cDNA Clone NFkB p105

• Plasmid: pEBG-2T-p105
• Parent Plasmid: pEBG2T
• Expressed: GST-p105
• Genbank: M55643
• Tag: GST
• Species: Human

Sequence of Insert

ggtaccGCAGAAGATGATCCATATTTGGGAAGGCCTGAACAAATGTTTCA
TTTGGATCCTTCTTTGACTCATACAATATTTAATCCAGAAGTATTTCAAC
CACAGATGGCACTGCCAACAGCAGATGGCCCATACCTTCAAATATTAGAG
CAACCTAAACAGAGAGGATTTCGTTTCCGTTATGTATGTGAAGGCCCATC
CCATGGTGGACTACCTGGTGCCTCTAGTGAAAAGAACAAGAAGTCTTACC
CTCAGGTCAAAATCTGCAACTATGTGGGACCAGCAAAGGTTATTGTTCAG
TTGGTCACAAATGGAAAAAATATCCACCTGCATGCCCACAGCCTGGTGGG
AAAACACTGTGAGGATGGGATCTGCACTGTAACTGCTGGACCCAAGGACA
TGGTGGTCGGCTTCGCAAACCTGGGTATACTTCATGTGACAAAGAAAAAA
GTATTTGAAACACTGGAAGCACGAATGACAGAGGCGTGTATAAGGGGCTA
TAATCCTGGACTCTTGGTGCACCCTGACCTTGCCTATTTGCAAGCAGAAG
GTGGAGGGGACCGGCAGCTGGGAGATCGGGAAAAAGAGCTAATCCGCCAA
GCAGCTCTGCAGCAGACCAAGGAGATGGACCTCAGCGTGGTGCGGCTCAT
GTTTACAGCTTTTCTTCCGGATAGCACTGGCAGCTTCACAAGGCGCCTGG
AACCCGTGGTATCAGACGCCATCTATGACAGTAAAGCCCCCAATGCATCC
AACTTGAAAATTGTAAGAATGGACAGGACAGCTGGATGTGTGACTGGAGG
GGAGGAAATTTATCTTCTTTGTGACAAAGTTCAGAAAGATGACATCCAGA
TTCGATTTTATGAAGAGGAAGAAAATGGTGGAGTCTGGGAAGGATTTGGA
GATTTTTCCCCCACAGATGTTCATAGACAATTTGCCATTGTCTTCAAAAC
TCCAAAGTATAAAGATATTAATATTACAAAACCAGCCTCTGTGTTTGTCC
AGCTTCGGAGGAAATCTGACTTGGAAACTAGTGAACCAAAACCTTTCCTC
TACTATCCTGAAATCAAAGATAAAGAAGAAGTGCAGAGGAAACGTCAGAA
GCTCATGCCCAATTTTTCGGATAGTTTCGGCGGTGGTAGTGGTGCCGGAG
CTGGAGGCGGAGGCATGTTTGGTAGTGGCGGTGGAGGAGGGGGCACTGGA
AGTACAGGTCCAGGGTATAGCTTCCCACACTATGGATTTCCTACTTATGG
TGGGATTACTTTCCATCCTGGAACTACTAAATCTAATGCTGGGATGAAGC
ATGGAACCATGGACACTGAATCTAAAAAGGACCCTGAAGGTTGTGACAAA
AGTGATGACAAAAACACTGTAAACCTCTTTGGGAAAGTTATTGAAACCAC
AGAGCAAGATCAGGAGCCCAGCGAGGCCACCGTTGGGAATGGTGAGGTCA
CTCTAACGTATGCAACAGGAACAAAAGAAGAGAGTGCTGGAGTTCAGGAT
AACCTCTTTCTAGAGAAGGCTATGCAGCTTGCAAAGAGGCATGCCAATGC
CCTTTTCGACTACGCGGTGACAGGAGACGTGAAGATGCTGCTGGCCGTCC
AGCGCCATCTCACTGCTGTGCAGGATGAGAATGGGGACAGTGTCTTACAC
TTAGCAATCATCCACCTTCATTCTCAACTTGTGAGGGATCTACTAGAAGT
CACATCTGGTTTGATTTCTGATGACATTATCAACATGAGAAATGATCTGT
ACCAGACGCCCTTGCACTTGGCAGTGATCACTAAGCAGGAAGATGTGGTG
GAGGATTTGCTGAGGGCTGGGGCCGACCTGAGCCTTCTGGACCGCTTGGG
TAACTCTGTTTTGCACCTAGCTGCCAAAGAAGGACATGATAAAGTTCTCA
GTATCTTACTCAAGCACAAAAAGGCAGCACTACTTCTTGACCACCCCAAC
GGGGACGGTCTGAATGCCATTCATCTAGCCATGATGAGCAATAGCCTGCC
ATGTTTGCTGCTGCTGGTGGCCGCTGGGGCTGACGTCAATGCTCAGGAGC
AGAAGTCCGGGCGCACAGCACTGCACCTGGCTGTGGAGCACGACAACATC
TCATTGGCAGGCTGCCTGCTCCTGGAGGGTGATGCCCATGTGGACAGTAC
TACCTACGATGGAACCACACCCCTGCATATAGCAGCTGGGAGAGGGTCCA
CCAGGCTGGCAGCTCTTCTCAAAGCAGCAGGAGCAGATCCCCTGGTGGAG
AACTTTGAGCCTCTCTATGACCTGGATGACTCTTGGGAAAATGCAGGAGA
GGATGAAGGAGTTGTGCCTGGAACCACGCCTCTAGATATGGCCACCAGCT
GGCAGGTATTTGACATATTAAATGGGAAACCATATGAGCCAGAGTTTACA
TCTGATGATTTACTAGCACAAGGAGACATGAAACAGCTGGCTGAAGATGT
GAAGCTGCAGCTGTATAAGTTACTAGAAATTCCTGATCCAGACAAAAACT
GGGCTACTCTGGCGCAGAAATTAGGTCTGGGGATACTTAATAATGCCTTC
CGGCTGAGTCCTGCTCCTTCCAAAACACTTATGGACAACTATGAGGTCTC
TGGGGGTACAGTCAGAGAGCTGGTGGAGGCCCTGAGACAAATGGGCTACA
CCGAAGCAATTGAAGTGATCCAGGCAGCCTCCAGCCCAGTGAAGACCACC
TCTCAGGCCCACTCGCTGCCTCTCTCGCCTGCCTCCACAAGGCAGCAAAT
AGACGAGCTCCGAGACAGTGACAGTGTCTGCGACAGCGGCGTGGAGACAT
CCTTCCGCAAACTCAGCTTTACCGAGTCTCTGACCAGTGGTGCCTCACTG
CTAACTCTCAACAAAATGCCCCATGATTATGGGCAGGAAGGACCTCTAGA
AGGCAAAATTTAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSGTAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHSQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKVLSILLKHKKAALLLDHPNGDGLNAIHLAMMSNSLPCLLLLVAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1047
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag; Changes to M55643 sequence: c2783g and g2784c resulting in residue change T928S (present in all available EST sequences and some mRNA sequences eg AF213884 and AY223820).