cDNA Clone BRSK2

• Plasmid: GFP-TAP BRSK2-Kdead D1598/A
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-BRSK2 Kdead D159/A
• Genbank: AF533878, NO 1ST Met, S251 is I in our protein
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGGCTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGTGGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGGCCAGCTGTGGGTCCCCCCACTACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCCATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAACTCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCGACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCCTGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSFDIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGRSMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSATMYPYDVPDYATSTGKDGGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLEASCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU2269
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid.