cDNA Clone
SGK-495

Plasmid
GFP-TAP SGK495+STREP
Parent Plasmid
pEGFP TAP
Expressed
GFP-TAP-SGK495+STREP
Genbank
BC007835, AY198395
Tag
GFP
TAP
Species
Human
Sequence of Insert
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTCGATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACGCGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGATCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTCCAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGAAGCGGAGAGCATCAGACAGAGGAGCTGGGGAAACGTCGGCCAGGGCCAAGGCTCTAGGAAGTGGGATTTCTGGAAATAATGCAAAGAGAGCTGGACCATTCATCCTTGGTCCCCGTCTGGGCAACTCACCGGTGCCAAGCATAGTGCAGTGTTTGGCAAGGAAAGATGGCACGGATGACTTCTATCAGCTGAAGATCCTGACCCTGGAGGAGAGGGGGGACCAAGGCATAGAGAGCCAGGAAGAGCGGCAGGGCAAGATGCTGCTGCACACCGAGTACTCACTGCTGTCTCTCCTGCACACGCAGGATGGCGTGGTGCACCACCACGGCCTCTTCCAGGACCGCACCTGTGAAATCGTTGAGGACACAGAATCCAGCCGGATGGTTAAGAAGATGAAGAAGCGCATCTGCCTCGTCCTGGACTGCCTCTGTGCTCATGACTTCAGCGATAAGACCGCTGACCTCATCAACCTGCAGCACTACGTCATCAAGGAGAAGAGGCTCAGCGAGAGGGAGACTGTGGTAATCTTCTACGACGTGGTCCGCGTGGTGGAGGCCCTGCACCAGAAAAATATCGTGCACAGAGACCTGAAGCTGGGGAACATGGTGCTCAACAAGAGGACACATCGGATAACCATCACCAACTTCTGCCTCGGGAAGCATCTGGTGAGCGAGGGGGACCTGCTGAAGGACCAGAGAGGGAGCCCTGCCTACATCAGTCCCGACGTGCTCAGCGGCCGGCCGTACCGTGGCAAGCCCAGTGACATGTGGGCCCTGGGCGTGGTGCTCTTCACCATGCTGTATGGCCAGTTCCCCTTCTACGACAGCATCCCGCAGGAGCTCTTCCGCAAGATCAAGGCTGCCGAGTATACCATTCCTGAGGATGGACGGGTTTCTGAGAACACCGTGTGTCTCATCCGGAAGCTGCTGGTCCTTGACCCCCAGCAGCGCCTGGCCGCCGCCGACGTCCTGGAGGCCCTCAGTGCCATCATTGCATCATGGCAGTCCCTGTCATCTCTGAGTGGGCCTTTGCAAGTGGTTCCTGACATTGATGACCAAATGAGCAATGCGGATAGCTCCCAGGAGGCGAAGGTGACGGAGGAGTGCTCCCAGTACGAGTTTGAGAACTACATGCGTCAGCAGCTGCTGCTGGCCGAGGAGAAGAGCTCCATCCATGACGCCCGGAGCTGGGTACCCAAGCGGCAGTTCGGCAGCGCACCACCGGTGCGACGGCTGGGCCACGACGCACAGCCCATGACCTCCTTGGACACGGCCATCCTGGCGCAGCGCTACCTGCGGAAATGGAGTCATCCACAGTTCGAGAAAGGAGGTGGCAGTGGTGGCGGATCTGGAGGTGGCTCATGGAGTCATCCACAGTTCGAGAAATGAGCGGCCGC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFDIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSATMYPYDVPDYAMKRRASDRGAGETSARAKALGSGISGNNAKRAGPFILGPRLGNSPVPSIVQCLARKDGTDDFYQLKILTLEERGDQGIESQEERQGKMLLHTEYSLLSLLHTQDGVVHHHGLFQDRTCEIVEDTESSRMVKKMKKRICLVLDCLCAHDFSDKTADLINLQHYVIKEKRLSERETVVIFYDVVRVVEALHQKNIVHRDLKLGNMVLNKRTHRITITNFCLGKHLVSEGDLLKDQRGSPAYISPDVLSGRPYRGKPSDMWALGVVLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPEDGRVSENTVCLIRKLLVLDPQQRLAAADVLEALSAIIASWQSLSSLSGPLQVVPDIDDQMSNADSSQEAKVTEECSQYEFENYMRQQLLLAEEKSSIHDARSWVPKRQFGSAPPVRRLGHDAQPMTSLDTAILAQRYLRKWSHPQFEKGGGSGGGSGGGSWSHPQFEK*AAARAN
Vector Type
Mammalian
Antibiotic
Kan
DU Number
DU2731
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
Amplified from EST NCBI.Acc. BQ643074, IMAGE 6305148
Using primers :
1.GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGAAGCGGAGAGCATCAGACAGAGGA

2.CCAGATCCGCCACCACTGCCACCTCCTTTCTCGAACTGTGGATGACTCCATTTCCGCAGGTAGCGCTGCGCCAGGCATGAAGCGGAGAGCATCAGACAGAGG

3.GCGGCCGCTCATTTCTCGAACTGTGGATGACTCCATGAGCCACCTCCAGATCCGCCACCACTGCCACCTC
Price
£125.00
To submit an order for this reagent acceptance of the Dundee Material Transfer Agreement is required at checkout.