cDNA Clone NIA2 (Aribadopsis)

• Plasmid: pCMV5-HA3 At NIA2
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-At NIA2
• Genbank: NM_103364
• Tag: HA
• Species: Arabidopsis thaliana

Sequence of Insert

gtcgacatggcggcctctgtagataatcgccaatacgctcgtctcgagccaggtttgaacggcgtggttcgttcttacaaacctcccgttccaggccggtctgattcccctaaggcgcaccagaaccaaaccaccaaccaaaccgtgttcttgaaaccagccaaggttcatgacgatgacgaagacgtgtcgagcgaagacgagaacgagacacacaacagcaacgccgtgtactacaaggagatgataagaaaatccaacgccgagcttgaaccgtccgttttggacccgagggacgaatacacg
gctgatagctggatcgagcgtaacccttccatggtacgtctcacagggaa
acatcccttcaactccgaggcgcctcttaaccgtttaatgcaccacgggt
ttatcacccctgtcccgttgcactacgttcgtaaccacggccacgtccct
aaagcccaatgggccgaatggacggtcgaggtgaccggattcgtcaaacg
gcccatgaaattcaccatggaccagctcgtctccgagtttgcttaccgcg
agttcgccgcgacgctagtctgcgcggggaaccgccgtaaggaacagaac
atggtgaagaagtcaaagggattcaactggggatccgccggagtttccac
ctccgtgtggcgtggtgtccctctctgcgacgtactgcgtcgctgcggga
tctttagccgaaaaggcggcgctctcaacgtctgcttcgaagggtcggag
gatcttccgggcggtgccggaactgctggttccaaatacggaacgagcat
caagaaggaatatgccatggatccatcaagagacatcattttggcttata
tgcaaaacggagagtatctaacaccagaccacggttttccggttcggatc
atcatccccggtttcattggtggccggatggttaaatggttgaaacgaat
cattgtcacaactaaagaatccgacaatttctaccatttcaaggacaaca
gagttttaccttctttggtagacgccgaactcgccgacgaagaaggttgg
tggtataagccagagtacataatcaacgagctaaacataaactccgtgat
tacgacgccatgtcacgaggagattcttcccatcaacgctttcacaaccc
aaagaccttatactttaaagggttacgcatattccggaggtggaaaaaaa
gtgacccgtgtggaggtcacggtagatggtggagagacatggaacgtatg
tgcacttgaccatcaagagaagccaaacaagtatgggaagttctggtgtt
ggtgtttttggtcacttgaggttgaggttttggacttgcttagtgccaaa
gagattgctgttcgtgcatgggacgagactctcaacacgcagcccgagaa
aatgatatggaatctcatggggatgatgaataactgctggtttagagtga
agactaacgtgtgcaagccacacaagggagagattgggattgtgttcgag
catccaacgcttcctggtaatgaatctggtggatggatggcgaaggaacg
tcacctcgaaaaatcggctgacgcgcctcctagtctaaagaagtctgtct
cgacgccgtttatgaacacaactgcgaagatgtactcgatgtccgaggtc
aagaagcataattcggctgactcttgctggatcattgtccatggacatat
ctatgattgtacacgattccttatggatcacccgggtggttcggattcaa
tcttgatcaatgctggtacggattgtacggaggagtttgaagccattcac
tcggataaagccaagaagatgcttgaggattaccgtatcggtgagctcat
caccactggttattcctctgactcttcctcgcctaacaactcggttcacg
gttcatccgccgtgttctcgctgttggctcccattggagaggcgactccg
gttaggaacctcgctttggttaatccccgggctaaagtcccggttcaact
cgtcgaaaagacttccatttctcatgatgttcgtaaattccggtttgctt
taccggttgaggatatggttctaggcttaccggttggtaagcacattttc
ctttgcgccaccatcaatgacaagctctgcctcagagcttacacgccaag
cagcaccgttgatgtggttggctacttcgagctcgtggtcaagatttact
ttggcggtgtccacccaagattccctaacggcgggctcatgtctcagtac
ctagactctttgcctatagggtcaactttggagattaaaggaccattggg
tcacgttgagtatctcggcaagggtagtttcacggttcacggtaaaccaa
agtttgctgataaattggcaatgttggcaggtggaaccggaataactccg
gtttaccaaattatccaagccattctcaaggatccagaggatgagactga
aatgtacgtcatttatgctaaccggaccgaggaagatattctcctaaggg
aggaactggatggttgggcagagcaatacccggaccggttaaaggtttgg
tacgtagtggaatcagctaaggaaggttgggcatacagtaccgggtttat
ttccgaggcgattatgcgagaacatatccctgatggattagatggctcag
cccttgccatggcttgcggaccaccaccgatgattcagtttgcggttcag
ccgaatttggagaagatgcaatataacatcaaggaggatttcttgatatt
ctaggcggccgc

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMAASVDNRQYARLEPGLNGVVRSYKPPVPGR
SDSPKAHQNQTTNQTVFLKPAKVHDDDEDVSSEDENETHNSNAVYYKEMI
RKSNAELEPSVLDPRDEYTADSWIERNPSMVRLTGKHPFNSEAPLNRLMH
HGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKFTMDQLVSEFA
YREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVLRR
CGIFSRKGGALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIIL
AYMQNGEYLTPDHGFPVRIIIPGFIGGRMVKWLKRIIVTTKESDNFYHFK
DNRVLPSLVDAELADEEGWWYKPEYIINELNINSVITTPCHEEILPINAF
TTQRPYTLKGYAYSGGGKKVTRVEVTVDGGETWNVCALDHQEKPNKYGKF
WCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKMIWNLMGMMNNCWF
RVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPSLKK
SVSTPFMNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGS
DSILINAGTDCTEEFEAIHSDKAKKMLEDYRIGELITTGYSSDSSSPNNS
VHGSSAVFSLLAPIGEATPVRNLALVNPRAKVPVQLVEKTSISHDVRKFR
FALPVEDMVLGLPVGKHIFLCATINDKLCLRAYTPSSTVDVVGYFELVVK
IYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHG
KPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDIL
LREELDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLD
GSALAMACGPPPMIQFAVQPNLEKMQYNIKEDFLIF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11021
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with NM_103364 CDS:; c96t, a2145g (both silent); ; ; Estimated MW of expressed protein : 105053 Daltons