cDNA Clone Filamin A alpha

• Plasmid: pEBG6P-Filamin A alpha
• Parent Plasmid: pEBG6P
• Expressed: GST-Filamin A alpha
• Genbank: BC014654
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCCTCAGGCAAGGTGGCGCAGCCCACCATCACTGACAACAA
AGACGGCACCGTGACCGTGCGGTATGCACCCAGCGAGGCTGGCCTGCACG
AGATGGACATCCGCTATGACAACATGCACATCCCAGGAAGCCCCTTGCAG
TTCTATGTGGATTACGTCAACTGTGGCCATGTCACTGCCTATGGGCCTGG
CCTCACCCATGGAGTAGTGAACAAGCCTGCCACCTTCACCGTCAACACCA
AGGATGCAGGAGAGGGGGGCCTGTCTCTGGCCATTGAGGGCCCGTCCAAA
GCAGAAATCAGCTGCACTGACAACCAGGATGGGACATGCAGCGTGTCCTA
CCTGCCTGTGCTGCCGGGGGACTACAGCATTCTAGTCAAGTACAATGAAC
AGCACGTCCCAGGCAGCCCCTTCACTGCTCGGGTCACAGGTGACGACTCC
ATGCGTATGTCCCACCTAAAGGTCGGCTCTGCTGCCGACATCCCCATCAA
CATCTCAGAGACGGATCTCAGCCTGCTGACGGCCACTGTGGTCCCGCCCT
CGGGCCGGGAGGAGCCCTGTTTGCTGAAGCGGCTGCGTAATGGCCACGTG
GGGATTTCATTCGTGCCCAAGGAGACGGGGGAGCACCTGGTGCATGTGAA
GAAAAATGGCCAGCACGTGGCCAGCAGCCCCATCCCGGTGGTGATCAGCC
AGTCGGAAATTGGGGATGCCAGTCGTGTTCGGGTCTCTGGTCAGGGCCTT
CACGAAGGCCACACCTTTGAGCCTGCAGAGTTTATCATTGATACCCGCGA
TGCAGGCTATGGTGGGCTCAGCCTGTCCATTGAGGGCCCCAGCAAGGTGG
ACATCAACACAGAGGACCTGGAGGACGGGACGTGCAGGGTCACCTACTGC
CCCACAGAGCCAGGCAACTACATCATCAACATCAAGTTTGCCGACCAGCA
CGTGCCTGGCAGCCCCTTCTCTGTGAAGGTGACAGGCGAGGGCCGGGTGA
AAGAGAGCATCACCCGCAGGCGTCGGGCTCCTTCAGTGGCCAACGTTGGT
AGTCATTGTGACCTCAGCCTGAAAATCCCTGAAATTAGCATCCAGGATAT
GACAGCCCAGGTGACCAGCCCATCGGGCAAGACCCATGAGGCCGAGATCG
TGGAAGGGGAGAACCACACCTACTGCATCCGCTTTGTTCCCGCTGAGATG
GGCACACACACAGTCAGCGTGAAGTACAAGGGCCAGCACGTGCCTGGGAG
CCCCTTCCAGTTCACCGTGGGGCCCCTAGGGGAAGGGGGAGCCCACAAGG
TCCGAGCTGGGGGCCCTGGCCTGGAGAGAGCTGAAGCTGGAGTGCCAGCC
GAATTCAGTATCTGGACCCGGGAAGCTGGTGCTGGAGGCCTGGCCATTGC
TGTCGAGGGCCCCAGCAAGGCTGAGATCTCTTTTGAGGACCGCAAGGACG
GCTCCTGTGGTGTGGCTTATGTGGTCCAGGAGCCAGGTGACTACGAAGTC
TCAGTCAAGTTCAACGAGGAACACATTCCCGACAGCCCCTTCGTGGTGCC
TGTGGCTTCTCCGTCTGGCGACGCCCGCCGCCTCACTGTTTCTAGCCTTC
AGGAGTCAGGGCTAAAGGTCAACCAGCCAGCCTCTTTTGCAGTCAGCCTG
AACGGGGCCAAGGGGGCGATCGATGCCAAGGTGCACAGCCCCTCAGGAGC
CCTGGAGGAGTGCTATGTCACAGAAATTGACCAAGATAAGTATGCTGTGC
GCTTCATCCCTCGGGAGAATGGCGTTTACCTGATTGACGTCAAGTTCAAC
GGCACCCACATCCCTGGAAGCCCCTTCAAGATCCGAGTTGGGGAGCCTGG
GCATGGAGGGGACCCAGGCTTGGTGTCTGCTTACGGAGCAGGTCTGGAAG
GCGGTGTCACAGGGAACCCAGCTGAGTTCGTCGTGAACACGAGCAATGCG
GGAGCTGGTGCCCTGTCGGTGACCATTGACGGCCCCTCCAAGGTGAAGAT
GGATTGCCAGGAGTGCCCTGAGGGCTACCGCGTCACCTATACCCCCATGG
CACCTGGCAGCTACCTCATCTCCATCAAGTACGGCGGCCCCTACCACATT
GGGGGCAGCCCCTTCAAGGCCAAAGTCACAGGCCCCCGTCTCGTCAGCAA
CCACAGCCTCCACGAGACATCATCAGTGTTTGTAGACTCTCTGACCAAGG
CCACCTGTGCCCCCCAGCATGGGGCCCCGGGTCCTGGGCCTGCTGACGCC
AGCAAGGTGGTGGCCAAGGGCCTGGGGCTGAGCAAGGCCTACGTAGGCCA
GAAGAGCAGCTTCACAGTAGACTGCAGCAAAGCAGGCAACAACATGCTGC
TGGTGGGGGTTCATGGCCCAAGGACCCCCTGCGAGGAGATCCTGGTGAAG
CACGTGGGCAGCCGGCTCTACAGCGTGTCCTACCTGCTCAAGGACAAGGG
GGAGTACACACTGGTGGTCAAATGGGGGGACGAGCACATCCCAGGCAGCC
CCTACCGCGTTGTGGTGCCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPSGKVAQPTITD
NKDGTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHVTAYG
PGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGTCSV
SYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIP
INISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVH
VKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDT
RDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFAD
QHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQ
DMTAQVTSPSGKTHEAEIVEGENHTYCIRFVPAEMGTHTVSVKYKGQHVP
GSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLA
IAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFV
VPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPS
GALEECYVTEIDQDKYAVRFIPRENGVYLIDVKFNGTHIPGSPFKIRVGE
PGHGGDPGLVSAYGAGLEGGVTGNPAEFVVNTSNAGAGALSVTIDGPSKV
KMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLV
SNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYV
GQKSSFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHVGSRLYSVSYLLKD
KGEYTLVVKWGDEHIPGSPYRVVVP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8043
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 115934 Daltons