cDNA Clone MBS85; PP1 beta

• Plasmid: POPH-His-PP1 beta, GST-MBS85
• Parent Plasmid: pOPH 6His
• Expressed: His-PP1 beta, GST-MBS85
• Genbank: PP1 beta BT019744(human AA), NM_001101722 (rabbit) MYPT2: NM_002481.2
• Tag: GST

Sequence of Insert

DNA sequence of rabbit PP1 beta

ATGGCTCATCATCACCATCACCACATGGAAAACCTGTATTTTCAGGGCCATATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGTACGAGGATGTCGTCCAGGAAAGATTGTGCAAATGACTGAAGCGGAAGTGCGGGGCCTTTGTATCAAGTCTCGGGAGATCTTCCTCAGCCAGCCTATTCTCTTGGAATTGGAAGCACCACTGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAGTATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGATAGAGGAAAACAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTACAAAATCAAATATCCAGAGAACTTCTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAGCGAAGGTTTAATATTAAATTGTGGAAGACCTTCACTGACTGCTTTAACTGTCTGCCTATAGCTGCCATTGTGGATGAGAAGATCTTCTGTTGTCATGGAGGACTGTCACCAGACCTACAATCTATGGAGCAGATTCGGAGAATTATGAGACCCACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTACTGTGGTCTGACCCAGACAAGGACGTGCAAGGCTGGGGAGAAAATGACCGTGGTGTTTCCTTCACTTTTGGCGCTGATGTAGTCAGTAAATTTCTGAATCGTCATGATTTAGACCTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTTTGCTAAGCGACAATTGGTAACCCTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGATGAGACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAGAAAGCTAAATACCAGTATGGTGGACTGAATTCTGGACGCCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAGGTGAGGATCCAGATCTAAGCTTGGTACC

GST-MYPT3
GGATCCAATAATTTTGTTTAACTTTAAGAAGGAGATATAAATATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTCGGAGCCCCAGGAATTCCCGGGGCGACTCGAGCGGCCGCCAGATCTTCCGGATGGCTCGAGTTTTTCAGCAAGATGGCCGCGATGAGCGGCGAAGATGGTCCCGCCGCAGGGCCTGGTGCAGCTGCTGCCGCTGCCCGAGAACGCAGACGAGAACAGCTCAGACAGTGGGGTGCTCGCGCCGGCGCTGAACCTGGTCCAGGGGAACGCAGAGCTCGGACCGTGAGGTTTGAGCGAGCTGCCGAGTTCCTGGCCGCTTGTGCCGGCGGGGACCTGGATGAAGCCCGACTCATGCTGCGCGCTGCCGATCCAGGCCCGGGGGCAGAACTCGATCCTGCTGCCCCACCCCCTGCTCGAGCCGTGCTGGACTCTACAAATGCCGATGGCATTAGCGCACTGCACCAGGCCTGTATCGATGAAAACCTGGAAGTGGTCAGGTTTCTGGTGGAGCAGGGCGCCACCGTGAATCAGGCCGATAATGAGGGCTGGACACCTCTGCACGTGGCCGCTTCTTGTGGCTATCTGGATATCGCCAGGTACCTCCTGAGCCACGGGGCCAACATCGCCGCCGTCAACAGTGACGGGGACCTGCCCCTGGACCTGGCCGAGTCGGACGCCATGGAGGGGCTGCTGAAGGCGGAGATCGCCCGCCGAGGTGTGGATGTGGAAGCAGCCAAGCGGGCAGAAGAGGAATTGCTCCTTCATGACACGAGGTGCTGGCTGAATGGGGGCGCCATGCCAGAGGCCCGGCACCCCCGCACAGGCGCCTCTGCCCTGCACGTGGCTGCTGCCAAGGGCTACATTGAGGTGATGAGGTTGCTCCTTCAGGCTGGCTACGACCCAGAGCTCCGGGACGGGGACGGCTGGACTCCCCTGCACGCAGCGGCACACTGGGGCGTGGAGGATGCCTGCCGCCTGCTGGCCGAGCATGGCGGGGGCATGGACTCACTGACCCATGCGGGGCAGCGTCCCTGTGACCTGGCCGATGAGGAAGTACTGAGCCTGTTGGAGGAACTGGCCCGGAAACAGGAGGACCTTCGGAACCAAAAAGAAGCTTCCCAGAGCCGGGGCCAGGAGCCCCAAGCGCCCTCTAGCAGCAAACACAGAAGGAGCTCTGTGTGTCGTCTGAGCAGTCGCGAGAAGATTTCCCTCCAGGACTTGTCCAAGGAGCGCCGGCCTGGTGGGGCTGGGGGGCCCCCCATCCAGGACGAGGATGAGGGGGAAGAAGGTCCCACCGAACCACCCCCTGCAGAACCCAGAACCCTCAATGGCGTCTCCTCCCCGCCGCACCCCAGCCCTAAGAGTCCCGTGCAGCTTGAAGAGGCCCCCTTCTCCAGGCGCTTTGGCCTCCTGAAGACAGGGAGTTCTGGTGCCCTGGGTCCCCCTGAAAGGCGGACAGCGGAGGGAGCCCCTGGGGCTGGGCTGCAGCGCTCGGCTTCCTCCTCCTGGCTGGAAGGGACCTCCACTCAGGCCAAGGAGCTCCGTCTTGCCAGAATTACCCCGACCCCCTCCCCGAAGCTGCCGGAGCCCTCTGTCCTGTCTGAGGTCACCAAGCCTCCTCCTTGCTTGGAGAACTCCTCGCCTCCCTCCAGGATTCCGGAGCCTGAATCCCCAGCGAAGCCAAACGTCCCCACAGCCTCCACGGCGCCCCCAGCGGACTCCCGGGACCGACGGAGGTCCTACCAGATGCCTGTGCGGGATGAGGAGTCGGAATCCCAGAGAAAAGCTCGCTCCCGTCTCATGCGCCAGTCTCGGAGGTCCACACAGGGTGTGACTCTTACAGACCTGAAGGAGGCAGAGAAGGCTGCAGGGAAGGCCCCAGAGTCAGAGAAGCCGGCGCAGAGCCTGGACCCTTCCCGAAGGCCCCGCGTCCCTGGAGTGGAGAACTCTGACAGCCCTGCCCAGAGAGCAGAGGCGCCCGACGGGCAGGGTCCGGGACCGCAGGCGGCCAGGGAGCACCGCAAGGTCGGAAAGGAGTGGAGGGGGCCTGCGGAGGGGGAGGAGGCGGAGCCGGCTGACCGCAGCCAGGAGTCCAGCACCCTGGAGGGCGGCCCCTCGGCCCGCAGGCAGCGGTGGCAGCGGGACCTCAACCCAGAACCTGAGCCAGAATCGGAAGAGCCAGACGGAGGCTTTAGGACGCTGTATGCAGAGCTGCGCAGGGAGAACGAGCGGCTTCGCGAGGCCCTGACCGAGACCACGCTGCGGCTGGCGCAGCTCAAGGTGGAGCTGGAGCGGGCCACGCAGAGGCAAGAACGCTTCGCTGAGAGGCCAGCCCTCCTGGAACTGGAGAGATTCGAGCGCAGGGCCCTGGAACGCAAGGCCGCAGAGCTGGAGGAGGAGCTGAAGGCCCTGTCTGACCTCCGCGCTGACAACCAGCGCCTCAAGGATGAGAATGCAGCGTTGATCCGCGTCATCAGCAAACTCTCCAAGTGAACGGCT

Amino Acid Sequence

MAHHHHHHMENLYFQGHMADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

GST-MBS85
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGAPGIPGATRAAARSSGWLEFFSKMAAMSGEDGPAAGPGAAAAAARERRREQLRQWGARAGAEPGPGERRARTVRFERAAEFLAACAGGDLDEARLMLRAADPGPGAELDPAAPPPARAVLDSTNADGISALHQACIDENLEVVRFLVEQGATVNQADNEGWTPLHVAASCGYLDIARYLLSHGANIAAVNSDGDLPLDLAESDAMEGLLKAEIARRGVDVEAAKRAEEELLLHDTRCWLNGGAMPEARHPRTGASALHVAAAKGYIEVMRLLLQAGYDPELRDGDGWTPLHAAAHWGVEDACRLLAEHGGGMDSLTHAGQRPCDLADEEVLSLLEELARKQEDLRNQKEASQSRGQEPQAPSSSKHRRSSVCRLSSREKISLQDLSKERRPGGAGGPPIQDEDEGEEGPTEPPPAEPRTLNGVSSPPHPSPKSPVQLEEAPFSRRFGLLKTGSSGALGPPERRTAEGAPGAGLQRSASSSWLEGTSTQAKELRLARITPTPSPKLPEPSVLSEVTKPPPCLENSSPPSRIPEPESPAKPNVPTASTAPPADSRDRRRSYQMPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAEKAAGKAPESEKPAQSLDPSRRPRVPGVENSDSPAQRAEAPDGQGPGPQAAREHRKVGKEWRGPAEGEEAEPADRSQESSTLEGGPSARRQRWQRDLNPEPEPESEEPDGGFRTLYAELRRENERLREALTETTLRLAQLKVELERATQRQERFAERPALLELERFERRALERKAAELEEELKALSDLRADNQRLKDENAALIRVISKLSK
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU34101
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Species : PP1: rabbit DNA; human AA seq