cDNA Clone MLCK

• Plasmid: pGEX6P-1-smMLCK
• Parent Plasmid: pGEX6P1
• Expressed: GST-smMLCK
• Genbank: NM_005965
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGT
GTCTGAGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCT
CTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTG
CCACCGCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGCGGCAA
GAAGAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATG
CCAAGGCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGG
CCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAAT
GGGCAACGCCAAGCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGC
CTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTT
AAGAATGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGA
AAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGC
AAGATGTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTG
TCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAATGGAAAGACCCT
CAAGACCACCAAGTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCG
TCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTA
GCCAAGAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGA
TGATGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGA
GGCCCAAGAGCTCTCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACT
GTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCC
TCAGATCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAG
TGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGTACCTGG
ATGAAGTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAA
CAGCGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACT
GCGGCTGCTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCC
CAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACC
TTGTGCCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCT
CCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGG
GACTCAGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTC
TTTCAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTAC
GTGCAATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTC
ACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTC
AGATGATGATGAGAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCA
ATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGATTAGGA
TCTGGGAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAA
AGTCTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGA
ATATCCGGCAGGAGATTAGCATCATGAACTGCCTCCACCACCCTAAGCTG
GTCCAGTGTGTGGATGCCTTTGAAGAAAAGGCCAACATCGTCATGGTCCT
GGAGATCGTGTCAGGAGGGGAGCTGTTTGAGCGCATCATTGACGAGGACT
TTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAG
GGAGTGGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCC
GGAGAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCG
ACTTTGGTCTGGCCAGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTC
TTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGATCAACTATGAGCCCAT
CGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTACATCCTAG
TCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACCTTGGCC
AACGTTACCTCAGCCACCTGGGACTTCGACGACGAGGCATTCGATGAGAT
CTCCGACGATGCCAAGGATTTCATCAGCAATCTGCTGAAGAAAGATATGA
AAAACCGCCTGGACTGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAA
GATACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAA
GTACATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCA
TTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAA
TCCTCAACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATC
TGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTC
ATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAG
GGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGA
GGTTGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCC
AGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTT
TGCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGG
AGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTG
AAGGGGAAGGGGAAGAGGAAGAAGAGTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVPPPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLESEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1065
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; Changes to NM_005965 ORF sequence: ; t192c (silent in G64), c1551t (silent in D517), c2076t (silent in N692).