cDNA Clone MYH9

• Plasmid: pCMV-FLAG3 MYH9
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG3 MYH9
• Genbank: NM_002473
• Tag: FLAG
• Species: Human

Sequence of Insert

gtcgacatggcacagcaagctgccgataagtatctctatgtggataaaaacttcatcaacaatccgctggcccaggccgactgggctgccaagaagctggtatgggtgccttccgacaagagtggctttgagccagccagcctcaaggaggaggtgggcgaagaggccatcgtggagctggtggagaatgggaagaaggtgaaggtgaacaaggatgacatccagaagatgaacccgcccaagttctccaaggtggaggacatggcagagctcacgtgcctcaacgaagcctcggtgctgcacaac
ctcaaggagcgttactactcagggctcatctacacctattcaggcctgtt
ctgtgtggtcatcaatccttacaagaacctgcccatctactctgaagaga
ttgtggaaatgtacaagggcaagaagaggcacgagatgccccctcacatc
tatgccatcacagacaccgcctacaggagtatgatgcaagaccgagaaga
tcaatccatcttgtgcactggtgaatctggagctggcaagacggagaaca
ccaagaaggtcatccagtatctggcgtacgtggcgtcctcgcacaagagc
aagaaggaccagggcgagctggagcggcagctgctgcaggccaaccccat
cctggaggccttcgggaacgccaagaccgtgaagaatgacaactcctccc
gcttcggcaaattcattcgcatcaactttgatgtcaatggctacattgtt
ggagccaacattgagacttatcttttggagaaatctcgtgctatccgcca
agccaaggaagaacggaccttccacatcttctattatctcctgtctgggg
ctggagagcacctgaagaccgatctcctgttggagccgtacaacaaatac
cgcttcctgtccaatggacacgtcaccatccccgggcagcaggacaagga
catgttccaggagaccatggaggccatgaggattatgggcatcccagaag
aggagcaaatgggcctgctgcgggtcatctcaggggttcttcagctcggc
aacatcgtcttcaagaaggagcggaacactgaccaggcgtccatgcccga
caacacagctgcccaaaaggtgtcccatctcttgggtatcaatgtgaccg
atttcaccagaggaatcctcaccccgcgcatcaaggtgggacgggattac
gtccagaaggcgcagactaaagagcaggctgactttgccatcgaggcctt
ggccaaggcgacctatgagcggatgttccgctggctggtgctgcgcatca
acaaggctctggacaagaccaagaggcagggcgcctccttcatcgggatc
ctggacattgccggcttcgagatctttgatctgaactcgtttgagcagct
gtgcatcaattacaccaatgagaagctgcagcagctcttcaaccacacca
tgttcatcctggagcaggaggagtaccagcgcgagggcatcgagtggaac
ttcatcgactttggcctcgacctgcagccctgcatcgacctcattgagaa
gccagcaggccccccgggcattctggccctgctggacgaggagtgctggt
tccccaaagccaccgacaagagcttcgtggagaaggtgatgcaggagcag
ggcacccaccccaagttccagaagcccaagcagctgaaggacaaagctga
tttctgcattatccactatgccggcaaggtggattacaaagctgacgagt
ggctgatgaagaacatggatcccctgaatgacaacatcgccacactgctc
caccagtcctctgacaagtttgtctcggagctgtggaaggatgtggaccg
catcatcggcctggaccaggtggccggcatgtcggagaccgcactgcccg
gggccttcaagacgcggaagggcatgttccgcactgtggggcagctttac
aaggagcagctggccaagctgatggctacgctgaggaacacgaaccccaa
ctttgtccgctgcatcatccccaaccacgagaagaaggccggcaagctgg
acccgcatctcgtgctggaccagctgcgctgcaacggtgttctcgagggc
atccgtatctgccgccagggcttccccaacagggtggtcttccaggagtt
tcggcagagatatgagatcctgactccaaactccattcccaagggtttca
tggacgggaagcaggcgtgcgtgctcatgataaaagccctggagctcgac
agcaatctgtaccgcattggccagagcaaagtcttcttccgtgccggtgt
gctggcccacctggaggaggagcgagacctgaagatcaccgacgtcatca
tagggttccaggcctgctgcaggggctacctggccaggaaagcatttgcc
aagcggcagcagcagcttaccgccatgaaggtcctccagcggaactgcgc
tgcctacctgaagctgcggaactggcagtggtggcggctcttcaccaagg
tcaagccgctgctgcaggtgagccggcaggaggaggagatgatggccaag
gaggaggagctggtgaaggtcagagagaagcagctggctgcggagaacag
gctcacggagatggagacgctgcagtctcagctcatggcagagaaattgc
agctgcaggagcagctccaggcagaaaccgagctgtgtgccgaggctgag
gagctccgggcccgcctgaccgccaagaagcaggaattagaagagatctg
ccatgacctagaggccagggtggaggaggaggaggagcgctgccagcacc
tgcaggcggagaagaagaagatgcagcagaacatccaggagcttgaggag
cagctggaggaggaggagagcgcccggcagaagctgcagctggagaaggt
gaccaccgaggcgaagctgaaaaagctggaggaggagcagatcatcctgg
aggaccagaactgcaagctggccaaggaaaagaaactgctggaagacaga
atagctgagttcaccaccaacctcacagaagaggaggagaaatctaagag
cctcgccaagctcaagaacaagcatgaggcaatgatcactgacttggaag
agcgcctccgcagggaggagaagcagcgacaggagctggagaagacccgc
cggaagctggagggagactccacagacctcagcgaccagatcgccgagct
ccaggcccagatcgcggagctcaagatgcagctggccaagaaagaggagg
agctccaggccgccctggccagagtggaagaggaagctgcccagaagaac
atggccctcaagaagatccgggagctggaatctcagatctctgaactcca
ggaagacctggagtctgagcgtgcttccaggaataaagctgagaagcaga
aacgggaccttggggaagagctagaggcgctgaaaacagagttggaggac
acgctggattccacagctgcccagcaggagctcaggtcaaaacgtgagca
ggaggtgaacatcctgaagaagaccctggaggaggaggccaagacccacg
aggcccagatccaggagatgaggcagaagcactcacaggccgtggaggag
ctggcggagcagctggagcagacgaagcgggtgaaagcaaacctcgagaa
ggcaaagcagactctggagaacgagcggggggagctggccaacgaggtga
aggtgctgctgcagggcaaaggggactcggagcacaagcgcaagaaagtg
gaggcgcagctgcaggagctgcaggtcaagttcaacgagggagagcgcgt
gcgcacagagctggccgacaaggtcaccaagctgcaggtggagctggaca
acgtgaccgggcttctcagccagtccgacagcaagtccagcaagctcacc
aaggacttctccgcgctggagtcccagctgcaggacactcaggagctgct
gcaggaggagaaccggcagaagctgagcctgagcaccaagctcaagcagg
tggaggacgagaagaattccttccgggagcagctggaggaggaggaggag
gccaagcacaacctggagaagcagatcgccaccctccatgcccaggtggc
cgacatgaaaaagaagatggaggacagtgtggggtgcctggaaactgctg
aggaggtgaagaggaagctccagaaggacctggagggcctgagccagcgg
cacgaggagaaggtggccgcctacgacaagctggagaagaccaagacgcg
gctgcagcaggagctggacgacctgctggtggacctggaccaccagcgcc
agagcgcgtgcaacctggagaagaagcagaagaagtttgaccagctcctg
gcggaggagaagaccatctctgccaagtatgcagaggagcgcgaccgggc
tgaggcggaggcccgagagaaggagaccaaggctctgtcgctggcccggg
ccctggaggaagccatggagcagaaggcggagctggagcggctcaacaag
cagttccgcacggagatggaggaccttatgagctccaaggatgatgtggg
caagagtgtccacgagctggagaagtccaagcgggccctagagcagcagg
tggaggagatgaagacgcagctggaagagctggaggacgagctgcaggcc
accgaagatgccaagctgcggttggaggtcaacctgcaggccatgaaggc
ccagttcgagcgggacctgcagggccgggacgagcagagcgaggagaaga
agaagcagctggtcagacaggtgcgggagatggaggcagagctggaggac
gagaggaagcagcgctcgatggcagtggccgcccggaagaagctggagat
ggacctgaaggacctggaggcgcacatcgactcggccaacaagaaccggg
acgaagccatcaaacagctgcggaagctgcaggcccagatgaaggactgc
atgcgcgagctggatgacacccgcgcctctcgtgaggagatcctggccca
ggccaaagagaacgagaagaagctgaagagcatggaggccgagatgatcc
agttgcaggaggaactggcagccgcggagcgtgccaagcgccaggcccag
caggagcgggatgagctggctgacgagatcgccaacagcagcggcaaagg
agccctggcgttagaggagaagcggcgtctggaggcccgcatcgcccagc
tggaggaggagctggaggaggagcagggcaacacggagctgatcaacgac
cggctgaagaaggccaacctgcagatcgaccagatcaacaccgacctgaa
cctggagcgcagccacgcccagaagaacgagaatgctcggcagcagctgg
aacgccagaacaaggagcttaaggtcaagctgcaggagatggagggcact
gtcaagtccaagtacaaggcctccatcaccgccctcgaggccaagattgc
acagctggaggagcagctggacaacgagaccaaggagcgccaggcagcct
gcaaacaggtgcgtcggaccgagaagaagctgaaggatgtgctgctgcag
gtggatgacgagcggaggaacgccgagcagtacaaggaccaggccgacaa
ggcatctacccgcctgaagcagctcaagcggcagctggaggaggccgaag
aggaggcccagcgggccaacgcctcccgccggaaactgcagcgcgagctg
gaggacgccactgagacggccgatgccatgaaccgcgaagtcagctccct
aaagaacaagctcaggcgcggggacctgccgtttgtcgtgccccgccgaa
tggcccggaaaggcgccggggatggctccgacgaagaggtagatggcaaa
gcggatggggctgaggccaaacctgccgaataagcggccgc

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMAQQAADKYLYVDKNFINNPLAQADWAAKKLV
WVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSK
VEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSE
EIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTE
NTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNS
SRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLS
GAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIP
EEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINV
TDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLR
INKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNH
TMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEEC
WFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKAD
EWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETAL
PGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGK
LDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKG
FMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDV
IIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFT
KVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEK
LQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQ
HLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQII
LEDQNCKLAKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDL
EERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKE
EELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERASRNKAEK
QKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEAKT
HEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANE
VKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVEL
DNVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLK
QVEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVGCLET
AEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQ
RQSACNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLA
RALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQ
QVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEE
KKKQLVRQVREMEAELEDERKQRSMAVAARKKLEMDLKDLEAHIDSANKN
RDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEAEM
IQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIA
QLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQ
LERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQA
ACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEA
EEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPR
RMARKGAGDGSDEEVDGKADGAEAKPAE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11182
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 226522 Daltons