cDNA Clone Hip-55

• Plasmid: pEGFP-C1-HIP55
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HIP55
• Genbank: NM_014063
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCGGC
GAACCTGAGCCGGAACGGGCCAGCGCTGCAAGAGGCCTACGTGCGGGTGG
TCACCGAGAAGTCCCCGACCGACTGGGCTCTCTTTACCTATGAAGGCAAC
AGCAATGACATCCGCGTGGCTGGCACAGGGGAGGGTGGCCTGGAGGAGAT
GGTGGAGGAGCTCAACAGCGGGAAGGTGATGTACGCCTTCTGCAGAGTGA
AGGACCCCAACTCTGGACTGCCCAAATTTGTCCTCATCAACTGGACAGGC
GAGGGCGTGAACGATGTGCGGAAGGGAGCCTGTGCCAGCCACGTCAGCAC
CATGGCCAGCTTCCTGAAGGGGGCCCATGTGACCATCAACGCACGGGCCG
AGGAGGATGTGGAGCCTGAGTGCATCATGGAGAAGGTGGCCAAGGCTTCA
GGTGCCAACTACAGCTTTCACAAGGAGAGTGGCCGCTTCCAGGACGTGGG
ACCCCAGGCCCCAGTGGGCTCTGTGTACCAGAAGACCAATGCCGTGTCTG
AGATTAAAAGGGTTGGTAAAGACAGCTTCTGGGCCAAAGCAGAGAAGGAG
GAGGAGAACCGTCGGCTGGAGGAAAAGCGGCGGGCCGAGGAGGCACAGCG
GCAGCTGGAGCAGGAGCGCCGGGAGCGTGAGCTGCGTGAGGCTGCACGCC
GGGAGCAGCGCTATCAGGAGCAGGGTGGCGAGGCCAGCCCCCAGAGCAGG
ACGTGGGAGCAGCAGCAAGAAGTGGTTTCAAGGAACCGAAATGAGCAGGA
GTCTGCCGTGCACCCGAGGGAGATTTTCAAGCAGAAGGAGAGGGCCATGT
CCACCACCTCCATCTCCAGTCCTCAGCCTGGCAAGCTGAGGAGCCCCTTC
CTGCAGAAGCAGCTCACCCAACCAGAGACCCACTTTGGCAGAGAGCCAGC
TGCTGCCATCTCAAGGCCCAGGGCAGATCTCCCTGCTGAGGAGCCGGCGC
CCAGCACTCCTCCATGTCTGGTGCAGGCAGAAGAGGAGGCTGTGTATGAG
GAACCTCCAGAGCAGGAGACCTTCTACGAGCAGCCCCCACTGGTGCAGCA
GCAAGGTGCTGGCTCTGAGCACATTGACCACCACATTCAGGGCCAGGGGC
TCAGTGGGCAAGGGCTCTGTGCCCGTGCCCTGTACGACTACCAGGCAGCC
GACGACACAGAGATCTCCTTTGACCCCGAGAACCTCATCACGGGCATCGA
GGTGATCGACGAAGGCTGGTGGCGTGGCTATGGGCCGGATGGCCATTTTG
GCATGTTCCCTGCCAACTACGTGGAGCTCATTGAGTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMYPYDVPDYAMAANLSRNGPALQEAYVRVVTEKSPTD
WALFTYEGNSNDIRVAGTGEGGLEEMVEELNSGKVMYAFCRVKDPNSGLP
KFVLINWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARAEEDVEPEC
IMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKD
SFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQ
GGEASPQSRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSP
QPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLV
QAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCA
RALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYV
ELIE*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8346
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA fusion for mammalian expression.