAGTGGCTGCAACTGATGCTGAT
GTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAATGGCT
GCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATATCTTG
AACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAGGATGAGAG
TCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCATCCCAGAGG
AGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCCCCGATAAG
CCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCACACATT
GGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTATGAGA
CTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCTTCAA
GAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTGTGGGGCCA
GGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCGGCTGCAGC
AGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACAGCTGCCTC
TCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAGGCCAA
GTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTACAGCG
AGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTGGAGG
GAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAAGTGAAACT
CCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGACTTACAGA
GGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACCTAGAGGAG
CAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGACCAGCG
AACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCAATTC
AGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAAAACT
GTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTGGAAGAGGT
GGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCTGCAGGAGA
AGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTAGCAAGGTC
CGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGACTTAG
CCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGCTTAC
CTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAACCTC
TGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAACAAGACCA
GAGTTTCACGGCCCTAGACTGGAGCTGGTTACAGACGGAAGAAGAAGAGC
ACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGACCCCCTGAC
ATGGGGCAGCCCATAGCAGGCCTTGTGCGGTGGGGGGACTCGACCCCCTG
ACATGGGGCTGCCTGGAGCAGGCCTCATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDSSGRSWSPSLTTQTCGAWEMKERLGTGGFGN
VIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARD
VPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSD
IASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSL
CTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQP
VQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWLQ
LMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVT
EDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNE
GHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRK
VWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQ
LKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGREN
EVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKP
RDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPK
VEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNA
SRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVR
EQDQSFTALDWSWLQTEEEEHSCLEQAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8533
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; ; Estimated MW of expressed protein : 89046 Daltons