cDNA Clone NICE-4

• Plasmid: pEGFP-C3-NICE-4
• Parent Plasmid: pEGFPC3
• Expressed: GFP-NICE-4
• Genbank: BC003170 (Q14157-2)
• Tag: GFP
• Species: Human

Sequence of Insert

CTCGAGATGATGACATCGGTGGGCACTAACCGAGCCCGGGGAAACTGGGAACAACCTCAAAACCAAAACCAGACACAGCACAAGCAGCGGCCACAGGCCACTGCAGAACAAATTAGACTTGCACAGATGATTTCGGACCATAATGATGCTGACTTTGAGGAGAAGGTGAAACAATTGATTGATATTACAGGCAAGAACCAGGATGAATGTGTGATTGCTTTGCATGACTGCAATGGAGATGTCAACAGAGCTATCAATGTTCTTCTGGAAGGAAACCCAGACACGCATTCCTGGGAGATGGTCGGG
AAGAAGAAGGGAGTCTCAGGCCAGAAGGATGGTGGCCAGACGGAATCCAA
TGAGGAAGGCAAAGAAAATCGAGACCGGGACAGAGACTATAGTCGGCGAC
GTGGTGGGCCACCAAGACGGGGGAGAGGTGCCAGCCGTGGACGAGAGTTT
CGAGGTCAGGAAAATGGATTGGATGGCACCAAGAGTGGAGGGCCTTCTGG
AAGAGGAACAGAAAGAGGCAGAAGGGGCCGTGGCCGAGGCAGAGGTGGCT
CTGGTAGGCGAGGAGGAAGGTTTTCTGCTCAAGGAATGGGAACCTTTAAC
CCAGCTGATTATGCAGAGCCAGCCAATACTGATGATAACTATGGCAATAG
CAGCGGCAATACGTGGAACAACACTGGCCACTTTGAACCAGATGATGGGA
CGAGTGCATGGAGGACTGCAACAGAGGAGTGGGGGACTGAAGATTGGAAT
GAAGATCTTTCTGAGACCAAGATCTTCACTGCCTCTAATGTGTCTTCAGT
GCCTCTGCCTGCGGAGAATGTGACAATCACTGCTGGTCAGAGAATTGACC
TTGCTGTTCTGCTGGGGAAGACACCATCTACAATGGAGAATGATTCATCT
AATCTGGATCCGTCTCAGGCTCCTTCTCTGGCCCAGCCTCTGGTGTTCAG
TAATTCGAAGCAGACTGCCATATCACAGCCTGCTTCAGGGAACACATTTT
CTCATCACAGTATGGTGAGCATGTTAGGGAAAGGATTTGGTGATGTCGGT
GAAGCTAAAGGCGGCAGTACTACAGGCTCCCAGTTCTTGGAGCAATTCAA
GACTGCCCAAGCCCTGGCTCAGTTGGCAGCTCAGCATTCTCAGTCTGGAA
GCACCACCACCTCCTCTTGGGACATGGGCTCGACGACACAATCCCCATCA
CTGGTGCAGTATGATTTGAAGAACCCAAGTGATTCAGCAGTGCACAGCCC
CTTTACAAAGCGCCAGGCTTTTACCCCATCTTCAACCATGATGGAGGTGT
TCCTTCAGGAGAAGTCACCTGCAGTGGCTACCTCCACAGCTGCACCTCCA
CCTCCGTCTTCTCCTCTGCCAAGCAAATCCACATCGGCTCCACAGATGTC
GCCTGGATCTTCAGACAACCAGTCCTCTAGCCCTCAGCCGGCTCACCAGA
AACTGAAACAGCAGAAGAAAAAAGCCTCCTTGACTTCTAAGATTCCTGCT
CTGGCTGTGGAGATGCCTGGCTCAGCAGATATCTCAGGGCTAAACCTGCA
GTTTGGGGCATTGCAGTTTGGGTCAGAGCCTGTCCTTTCTGATTATGAGT
CCACCCCCACCACGAGCGCCTCTTCAAGCCAGGCTCCAAGTAGCCTGTAT
ACCAGCACGGCCAGTGAATCATCCTCTACAATTTCATCTAACCAGAGTCA
GGAGTCTGGTTATCAGAGCGGCCCAATTCAGTCGACAACCTATACCTCCC
AAAATAATGCTCAGGGCCCTCTTTATGAACAGAGATCCACACAGACTCGG
CGGTACCCCAGCTCCATCTCTTCATCACCCCAAAAGGACCTGACTCAGGC
AAAGAATGGCTTCAGTTCTGTGCAGGCCACGCAGTTACAGACCACACAAT
CTGTTGAAGGTGCTACAGGCTCTGCAGTGAAATCTGATTCACCTTCCACT
TCTAGCATCCCCCCTCTCAATGAAACGGTATCTGCAGCTTCCTTACTGAC
GACAACCAATCAGCATTCATCCTCCTTGGGTGGCTTGAGCCACAGTGAGG
AGATTCCAAATACTACCACCACACAACACAGCAGCACGTTATCTACGCAG
CAGAATACCCTTTCATCATCAACATCTTCTGGGCGCACTTCGACATCCAC
TCTTTTGCACACAAGTGTGGAGAGTGAGGCGAATCTCCATTCTTCCTCCA
GCACTTTTTCCACCACATCCAGCACAGTCTCTGCACCTCCCCCAGTGGTC
AGTGTCTCCTCCAGTCTCAATAGTGGCAGTAGCCTGGGCCTCAGCCTAGG
CAGCAACTCCACTGTCACAGCCTCGACTCGAAGCTCAGTTGCTACGACTT
CAGGAAAAGCTCCTCCCAACCTCCCTCCTGGGGTCCCGCCGTTGTTGCCT
AATCCGTATATTATGGCTCCAGGGCTGTTACATGCCTACCCGCCACAAGT
ATATGGTTATGATGACTTGCAGATGCTTCAGACAAGATTTCCATTGGATT
ACTACAGCATCCCATTTCCCACACCCACTACTCCGCTGACTGGGAGGGAT
GGTAGCCTGGCCAGCAACCCTTATTCTGGTGACCTCACAAAGTTCGGCCG
TGGGGATGCCTCCTCCCCAGCCCCGGCCACAACCTTGGCCCAACCCCAAC
AGAACCAGACGCAGACTCACCATACCACGCAGCAGACATTCCTGAACCCG
GCGCTGCCTCCTGGCTACAGTTACACCAGCCTGCCATACTATACAGGGGT
CCCGGGCCTCCCCAGCACCTTCCAGTATGGGCCTGCTGTGTTCCCTGTGG
CTCCTACCTCTTCCAAGCAGCATGGTGTGAATGTCAGTGTGAATGCATCG
GCCACCCCTTTCCAACAGCCGAGTGGATATGGGTCTCATGGATACAACAC
TGGTGTTTCAGTCACCTCCAGTAACACGGGCGTGCCAGATATCTCGGGTT
CTGTGTACTCCAAAACCCAGCAGTCCTTTGAGAAACAAGGTTTTCATTCC
GGTACTCCTGCTGCTTCCTTCAACTTGCCTTCAGCCCTAGGAAGTGGGGG
CCCCATCAATCCGGCCACAGCTGCTGCCTACCCACCTGCCCCCTTTATGC
ACATTCTGACCCCCCATCAGCAGCCGCATTCTCAGATCCTTCACCATCAC
CTGCAGCAGGATGGCCAGACGGGCAGCGGGCAACGTAGCCAGACCAGCTC
CATCCCGCAGAAGCCCCAGACCAACAAGTCTGCCTACAACAGCTACAGCT
GGGGGGCCAACTGAAAGCTT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKYSDLEMMTSVG
TNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQ
LIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVS
GQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENG
LDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAE
PANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSET
KIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQ
APSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGS
TTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDL
KNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPL
PSKSTSAPQMSPGSSDNQSSSPQPAHQKLKQQKKKASLTSKIPALAVEMP
GSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASE
SSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSI
SSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPL
NETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSS
STSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSL
NSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMA
PGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASN
PYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGY
SYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQ
PSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAAS
FNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILHHHLQQDGQ
TGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11037
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression construct. N-terminal GFP tag.