cDNA Clone FKHRL1

• Plasmid: pEBG-2T-FKHR L1
• Parent Plasmid: pEBG2T
• Expressed: GST-FKHRL1
• Genbank: NM_001455
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCAGAGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGT
GGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGTCCGCGATCCTGTACGT
GGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCCTCGGGG
GAGACGGCCGCCGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGA
CGAGGACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCG
GCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGG
GTGCTGGCACCCGGAGGGCAAGACCCCGGGTCTGGGCCAGCCACCGCGGC
GGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTGCAGCCTCAGCAACCGC
TGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCGAGGAAA
TGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGAT
CACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGA
TCTACGAGTGGATGGTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGAC
AGCAACAGCTCTGCCGGCTGGAAGAACTCCATCCGGCACAACCTGTCACT
GCATAGTCGATTCATGCGGGTCCAGAATGAGGGAACTGGCAAGAGCTCTT
GGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCCCGGCGG
CGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCG
CGCAGCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACG
ACAGTCCCTCCCAGCTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGC
AGTGATGAGCTGGATGCGTGGACGGACTTCCGTTCACGCACCAATTCTAA
CGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATCATGGCAAGCACAGAGT
TGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTCTACAGC
AGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACT
GCCACGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGA
CTGAAAACCTCATGGACGACCTGCTGGATAACATCACGCTCCCGCCATCC
CAGCCATCGCCCACTGGGGGACTCATGCAGCGGAGCTCTAGCTTCCCGTA
TACCACCAAGGGCTCGGGCCTGGGCTCCCCAACCAGCTCCTTTAACAGCA
CGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCCATGCAG
ACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGG
TAACCAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCG
ATGTCATGATGACACAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCT
GTGTCTGCCCAGAATTCCCGCCGGAACGTGATGCTTCGCAATGATCCGAT
GATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGTTTGGTCAATCAGAACT
TGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCC
TTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGG
GTCAGCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCC
TCTCGGACTCTCTCTCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTG
CCCGTCATGGGCCATGAGAAGTTCCCCAGCGACTTGGACCTGGACATGTT
CAATGGGAGCTTGGAATGTGACATGGAGTCCATTATCCGTAGTGAACTCA
TGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCCACACAG
AATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTC
ATCTCAGAGCTGGGTGCCAGGCTGAGTCGACAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1072
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag; Change to NM_001455 orf sequence:; t159c (silent in A53) – natural variant