cDNA Clone NEDD4L

• Synonyms: RSP5; PVNH7; NEDD4-2; NEDD4.2; hNEDD4-2
• Plasmid: pGEX6P2 NEDD4L
• Parent Plasmid: pGEX6P2
• Expressed: GST 3C NEDD4L
• Genbank: NM_001144965
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAG
AAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATA
TGCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGAC
ATGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCA
CCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAG
TGAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACT
CAGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAA
CATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCA
GGCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTC
CCCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTC
TCTCGGTCTGGCTCTGCCCCCACCGCCGGCCTCCCCAGGATCTCGGACCA
GCCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCA
GACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTC
AAGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATC
TACCACCGCCCAGTGCCCCAGCTGGGAGAGCGCGTTCATCAACTGTCACG
GGTGGTGAGGAACCAACGCCATCAGTGGCCTATGTACATACCACGCCGGG
TCTGCCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACT
ATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAG
CTTGCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCT
AATCGAGCCTCAGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAA
CTTTATCTGCCCCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCT
GTGAAAGACACCCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAA
CTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCT
GGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAAC
ACAAAGACTACAACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATAT
GCGGTCAAAGACATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTG
GCTGGGAAGAAAGAATTCACTTGGATGGCCGAACGTTTTATATTGATCAT
AATAGCAAAATTACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTAT
TACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACT
ACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCTAATAGGTTTGAA
ATGAAACTTCACAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTAT
GTCCGTGAAAAGACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTG
AATCAGAGAAAGGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTC
TTACTGTCCAAAGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTC
TGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTA
ATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTG
GCCGTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTA
CAAGATGATGTTGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGG
ATAGTGAATATTACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACT
GAGCTGGACCTCATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATA
TCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAA
ACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGG
GTCCAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCC
TATTGATTTGATTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGT
GCGGCCTCGGTGATGTGGATGTGAATGACTGGAGACAGCATTCTATTTAC
AAGAACGGCTACTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGC
TGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCA
CAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCC
AATGGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACT
GCCCAGAGCTCACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAA
CCTTTGAAGATTTACGAGAGAAACTTCTCATGGCCGTGGAAAATGCTCAA
GGATTTGAAGGGGTGGATTAAGCGGCCGC

Amino Acid Sequence

Updated data following sequencing. MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAASVMDICRIRPSAAERPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEENSDQR
DDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGG
DVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSST
VTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVR
RAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYI
DHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREW
FFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILEND
PTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHS
IYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVEN
AQGFEGVD*


Original data in database MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDERPYTFKDFLLRP
RSHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQ
HQEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHRPSLMDVSSESDN
NIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGD
SLGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNGEQFSSLIQREPS
SRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTP
GLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNH
LIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPY
NSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVH
MRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPA
ITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRDNIFEESYRRI
MSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEY
SATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPF
YKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQT
YQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELL
PIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWK
AVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEK
LPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P2 NEDD4L442.97 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU8073
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Do not use. 30 extra amino acids between cleavage and protein. See SnapGene file.; Sequencing carried out in Sept 2022.; Bacterial expression plasmid with N terminal GST tag. ; Estimated MW of expressed protein : 125453 Daltons
• Low Temp: No