cDNA Clone MLK4

• Plasmid: pFBDual6P-1 MLK4
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST MLK4
• Genbank: BC136649.1
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggctttgcggggcgccgcgggagcgaccgacaccccggtgtc
ctcggccgggggagcccccggcggctcagcgtcctcgtcgtccacctcct
cgggcggctcggcctcggcgggcgcggggctgtgggccgcgctctatgac
tacgaggctcgcggcgaggacgagctgagcctgcggcgcggccagctggt
ggaggtgctgtcgcaggacgccgccgtgtcgggcgacgagggctggtggg
caggccaggtgcagcggcgcctcggcatcttccccgccaactacgtggct
ccctgccgcccggccgccagccccgcgccgccgccctcgcggcccagctc
cccggtacacgtcgccttcgagcggctggagctgaaggagctcatcggcg
ctgggggcttcgggcaggtgtaccgcgccacctggcagggccaggaggtg
gccgtgaaggcggcgcgccaggacccggagcaggacgcggcggcggctgc
cgagagcgtgcggcgcgaggctcggctcttcgccatgctgcggcacccca
acatcatcgagctgcgcggcgtgtgcctgcagcagccgcacctctgcctg
gtgctggagttcgcccgcggcggagcgctcaaccgagcgctggccgctgc
caacgccgccccggacccgcgcgcgcccggcccccgccgcgcgcgccgca
tccctccgcacgtgctggtcaactgggccgtgcagatagcgcggggcatg
ctctacctgcatgaggaggccttcgtgcccatcctgcaccgggacctcaa
gtccagcaacattttgctacttgagaagatagaacatgatgacatctgca
ataaaactttgaagattacagattttgggttggcgagggaatggcacagg
accaccaaaatgagcacagcaggcacctatgcctggatggcccccgaagt
gatcaagtcttccttgttttctaagggaagcgacatctggagctatggag
tgctgctgtgggaactgctcaccggagaagtcccctatcggggcattgat
ggcctcgccgtggcttatggggtagcagtcaataaactcactttgcccat
tccatccacctgccctgagccgtttgccaagctcatgaaagaatgctggc
aacaagaccctcatattcgtccatcgtttgccttaattctcgaacagttg
actgctattgaaggggcagtgatgactgagatgcctcaagaatcttttca
ttccatgcaagatgactggaaactagaaattcaacaaatgtttgatgagt
tgagaacaaaggaaaaggagctgcgatcccgggaagaggagctgactcgg
gcggctctgcagcagaagtctcaggaggagctgctaaagcggcgtgagca
gcagctggcagagcgcgagatcgacgtgctggagcgggaacttaacattc
tgatattccagctaaaccaggagaagcccaaggtaaagaagaggaagggc
aagtttaagagaagtcgtttaaagctcaaagatggacatcgaattagttt
accttcagatttccagcacaagataaccgtgcaggcctctcccaacttgg
acaaacggcggagcctgaacagcagcagttccagtcccccgagcagcccc
acaatgatgccccgactccgagccatacagttgacttcagatgaaagcaa
taaaacttggggaaggaacacagtctttcgacaagaagaatttgaggatg
taaaaaggaattttaagaaaaaaggttgtacctggggaccaaattccatt
caaatgaaagatagaacagattgcaaagaaaggataagacctctctccga
tggcaacagtccttggtcaactatcttaataaaaaatcagaaaaccatgc
ccttggcttcattgtttgtggaccagccagggtcctgtgaagagccaaaa
ctttcccctgatggattagaacacagaaaaccaaaacaaataaaattgcc
tagtcaggcctacattgatctacctcttgggaaagatgctcagagagaga
atcctgcagaagctgaaagctgggaggaggcagcctctgcgaatgctgcc
acagtctccattgagatgactcctacgaatagtctgagtagatcccccca
gagaaagaaaacggagtcagctctgtatgggtgcaccgtccttctggcat
cggtggctctgggactggacctcagagagcttcataaagcacaggctgct
gaagaaccgttgcccaaggaagagaagaagaaacgagagggaatcttcca
gcgggcttccaagtcccgcagaagcgccagtcctcccacaagcctgccat
ccacctgtggggaggccagcagcccaccctccctgccactgtcaagtgcc
ctgggcatcctctccacaccttctttctccacaaagtgcctgctgcagat
ggacagtgaagatccactggtggacagtgcacctgtcacttgtgactctg
agatgctcactccggatttttgtcccactgccccaggaagtggtcgtgag
ccagccctcatgccaagacttgacactgattgtagtgtatcaagaaactt
gccgtcttccttcctacagcagacatgtgggaatgtaccttactgtgctt
cttcaaaacatagaccgtcacatcacagacggaccatgtctgatggaaat
ccgaccccaactggtgcaactattatctcagccactggagcctctgcact
gccactctgcccctcacctgctcctcacagtcatctgccaagggaggtct
cacccaagaagcacagcactgtccacatcgtgcctcagcgtcgccctgcc
tccctgagaagccgctcagatctgcctcaggcttacccacagacagcagt
gtctcagctggcacagactgcctgtgtagtgggtcgcccaggaccacatc
ccacccaattcctcgctgccaaggagagaactaaatcccatgtgccttca
ttactggatgctgacgtggaaggtcagagcagggactacactgtgccact
gtgcagaatgaggagcaaaaccagccggccatctatatatgaactggaga
aagaattcctgtcttaagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMALRGAAGATDTPVSSAGG
APGGSASSSSTSSGGSASAGAGLWAALYDYEARGEDELSLRRGQLVEVLS
QDAAVSGDEGWWAGQVQRRLGIFPANYVAPCRPAASPAPPPSRPSSPVHV
AFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVR
REARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAP
DPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNI
LLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSS
LFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTC
PEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQESFHSMQD
DWKLEIQQMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAE
REIDVLERELNILIFQLNQEKPKVKKRKGKFKRSRLKLKDGHRISLPSDF
QHKITVQASPNLDKRRSLNSSSSSPPSSPTMMPRLRAIQLTSDESNKTWG
RNTVFRQEEFEDVKRNFKKKGCTWGPNSIQMKDRTDCKERIRPLSDGNSP
WSTILIKNQKTMPLASLFVDQPGSCEEPKLSPDGLEHRKPKQIKLPSQAY
IDLPLGKDAQRENPAEAESWEEAASANAATVSIEMTPTNSLSRSPQRKKT
ESALYGCTVLLASVALGLDLRELHKAQAAEEPLPKEEKKKREGIFQRASK
SRRSASPPTSLPSTCGEASSPPSLPLSSALGILSTPSFSTKCLLQMDSED
PLVDSAPVTCDSEMLTPDFCPTAPGSGREPALMPRLDTDCSVSRNLPSSF
LQQTCGNVPYCASSKHRPSHHRRTMSDGNPTPTGATIISATGASALPLCP
SPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLA
QTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMR
SKTSRPSIYELEKEFLS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU35607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee