cDNA Clone NFkB p50

• Plasmid: pGEX6P-1-p50
• Parent Plasmid: pGEX6P1
• Expressed: GST-p50
• Genbank: M55643
• Tag: GST
• Species: Human

Sequence of Insert

gaattcGCAGAAGATGATCCATATTTGGGAAGGCCTGAACAAATGTTTCA
TTTGGATCCTTCTTTGACTCATACAATATTTAATCCAGAAGTATTTCAAC
CACAGATGGCACTGCCAACAGCAGATGGCCCATACCTTCAAATATTAGAG
CAACCTAAACAGAGAGGATTTCGTTTCCGTTATGTATGTGAAGGCCCATC
CCATGGTGGACTACCTGGTGCCTCTAGTGAAAAGAACAAGAAGTCTTACC
CTCAGGTCAAAATCTGCAACTATGTGGGACCAGCAAAGGTTATTGTTCAG
TTGGTCACAAATGGAAAAAATATCCACCTGCATGCCCACAGCCTGGTGGG
AAAACACTGTGAGGATGGGATCTGCACTGTAACTGCTGGACCCAAGGACA
TGGTGGTCGGCTTCGCAAACCTGGGTATACTTCATGTGACAAAGAAAAAA
GTATTTGAAACACTGGAAGCACGAATGACAGAGGCGTGTATAAGGGGCTA
TAATCCTGGACTCTTGGTGCACCCTGACCTTGCCTATTTGCAAGCAGAAG
GTGGAGGGGACCGGCAGCTGGGAGATCGGGAAAAAGAGCTAATCCGCCAA
GCAGCTCTGCAGCAGACCAAGGAGATGGACCTCAGCGTGGTGCGGCTCAT
GTTTACAGCTTTTCTTCCGGATAGCACTGGCAGCTTCACAAGGCGCCTGG
AACCCGTGGTATCAGACGCCATCTATGACAGTAAAGCCCCCAATGCATCC
AACTTGAAAATTGTAAGAATGGACAGGACAGCTGGATGTGTGACTGGAGG
GGAGGAAATTTATCTTCTTTGTGACAAAGTTCAGAAAGATGACATCCAGA
TTCGATTTTATGAAGAGGAAGAAAATGGTGGAGTCTGGGAAGGATTTGGA
GATTTTTCCCCCACAGATGTTCATAGACAATTTGCCATTGTCTTCAAAAC
TCCAAAGTATAAAGATATTAATATTACAAAACCAGCCTCTGTGTTTGTCC
AGCTTCGGAGGAAATCTGACTTGGAAACTAGTGAACCAAAACCTTTCCTC
TACTATCCTGAAATCAAAGATAAAGAAGAAGTGCAGAGGAAACGTCAGAA
GCTCATGCCCAATTTTTCGGATAGTTTCGGCGGTGGTAGTGGTGCCGGAG
CTGGAGGCGGAGGCATGTTTGGTAGTGGCGGTGGAGGAGGGGGCACTGGA
AGTACAGGTCCAGGGTATAGCTTCCCACACTATGGATTTCCTACTTATGG
TGGGATTACTTTCCATCCTGGAACTACTAAATCTAATGCTGGGATGAAGC
ATTGAgtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1006
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion