cDNA Clone NFkB C-Rel

• Plasmid: pFastBac.HTb C-Rel
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-C-Rel
• Genbank: X75042
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCTCCGGTGCGTATAACCCGTATATAGAGATAATTGAACA
ACCCAGGCAGAGGGGAATGCGTTTTAGATACAAATGTGAAGGGCGATCAG
CAGGCAGCATTCCAGGGGAGCACAGCACAGACAACAACCGAACATACCCT
TCTATCCAGATTATGAACTATTATGGAAAAGGAAAAGTGAGAATTACATT
AGTAACAAAGAATGACCCATATAAACCTCATCCTCATGATTTAGTTGGAA
AAGACTGCAGAGACGGCTACTATGAAGCAGAATTTGGACAAGAACGCAGA
CCTTTGTTTTTCCAAAATTTGGGTATTCGATGTGTGAAGAAAAAAGAAGT
AAAAGAAGCTATTATTACAAGAATAAAGGCAGGAATCAATCCATTCAATG
TCCCTGAAAAACAGCTGAATGATATTGAAGATTGTGACCTCAATGTGGTG
AGACTGTGTTTTCAAGTTTTTCTCCCTGATGAACATGGTAATTTGACGAC
TGCTCTTCCTCCTGTTGTCTCGAACCCAATTTATGACAACCGTGCTCCAA
ATACTGCAGAATTAAGGATTTGTCGTGTAAACAAGAATTGTGGAAGTGTC
AGAGGAGGAGATGAAATATTTCTACTTTGTGACAAAGTTCAGAAAGATGA
CATAGAAGTTCGTTTTGTGTTGAACGATTGGGAAGCAAAAGGCATCTTTT
CACAAGCTGATGTACACCGTCAAGTAGCCATTGTTTTCAAAACTCCACCA
TATTGCAAAGCTATCACAGAACCCGTAACAGTAAAAATGCAGTTGCGGAG
ACCTTCTGACCAGGAAGTTAGTGAATCTATGGATTTTAGATATCTGCCAG
ATGAAAAAGATACTTACGGCAATAAAGCAAAGAAACAAAAGACAACTCTG
CTTTTCCAGAAACTGTGCCAGGATCACGTTAATTTTCCTGAGAGACCAAG
ACCTGGTCTCCTCGGTTCAATTGGAGAAGGAAGATACTTCAAAAAAGAAC
CAAACCTGTTTTCTCATGATGCAGTTGTGAGAGAAATGCCTACAGGGGTT
TCAAGTCAAGCAGAATCCTACTATCCCTCACCTGGGCCCATCTCAAGTGG
ATTGTCACATCATGCCTCAATGGCACCTCTGCCTTCTTCAAGCTGGTCAT
CAGTGGCCCACCCCACCCCACGCTCAGGCAATACAAACCCACTGAGTAGT
TTTTCAACAAGGACACTTCCTTCTAATTCGCAAGGTATCCCACCATTCCT
GAGAATACCTGTTGGGAATGATTTAAATGCTTCTAATGCTTGCATTTACA
ACAATGCCGATGACATAGTCGGAATGGAAGCGTCATCCATGCCATCAGCA
GATTTATATGGTATTTCTGATCCCAACATGATGTCTAATTGTTCTGTGAA
TATGATGACAACCAGCAGTGACAGCATGGGAGAGACTGATAATCCAAGAC
TTCTGAGCATGAATCTTGAAAACCCCTCATGTAATTCAGTGTTAGACCCA
AGAGACTTGAGACAGCTCCATCAGATGTCCTCTTCCAGTATGTCAGCAGG
CGCCAATTCCAATACTACTGTTTTTGTTTCACAATCAGATGCATTTGAGG
GATCTGACTTCAGTTGTGCAGATAACAGCATGATAAATGAGTCGGGACCA
TCAAACAGTACTAATCCAAACAGTCATGGTTTTGTTCAAGATAGTCAGTA
TTCAGGTATTGGCAGTATGCAAAATGAGCAATTGAGTGACTCCTTTCCAT
ATGAATTTTTTCAAGTATAAGGTACC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSASGAYNPYIEIIEQPRQRGMRFRYKCEGRSAGSIPGEHSTDNNRTYPSIQIMNYYGKGKVRITLVTKNDPYKPHPHDLVGKDCRDGYYEAEFGQERRPLFFQNLGIRCVKKKEVKEAIITRIKAGINPFNVPEKQLNDIEDCDLNVVRLCFQVFLPDEHGNLTTALPPVVSNPIYDNRAPNTAELRICRVNKNCGSVRGGDEIFLLCDKVQKDDIEVRFVLNDWEAKGIFSQADVHRQVAIVFKTPPYCKAITEPVTVKMQLRRPSDQEVSESMDFRYLPDEKDTYGNKAKKQKTTLLFQKLCQDHVNFPERPRPGLLGSIGEGRYFKKEPNLFSHDAVVREMPTGVSSQAESYYPSPGPISSGLSHHASMAPLPSSSWSSVAHPTPRSGNTNPLSSFSTRTLPSNSQGIPPFLRIPVGNDLNASNACIYNNADDIVGMEASSMPSADLYGISDPNMMSNCSVNMMTTSSDSMGETDNPRLLSMNLENPSCNSVLDPRDLRQLHQMSSSSMSAGANSNTTVFVSQSDAFEGSDFSCADNSMINESGPSNSTNPNSHGFVQDSQYSGIGSMQNEQLSDSFPYEFFQV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1025
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; Changes to published sequence: t1096c (L366L), c1471a (L491M), deletion of published orf nts 922-1017 (32 amino acid exon not found in any ESTs) (Brownell, E et al , Oncogene 1989, 4, p935-42)