cDNA Clone FLJ21128

• Plasmid: pCMV-HA-1 FLJ21128
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-FLJ21128
• Genbank: NP_079359
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGG
AGATAGCTTGGGTGTCTATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCA
GCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTGTCTT
GTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCT
GGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCAAA
CAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAAT
GGCACAGGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCA
GAATATCCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCC
CGCAGCAGCAACAGTGCTCAAAGAGCTATGTCGACAGGCACATGGAATCC
TTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCATCTAG
TAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATG
GCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAG
ATCCCAGACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAA
GGCAGCTGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCC
GTTCCCGGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAG
AACATCTTGGAGTCCGAGCCCATTGTCTATCGACGGATCATAGTGCCCCA
CAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCAAGTA
TTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGG
CTGACCCTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGAT
GGCACTGACCCTCCTCGGAGGACCTAACAGGTTGAATCCCAAAAATGTTC
ACCAGAGGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCT
CAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCAT
CCTTTTCCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAATGAGC
TGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGAT
CTGCCCACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGA
GAACGTCTTCCTGCGCGATCAACCCTGGTACAAGGCAGCTGTGGCCTGGG
CCAACCAGAACCGGGCACCAGTACTCAGCATAGACCCTCCTGTGCATGAA
GTCGAACAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCT
GCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTC
CCCAGCAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGC
TGCAAGTTTGTTATCCCACTGCACTCTGCTTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLT
RPFHNGVKCLVPEVTFRAGDITELKILEIPGPGDNQHFGDLHQTELGPSG
AGCQVGINQNGTGKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCS
KSYVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKD
DECFGDDIEEIPDTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPN
ERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHK
KLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVA
LLCGPHVKGAQGISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKT
QGQQVSSLKDLPTSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAP
VLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQE
VGINYHSPFGCKFVIPLHSA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8031
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression.; Estimated MW of expressed protein : 57400 Daltons