cDNA Clone NOD2 (mouse)

• Plasmid: pEBG6P-NOD2 (mouse) D286N
• Parent Plasmid: pEBG6P
• Expressed: GST-NOD2 (mouse) D286N
• Genbank: AAH44774
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGTGCTCACAGGAAGAGTTCCAGGCACAGAGGAGCCAGCTGGT
GGCACTGCTGATCTCAGGGTCCTTGGAGGGCTTTGAGAGCATCTTAGACT
GGCTGCTGTCTTGGGATGTGCTCTCCAGGGAGGACTATGAGGGCCTCAGC
CTCCCTGGCCAACCTCTCTCCCATTCTGCCAGGCGTCTGCTGGACACAGT
CTGGAACAAGGGTGTTTGGGGCTGTCAGAAGCTCCTCGAAGCTGTGCAGG
AGGCACAGGCCAACAGTCATACCTTTGAACTGTATGGGTCCTGGGACACT
CACTCCCTTCATCCAACCAGAGACCTGCAGAGTCACCGGCCAGCCATTGT
GAGGAGACTCTACAACCATGTAGAAGCCATGCTGGAGCTGGCAAGGGAGG
GGGGGTTTCTGAGCCAGTACGAGTGTGAGGAGATCAGGCTGCCAATCTTC
ACGTCGTCTCAGAGGGCAAGAAGGCTGCTTGATCTCGCTGCGGTGAAGGC
CAATGGACTGGCTGCCTTCCTTCTACAGCACGTCAGGGAACTGCCAGCTC
CACTGCCTCTGCCTTACGAGGGCTACTCTCTGTGTAGGTCCCGCTGTGAC
CGTGGCTTCACCCTCATTTGCCTCTTCTGTATCCTAGCTGCTGAGTGTCA
GAAGTTCATATCTAAGCTGAGGACCATGGTGTTGGCTCAGTCTCGCTTCC
TCAGTACTTACGATGGGTCAGAGAATCTTTGCCTGGAGGATATATACACG
GAGAACATCTTGGAGCTGCGGACTGAAGTGGGCACAGCCGGGGCCTTGCA
GAAGAGCCCTGCCATCCTGGGCCTGGAGGACCTCTTTGATACCCATGGTC
ACCTGAACAGAAATGCCGACACCATACTGGTGGTGGGCGAAGCAGGCAGT
GGCAAGAGCACTCTTCTGCAGCGTTTGCACCTGCTGTGGGCAACAGGGAG
GAGCTTCCAGGAGTTTCTCTTCATTTTCCCATTCAGCTGCCGACAGTTGC
AATGCGTGGCCAAACCGCTGTCCCTGAGGACGCTGCTCTTTGAGCACTGC
TGCTGGCCTGATGTCGCTCAGGACGATGTCTTCCAGTTCCTTCTTGACCA
TCCTGACCGTGTCCTGTTAACCTTTGATGGCTTGGACGAGTTCAAGTTCC
GGTTCACCGACCGGGAGCGCCACTGCTCTCCAATTGACCCCACGTCAGTC
CAGACTCTGCTCTTCAACCTTCTCCAGGGGAACCTGCTGAAGAATGCCTG
CAAGGTGCTGACCAGCCGTCCGGATGCTGTGTCAGCGCTCCTCAGGAAGT
TCGTCCGTACAGAGTGCCAACTGAAGGGCTTCTCTGAAGAGGGCATCCAA
CTGTACCTGAGAAAGCACCACCGGGAACCTGGGGTGGCAGACCGCCTCAT
CCAGCTGATCCAAGCCACCTCAGCCCTGCATGGTTTGTGCCACCTCCCTG
TCTTCTCTTGGATGGTGTCCAGATGCCACCGGGAACTTTTGCTGCAGAAC
AGGGTCTTCCCAACAACCAGCACGGACATGTACCTCCTGATCCTGCAGCA
TTTCCTGCTGCATGCCTCCCCTCCGGACTCCTCCCCACTCGGTCTGGGAC
CTGGACTCCTCCAAAGCCGGCTCTCCACCCTCCTGCACCTTGGCCACCTG
GCTCTCCGGGGCCTGGCCATGAGCTGCTATGTGTTCTCAGCCCAGCAGCT
CCAGGCAGCTCAGGTTGACTCTGATGATATTTCTCTTGGTTTCCTGGTAC
GTGCCCAAAGTAGTGTGCCCGGGAGCAAGGCGCCCCTGGAGTTCCTGCAC
ATTACCTTCCAGTGCTTTTTTGCCGCTTTCTACTTGGCTGTCAGTGCTGA
CACATCAGTGGCCTCTCTCAAGCACCTTTTCAGCTGTGGCCGGCTGGGCA
GCTCACTGCTGGGAAGGCTGCTGCCCAACCTGTGTATCCAGGGCTCCAGA
GTCAAGAAGGGCAGCGAAGCAGCCCTGCTGCAGAAGGCTGAGCCACACAA
CCTGCAGATCACAGCAGCCTTCCTAGCAGGTCTGTTGTCCCAGCAGCATC
GGGACCTGTTGGCTGCATGCCAGATCTCCGAGAGGGTGCTGCTCCAGCGT
CAGGCACGTGCCCGCTCGTGTCTGGCCCACAGCCTCCGCGAGCACTTCCA
TTCCATCCCGCCTGCCGTGCCCGGTGAGACCAAGAGCATGCATGCTATGC
CGGGCTTTATTTGGCTCATCCGGAGCCTGTACGAGATGCAGGAGGAGCAG
TTGGCCCAGGAGGCTGTCCGTCGCTTGGACATCGGGCACCTGAAGTTGAC
ATTTTGCAGAGTGGGCCCTGCAGAGTGTGCTGCGCTGGCCTTTGTACTGC
AACATCTCCAGCGGCCTGTGGCCCTACAGCTGGATTACAACTCTGTGGGA
GATGTCGGAGTGGAACAGCTGCGACCGTGCCTTGGGGTCTGCACAGCTCT
GTATTTGCGAGATAACAATATCTCAGACCGAGGTGCCCGCACGCTGGTTG
AGTGTGCTCTTCGCTGTGAGCAGCTGCAGAAACTAGCTCTCTTCAACAAC
AAACTCACGGATGCGTGCGCCTGCTCCATGGCCAAGCTCCTTGCACACAA
GCAGAACTTCTTGTCCCTGAGGGTGGGGAACAATCACATCACAGCCGCTG
GAGCCGAGGTGCTGGCCCAGGGACTCAAGAGCAACACCTCCCTGAAGTTC
CTGGGGTTCTGGGGCAACAGCGTGGGTGATAAGGGCACCCAAGCCCTGGC
TGAAGTTGTAGCCGACCACCAGAACCTAAAGTGGCTCAGCTTGGTAGGAA
ACAACATTGGCAGCATGGGTGCCCAAGCCCTAGCACTGATGCTGGAGAAG
AACAAGTCACTAGAGGAGCTCTGCCTAGAGGAAAACCATATCTGTGACGA
AGGGGTATATTCTCTCGCAGAAGGACTCAAGAGAAATTCAACTTTGAAAT
TCTTGAAACTGTCCAACAATGGCATCACCTACCGGGGTGCAGAAGCCCTC
TTGCAGGCCCTCAGCAGGAACAGTGCCATTCTGGAGGTTTGGCTTCGAGG
GAACACATTCTCTTTGGAGGAAATCCAAACACTGAGCTCCAGGGACGCCA
GACTCTTGTTGTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMCSQEEFQAQRSQ
LVALLISGSLEGFESILDWLLSWDVLSREDYEGLSLPGQPLSHSARRLLD
TVWNKGVWGCQKLLEAVQEAQANSHTFELYGSWDTHSLHPTRDLQSHRPA
IVRRLYNHVEAMLELAREGGFLSQYECEEIRLPIFTSSQRARRLLDLAAV
KANGLAAFLLQHVRELPAPLPLPYEGYSLCRSRCDRGFTLICLFCILAAE
CQKFISKLRTMVLAQSRFLSTYDGSENLCLEDIYTENILELRTEVGTAGA
LQKSPAILGLEDLFDTHGHLNRNADTILVVGEAGSGKSTLLQRLHLLWAT
GRSFQEFLFIFPFSCRQLQCVAKPLSLRTLLFEHCCWPDVAQDDVFQFLL
DHPDRVLLTFDGLDEFKFRFTDRERHCSPIDPTSVQTLLFNLLQGNLLKN
ACKVLTSRPDAVSALLRKFVRTECQLKGFSEEGIQLYLRKHHREPGVADR
LIQLIQATSALHGLCHLPVFSWMVSRCHRELLLQNRVFPTTSTDMYLLIL
QHFLLHASPPDSSPLGLGPGLLQSRLSTLLHLGHLALRGLAMSCYVFSAQ
QLQAAQVDSDDISLGFLVRAQSSVPGSKAPLEFLHITFQCFFAAFYLAVS
ADTSVASLKHLFSCGRLGSSLLGRLLPNLCIQGSRVKKGSEAALLQKAEP
HNLQITAAFLAGLLSQQHRDLLAACQISERVLLQRQARARSCLAHSLREH
FHSIPPAVPGETKSMHAMPGFIWLIRSLYEMQEEQLAQEAVRRLDIGHLK
LTFCRVGPAECAALAFVLQHLQRPVALQLDYNSVGDVGVEQLRPCLGVCT
ALYLRDNNISDRGARTLVECALRCEQLQKLALFNNKLTDACACSMAKLLA
HKQNFLSLRVGNNHITAAGAEVLAQGLKSNTSLKFLGFWGNSVGDKGTQA
LAEVVADHQNLKWLSLVGNNIGSMGAQALALMLEKNKSLEELCLEENHIC
DEGVYSLAEGLKRNSTLKFLKLSNNGITYRGAEALLQALSRNSAILEVWL
RGNTFSLEEIQTLSSRDARLLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8414
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: As genbank apart from V618A and lacks the first 10 amino acids. n.b. genbank does not start with a methionine. This sequence agrees with the mouse genome.; ; Estimated MW of expressed protein : 142755 Daltons