cDNA Clone BRSK2

• Plasmid: pCMV BRSK2-Kdead D158/A
• Parent Plasmid: pCMV5
• Expressed: HA-BRSK2-Kdead D159/A
• Genbank: AF533878
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCCTTGGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGGCTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGTGGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGCCTTTGGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCACTACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCCATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAACTCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCGACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCCTGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGAGCGGCCGCAAGGGCGAATTC

Amino Acid Sequence

MYPYDVPDYATSTGKDGGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIAAFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU2229
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee