cDNA Clone NHE3

• Plasmid: pGEX6P-1-NHE3 D565-835(end)
• Parent Plasmid: pGEX6P1
• Expressed: GST-NHE3 D565-835(end)
• Genbank: NM_004174
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGACAACGTGGTCAACGTGGACTTCACGCCACGATCGTCCACCGTGGAGGCCTCTGTCTCCTACCTCCTGAGAGAAAATGTCAGCGCTGTCTGCCTGGACATGCAGTCTCTGGAGCAGCGACGGCGGAGCATCCGGGACGCGGAGGACATGGTCACGCACCACACGCTACAGCAGTACCTGTACAAGCCGCGGCAGGAGTACAAGCATCTGTACAGCCGACACGAGCTCACGCCCACGGAGGACGAGAAACAGGACCGGGAAATCTTCCACAGGACCATGCGGAAGCGCCTGGAGTCCTTC
AAGTCGACCAAGCTGGGGCTCAACCAGAACAAGAAGGCGGCCAAGCTGTA
CAAGCGGGAGCGTGCCCAGAAGCGGAGAAACAGCAGCATCCCCAATGGGA
AGCTGCCCATGGAGAGCCCTGCGCAGAATTTCACCATCAAGGAGAAAGAC
TTGGAACTTTCAGACACCGAGGAGCCCCCCAACTATGATGAGGAGATGAG
TGGGGGGATCGAGTTCCTGGCTAGTGTCACCAAGGACACAGCGTCCGACT
CCCCTGCAGGAATTGACAACCCTGTGTTTTCTCCGGACGAGGCCCTGGAC
CGCAGCCTCCTGGCCAGGCTGCCGCCCTGGCTGTCTCCCGGGGAGACGGT
GGTCCCCTCGCAGAGGGCCCGCACGCAGATTCCCTACTCTCCCGGCACCT
TCCGCCGCCTGATGCCCTTCCGCCTCAGCAGCAAGTCCGTGGACTCCTTC
CTGCAGGCAGACGGCCCCGAGGAGCGGCCCCCCGCCGCTCTCCCCGAGTC
CACACACATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDNVVNVDFTPRSSTVEASV
SYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKH
LYSRHELTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKR
ERAQKRRNSSIPNGKLPMESPAQNFTIKEKDLELSDTEEPPNYDEEMSGG
IEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLARLPPWLSPGETVVP
SQRARTQIPYSPGTFRRLMPFRLSSKSVDSFLQADGPEERPPAALPESTH
M*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11073
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences compared with NM_004174 CDS: a2031g, c2481t: both silent.; ; Estimated MW of expressed protein : 57730 Daltons