cDNA Clone APC4 (anaphase promoting complex subunit 4)

• Synonyms: ANAPC4
• Plasmid: pCMV5-HA APC4
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-APC4
• Genbank: NM_013367
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatgttgcgttttccgacctgtttcccatccttccgggtggtgggagagaagcagctcccgcaggagattattttcctggtctggtcgcccaagcgggatctcattgctttggccaacacagctggcgaggttttacttcatcgactggcaagttttcatcgagtttggagttttccaccaaatgaaaatacaggaaaggaggtgacgtgtctggcatggagaccagatggcaaacttttggcctttgctcttgctgataccaagaaaattgttttgtgtgatgtagaaaaacctgagagc
ttacactctttttctgtggaggctccagtttcctgtatgcattggatgga
agtgacagtagaaagcagtgttctcacatcattttataatgctgaggatg
aatcaaatcttctcttacctaaactacctacactgccaaaaaactatagc
aacacctcaaaaatatttagtgaagaaaattctgatgaaattattaagct
cttgggagacgtcaggcttaatattctcgtccttggaggaagctctggat
ttattgagctttatgcttatggaatgtttaaaattgctcgagtcacaggg
attgctggtacttgtcttgcattatgtttatcaagtgatttgaaatcatt
atcagtggtcacagaagtctctaccaatggtgcttcagaagtttcatact
ttcagcttgaaactaatctgttgtactctttcttacctgaagtaactcgg
atggccagaaagtttactcatatttcagctctgttacagtatataaattt
gtcactaacatgtatgtgtgaagcatgggaagaaatactaatgcagatgg
attctcgtctcaccaagtttgtgcaggaaaagaacacaaccacatcagtg
caagatgagttcatgcacttgctattatgggggaaagcaagtgctgaact
tcagactctcttgatgaatcagttaacagtaaagggcttgaaaaagcttg
gccagtctatagagtcatcatactccagtatacaaaaattggtcataagt
catttacagagtggctcggagtctttattataccatttgagtgaattgaa
aggaatggcttcatggaagcaaaaatatgaacctcttggactagatgctg
caggaatcgaagaagctataactgctgtgggttcttttatactcaaggca
aatgaacttcttcaagttatagatagtagtatgaaaaacttcaaagcatt
ttttcggtggctttatgtggcaatgttgagaatgacagaagaccatgtgc
ttcccgagctgaataagatgactcagaaagatatcacatttgttgctgaa
tttcttactgaacatttcaatgaggctccagacctttataatcgaaaagg
aaaatactttaacgttgaaagagttggtcagtacttgaaagatgaagatg
atgatcttgtgtcaccccctaacacagaaggaaaccagtggtatgacttt
cttcaaaatagcagccaccttaaagaaagtcctttgctgtttccttatta
tcctcgaaaatcattgcattttgtgaaaaggcggatggagaatattattg
atcagtgtttgcaaaagccagcagatgtaattggaaaatcgatgaatcaa
gcaatctgtattccattgtatagagataccagaagtgaggattctacacg
tagattgttcaaatttccttttctgtggaataataaaacttcaaatctac
attatcttctttttactattctagaagattcactttataaaatgtgcatc
ttaaggagacatactgatatttctcaatctgtgagtaatggactaattgc
tattaaatttgggagctttacatatgccacaacagaaaaagtcagaagaa
gcatctacagttgtttagatgcacagttttatgatgatgaaactgtaaca
gtagttcttaaagacactgtaggacgtgaaggaagagatagactcttggt
ccagctgcctttgtctttagtatataacagtgaagattctgcagaatatc
agttcactgggacttattctacaaggctagatgaacagtgtagtgctatt
cccacccgtaccatgcatttcgagaagcactggagattactggaaagtat
gaaagcacagtatgttgctgggaatggttttcgaaaagtgtcctgtgtgt
taagctcaaatcttcgtcatgtgagagtatttgaaatggacatagatgat
gaatgggagctcgatgagtcttcagatgaagaggaggaggccagtaataa
gcctgtaaaaataaaggaagaagtgttgtcggagtcagaggcagagaacc
aacaagctggtgctgccgctttagctccagagatagtcattaaagtggaa
aaacttgaccctgagctagactcctaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALA
NTAGEVLLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTK
KIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLL
LPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELY
AYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLET
NLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLT
KFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIE
SSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEE
AITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELN
KMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVS
PPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQ
KPADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLF
TILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSC
LDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGT
YSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNL
RHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGA
AALAPEIVIKVEKLDPELDS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11048
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_013367:; t2121c (silent F707); ; Derivative:; DU 11052 = pCMV5.HA APC4 F744A; ; ; Estimated MW of expressed protein : 93473 Daltons