cDNA Clone LST8; TOR

• Plasmid: pGEX4P-LST8, mTOR 2150-2384
• Parent Plasmid: pGEX4T1
• Expressed: GST-C3 LST8, mTOR 2150-2384
• Genbank: NP_071767
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCTGGAAGTTCTGTTCCAGGGCCCGATGAACACCTCCCCAGGCACGGTGGGCAGTGACCCGGTCATCCTGGCCACTGCAGGCTACGACCACACCGTGCGCTTCTGGCAGGCCCACAGCGGCATCTGCACCCGGACGGTGCAGCACCAGGACTCCCAGGTGAATGCCTTGGAGGTCACACCGGACCGCAGCATGATTGCTGCTGCAGGTTACCAGCACATCCGCATGTATGATCTCAACTCCAATAACCCTAACCCCATCATCAGCTACGACGGCGTCAACAAGAACATCGCGTCTGTGGGCTTCCACGAAGACGGCCGCTGGATGTACACGGGCGGCGAGGACTGCACAGCCAGGATCTGGGACCTCAGGTCCCGGAACCTGCAGTGCCAGCGGATCTTCCAGGTGAACGCACCCATTAACTGCGTGTGCCTGCACCCCAACCAGGCAGAGCTCATCGTGGGTGACCAGAGCGGGGCTATCCACATCTGGGACTTGAAAACAGACCACAACGAGCAGCTGATCCCTGAGCCCGAGGTCTCCATCACGTCCGCCCACATCGATCCCGACGCCAGCTACATGGCAGCTGTCAATAGCACCGGAAACTGCTATGTCTGGAATCTGACGGGGGGCATTGGTGACGAGGTGACCCAGCTCATCCCCAAGACTAAGATCCCTGCCCACACGCGCTACGCCCTGCAGTGTCGCTTCAGCCCCGACTCCACGCTCCTCGCCACCTGCTCGGCTGATCAGACGTGCAAGATCTGGAGGACGTCCAACTTCTCCCTGATGACGGAGCTGAGCATCAAGAGCGGCAACCCCGGGGAGTCCTCCCGCGGCTGGATGTGGGGCTGCGCCTTCTCGGGGGACTCCCAGTACATCGTCACTGCTTCCTCGGACAACCTGGCCCGGCTCTGGTGTGTGGAGACTGGAGAGATCAAGAGAGAGTATGGCGGCCACCAGAAGGCTGTTGTCTGCCTGGCCTTCAATGACAGTGTGCTGGGCTAGCGGCCGC

DNA SEQ OF TOR WITH USPTREAM BACTERIAL ENAHNCER SEQ.
GCGGCCGCAATAATTTTGTTTAACTTTAAGAAGGAGATATAAATATGATCATTCGCATTCAGTCCATAGCACCGTCTTTGCAAGTCATCACATCCAAGCAGAGGCCCCGGAAATTGACACTTATGGGCAGCAACGGACATGAGTTTGTTTTCCTTCTAAAAGGCCATGAAGATCTGCGCCAGGATGAGCGTGTGATGCAGCTCTTCGGCCTGGTTAACACCCTTCTGGCCAATGACCCAACATCTCTTCGGAAAAACCTCAGCATCCAGAGATACGCTGTCATCCCTTTATCGACCAACTCGGGCCTCATTGGCTGGGTTCCCCACTGTGACACACTGCACGCCCTCATCCGGGACTACAGGGAGAAGAAGAAGATCCTTCTCAACATCGAGCATCGCATCATGTTGCGGATGGCTCCGGACTATGACCACTTGACTCTGATGCAGAAGGTGGAGGTGTTTGAGCATGCCGTCAATAATACAGCTGGGGACGACCTGGCCAAGCTGCTGTGGCTGAAAAGCCCCAGCTCCGAGGTGTGGTTTGACCGAAGAACCAATTATACCCGTTCTTTAGCGGTCATGTCAATGGTTGGGTATATTTTAGGCCTGGGAGATAGACACCCATCCAACCTGATGCTGGACCGTCTGAGTGGGAAGATCCTGCACATTGACTTTGGGGACTGCTTTGAGGTTGCTATGACCCGAGAGAAGTTTCCAGAGAAGATTCCATTTAGACTAACAAGAATGTTGACCAATGCTATGGAGGTTACAGGCTAGCGGCCGC

Amino Acid Sequence

GST-LST8:
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPMNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG*
MTOR:
MIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTG*

• Vector Type: Bacterial
• DU Number: DU13893
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee