cDNA Clone CRIF

• Plasmid: pEBG6P-CRIF
• Parent Plasmid: pEBG6P
• Expressed: GST-CRIF
• Genbank: AAL85877
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGTCCGTGCGACAGGCACGCAGCCTACTAGGTGTGGC
GGCGACCCTGGCCCCGGGTTCCCGTGGCTACCGGGCGCGGCCGCCCCCGC
GCCGCAGGCCGGGACCCCGGTGGCCAGACCCCGAGGACCTCCTGACCCCG
CGGTGGCAGCTGGGACCGCGCTACGCGGCTAAGCAGTTCGCGCGTTACGG
CGCCGCCTCCGGGGTGGTCCCCGGTTCGTTATGGCCGTCGCCGGAGCAGC
TGCGGGAGCTGGAGGCCGAAGAACGCGAATGGTACCCGAGCCTGGCGACC
ATGCAGGAGTCGCTGCGGGTGAAGCAGCTGGCCGAAGAGCAGAAGCGTCG
GGAGAGGGAGCAGCACATCGCAGAGTGCATGGCCAAGATGCCACAGATGA
TTGTGAACTGGCAGCAGCAGCAGCGGGAGAACTGGGAGAAGGCCCAGGCT
GACAAGGAGAGGAGGGCCCGACTGCAGGCTGAGGCCCAGGAGCTCCTGGG
CTACCAGGTGGACCCAAGGAGTGCCCGCTTCCAGGAGCTGCTCCAGGACC
TAGAGAAGAAGGAGCGCAAGCGCCTCAAGGAGGAAAAACAGAAACGGAAG
AAGGAGGCGCGAGCTGCTGCATTGGCTGCAGCTGTGGCTCAAGACCCAGC
AGCCTCTGGGGCACCCAGCTCCTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAASVRQARSLLG
VAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFAR
YGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQK
RREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQEL
LGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQD
PAASGAPSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8063
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 52768 Daltons