cDNA Clone NKCC1 (mouse)

• Plasmid: PRK5-NKCC1 (mouse) no kozak (R1 insert)
• Parent Plasmid: pRK5
• Expressed: NKCC1 (mouse)
• Genbank: NM_009194_
• Species: Mouse

Sequence of Insert

ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGGCTGCCGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTGCCCTCCGGCCAAGAGGATGCTACTACGGCTGGGAGGCAGGCTGGCGGCGGGGTGCGCGGAGAGGGAACCCCAGCGGCGGGCGATGGGCTGGGCAGACCTCTGGGGCCCACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCGGCTGCGGCGGCGGCGGCGGCGGCTGCGGCTGCGGCCGGGGCGGCGGGCAAGGAGACCCCCGCGGCCGGGAAAGCCGGCGGTGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTGGACCCGGCTGCCTCGTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGGGTCGGTGGCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGGGGTGGCAGTGGACACCACCAGCAGTACTACTATGACACCCACACCAACACCTACTACCTGCGCACCTTCGGCCACAACACCATGGACGCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCCAGCCTGGCAGAGCTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCGAATGGAGAAGAAAGTACTCCAACCAGAGATGCTGTGGTCGCATACACTGCCGAGAGTAAAGGAGTTGTAAAGTTTGGCTGGATCAAGGGTGTATTAGTCCGCTGCATGTTAAACATTTGGGGTGTGATGCTCTTTATTAGATTGTCATGGATCGTGGGTCAAGCTGGGATAGGTCTCTCTGTCGTCGTAATAGCAATGGCCACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCTACCAATGGGTTCGTGAGAGGAGGAGGAGCGTACTATTTAATCTCTAGAAGTCTAGGGCCGGAATTTGGTGGTGCAATTGGCTTGATCTTTGCTTTTGCCAATGCGGTGGCCGTTGCTATGTATGTTGTCGGATTCGCAGAGACTGTGGTGGAGCTGCTTAAGGAACATTCCATACTTATGATAGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTCTGGGCATCTCAGTGGCTGGAATGGAGTGGGAAGCAAAGGCTCAGATTGTGCTGCTGGTGATCCTTCTCCTGGCCATCGCCGACTTCGTCATAGGGACGTTTATCTCACTGGAGAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATTTAATGAGAACTTTGGGCCGGATTTTCGAGAAGAAGAGACTTTCTTCTCTGTATTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTAGCTGGAGCAAATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACACTCTTAGCCATTTTGATTACTACAGTGGTTTACATAGGAATTGCAGTGTCTGTGGGTTCGTGTGTTGTTCGGGATGCCACTGGCAATGTTAATGATACCATTACAACAGAGCTGACAAACTGTACTTCTGCAGCCTGCAAACTAAACTTTGACTTCTCTTATTGTGAGAGCAATACTTGTTCTTACGGGCTAATGAACAACTTTCAGGTGATGAGCATGGTGTCAGGATTTGCACCCTTGATCTCTGCAGGGATCTTTTCAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCAGCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCGCTTCGTGG--TTACATCTTAACATTTCTGATTGCACTGGGATTCATCCTGATTGCTGAATTGAACGTTATTGCTCCAATTATCTCTAATTTCTTCCTTGCATCGTATGCCTTAATCAACTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCTGCATTCAAATACTACAACATGTGGATCTCCCTTATTGGAGCAATTCTTTGTTGCATAGTGATGTTTGTCATTAACTGGTGGGCTGCGTTGCTCACATATGTGATAGTCCTTGGGCTGTACATTTATGTTACTTACAAAAAACCAGATGTGAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGTGCACTGCAGCATTCAATCCGTCTTTCTGGGGTGGAGGACCATGTGAAAAACTTCAGACCACAGTGCCTTGTAATGACAGGTTCTCCAAACTCACGGCCTGCTTTACTTCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGTGCATATGGGCCCCAGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAGATATCAGCGATGGCTCATTAAAAACAAAATGAAGGCTTTCTATGCTCCGGTTCACGCAGATGACTTGCGAGAAGGTGCACAATACTTGATGCAGGCTGCTGGACTTGGTCGTATGAAGCCAAACACACTTGTCCTGGGATTTAAGAAAGATTGGTTACAAG-CCGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATTTGGAGTTGTGGTTATCCGCCTGAAGGAAGGACTGGATATATCACACCTCCAAGGTCAGGAAGAATTATTATCTTCACAAGAAAAATCACCTGGTACCAAGGATGTGGTAGTAAATGTGGATTACAGTAAAAAGTCAGATCAAGATACTTGCAAATCATCTGGTGAAAAATCCATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTCCAACTCAGCCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACCAAAAGCTTCTTGAAGCTAGCACACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGGAGGTTTGACATTGTTGATACCCTATCTTCTGACTACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTATTCATTGGTGGAAAAATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTATTGAGTAAATTCCGGATAGACTTCTCTGATATCATGGTCCTTGGAGATATCAACACCAAGCCAAAAAAAGAAAATATTATTGCTTTTGATGACATGATCGAGCCGTACAGACTTCACGAAGATGATAAAGAACAGGACATTGCAGATAAGATGAAAGAAGATGAGCCTTGGCGAATCACAGATAATGAGCTTGAGCTTTATAAGACTAAGACATATCGACAGATCAGGTTAAATGAATTATTAAAGGAGCATTCAAGCACAGCTAACATTATTGTCATGAGTCTTCCAGTCGCACGGAAAGGCGCTGTGTCCAGTGCTCTGTATATGGCTTGGTTAGAAGCTCTCTCGAAGGACCTGCCACCAGTCCTCCTGGTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCATAA

Amino Acid Sequence

MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU38401
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee