cDNA Clone MARK4

• Synonyms: MARK4L, MARK4S, MARKL1, MARKL1L, PAR-1D
• Plasmid: pCMV5 HA MARK4 D199A (Kinase Dead)
• Parent Plasmid: pCMV5
• Expressed: HA-MARK4 D199A (Kinase Dead)
• Genbank: NM_001199867.1
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCTTCGCGGACGGTGCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATGGCACCTTGGGCAGTGGCCGCTCCTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGCCCGTTGCCGGAACTCCATCGCCTCCTGTCCCGAGGAGCAGCCCCACGTGGGCAACTACCGCCTGCTGAGGACCATTGGGAAGGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACATCCTCACTGGTCGGGAGGTTGCCATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCAGCCTGCAGAAGCTGTTTCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAACATCGTGAAGCTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGGTGATGGAGTACGCAAGTGCTGGAGAAGTGTTTGACTACCTCGTGTCGCATGGCCGCATGAAGGAGAAGGAAGCTCGAGCCAAGTTCCGACAGATTGTTTCGGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAGGGACCTGAAGGCTGAGAACCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGCCTTTGGCTTCAGCAACGAGTTCACGCTGGGATCGAAGCTGGACACGTTCTGCGGGAGCCCCCCATATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACGGGCCGGAGGTGGACATCTGGAGCCTGGGAGTCATCCTGTACACCCTCGTCAGCGGCTCCCTGCCCTTCGACGGGCACAACCTCAAGGAGCTGCGGGAGCGAGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACATGTCAACAGACTGTGAGAGCATCCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCTGTACTCTCGAGCAAATCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGTTGAAGCCATACACAGAGCCCGAGGAGGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAGTCCTTGACCAGCCAGAAGTACAACGAAGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGGGTGGGGACCGGGGCGCCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGAACAAGTTCCAGCAAAGGCACCAGCCACAGCAAAGGGCAGCGGAGTTCCTCTTCCACCTACCACCGCCAGCGCAGGCATAGCGATTTCTGTGGCCCATCCCCTGCACCCCTGCACCCCAAACGCAGCCCGACGAGCACGGGGGAGGCGGAGCTGAAGGAGGAGCGGCTGCCAGGCCGGAAGGCGAGCTGCAGCACCGCGGGGAGTGGGAGTCGAGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCGCCCACAACCCCAACAAGGCAGAGATCCCAGAGCGGCGGAAGGACAGCACGAGCACCCCCAACAACCTCCCTCCTAGCATGATGACCCGCAGAAACACCTACGTTTGCACAGAACGCCCGGGGGCTGAGCGCCCGTCACTGTTGCCAAATGGGAAAGAAAACAGCTCAGGCACCCCACGGGTGCCCCCTGCCTCCCCCTCCAGTCACAGCCTGGCACCCCCATCAGGGGAGCGGAGCCGCCTGGCACGCGGTTCCACCATCCGCAGCACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGGCAGGGGGTGGGGGTGGTGGGGGTGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCCATGAGGCTGCACCCCTGCCCGCCGGGCGGCCCCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAGGGTCGCAGACGAACCTGAGAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATCAAACGGAAACCGCCCCCCGGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTCGCGCCCTCCTGAGGCCCTGATGGCAGCTCTGCGCCAGGCCACAGCAGCCGCCCGCTGCCGCTGCCGCCAGCCACAGCCGTTCCTGCTGGCCTGCCTGCACGGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCACTTCGAAGTGGAGGTCTGCCAGCTGCCCCGGCCAGGCTTGCGGGGAGTTCTCTTCCGCCGTGTGGCGGGCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTCCAACGACCTCGAGCTCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYASSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGAR
CRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIID
KTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAG
EVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDA
EANIKIAAFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWS
LGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFL
VLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGM
GYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPS
DTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTS
TGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERR
KDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPP
ASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGP
PASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTS
CHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQP
QPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRT
LVTRISNDLEL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU2324
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kinase Dead (D199A). Contains a silent C>T change at 312 bp as compared to the reference sequence.